Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nrfA and Shewana3_0659 are separated by 175 nucleotides Shewana3_0659 and Shewana3_0660 are separated by 205 nucleotides
Shewana3_0658: nrfA - cytochrome c552 (RefSeq), at 778,296 to 779,717
nrfA
Shewana3_0659: Shewana3_0659 - diguanylate cyclase/phosphodiesterase with GAF sensor (RefSeq), at 779,893 to 781,833
_0659
Shewana3_0660: Shewana3_0660 - hypothetical protein (RefSeq), at 782,039 to 782,809
_0660
Position (kb) 779
780
781
782 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 779.042 kb on + strand, within nrfA at 779.042 kb on + strand, within nrfA at 779.050 kb on - strand, within nrfA at 779.050 kb on - strand, within nrfA at 779.072 kb on - strand, within nrfA at 779.333 kb on + strand, within nrfA at 779.391 kb on - strand, within nrfA at 779.496 kb on - strand, within nrfA at 779.534 kb on + strand, within nrfA at 779.612 kb on + strand at 779.633 kb on - strand at 779.885 kb on - strand at 779.898 kb on + strand at 779.929 kb on - strand at 780.162 kb on - strand, within Shewana3_0659 at 780.166 kb on + strand, within Shewana3_0659 at 780.166 kb on + strand, within Shewana3_0659 at 780.174 kb on - strand, within Shewana3_0659 at 780.174 kb on - strand, within Shewana3_0659 at 780.330 kb on - strand, within Shewana3_0659 at 780.419 kb on - strand, within Shewana3_0659 at 780.443 kb on - strand, within Shewana3_0659 at 780.523 kb on - strand, within Shewana3_0659 at 780.558 kb on + strand, within Shewana3_0659 at 780.723 kb on - strand, within Shewana3_0659 at 780.743 kb on + strand, within Shewana3_0659 at 780.795 kb on + strand, within Shewana3_0659 at 780.795 kb on + strand, within Shewana3_0659 at 780.890 kb on + strand, within Shewana3_0659 at 780.913 kb on + strand, within Shewana3_0659 at 781.134 kb on - strand, within Shewana3_0659 at 781.203 kb on + strand, within Shewana3_0659 at 781.259 kb on - strand, within Shewana3_0659 at 781.398 kb on + strand, within Shewana3_0659 at 781.406 kb on - strand, within Shewana3_0659 at 781.524 kb on - strand, within Shewana3_0659 at 781.539 kb on + strand, within Shewana3_0659 at 781.578 kb on - strand, within Shewana3_0659 at 781.705 kb on + strand at 781.718 kb on - strand at 781.870 kb on + strand at 781.884 kb on - strand at 782.053 kb on - strand at 782.132 kb on + strand, within Shewana3_0660 at 782.187 kb on + strand, within Shewana3_0660 at 782.202 kb on - strand, within Shewana3_0660 at 782.284 kb on - strand, within Shewana3_0660 at 782.294 kb on + strand, within Shewana3_0660 at 782.294 kb on + strand, within Shewana3_0660 at 782.294 kb on + strand, within Shewana3_0660 at 782.368 kb on - strand, within Shewana3_0660 at 782.368 kb on - strand, within Shewana3_0660 at 782.368 kb on - strand, within Shewana3_0660 at 782.369 kb on + strand, within Shewana3_0660 at 782.397 kb on + strand, within Shewana3_0660 at 782.582 kb on + strand, within Shewana3_0660 at 782.623 kb on + strand, within Shewana3_0660 at 782.633 kb on + strand, within Shewana3_0660 at 782.752 kb on + strand at 782.784 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 779,042 + nrfA Shewana3_0658 0.52 -0.5 779,042 + nrfA Shewana3_0658 0.52 +0.1 779,050 - nrfA Shewana3_0658 0.53 -0.6 779,050 - nrfA Shewana3_0658 0.53 +0.6 779,072 - nrfA Shewana3_0658 0.55 -0.2 779,333 + nrfA Shewana3_0658 0.73 -1.9 779,391 - nrfA Shewana3_0658 0.77 -0.0 779,496 - nrfA Shewana3_0658 0.84 +0.9 779,534 + nrfA Shewana3_0658 0.87 +0.8 779,612 + -0.8 779,633 - +0.6 779,885 - -0.0 779,898 + +1.2 779,929 - +0.6 780,162 - Shewana3_0659 0.14 -0.9 780,166 + Shewana3_0659 0.14 +0.1 780,166 + Shewana3_0659 0.14 -1.6 780,174 - Shewana3_0659 0.14 -2.0 780,174 - Shewana3_0659 0.14 +0.3 780,330 - Shewana3_0659 0.23 +0.3 780,419 - Shewana3_0659 0.27 +0.1 780,443 - Shewana3_0659 0.28 -1.1 780,523 - Shewana3_0659 0.32 +0.1 780,558 + Shewana3_0659 0.34 +0.5 780,723 - Shewana3_0659 0.43 -1.1 780,743 + Shewana3_0659 0.44 +0.5 780,795 + Shewana3_0659 0.46 -0.9 780,795 + Shewana3_0659 0.46 +0.5 780,890 + Shewana3_0659 0.51 +0.0 780,913 + Shewana3_0659 0.53 +1.1 781,134 - Shewana3_0659 0.64 +0.2 781,203 + Shewana3_0659 0.67 +0.1 781,259 - Shewana3_0659 0.70 +0.2 781,398 + Shewana3_0659 0.78 +0.5 781,406 - Shewana3_0659 0.78 -2.4 781,524 - Shewana3_0659 0.84 +0.6 781,539 + Shewana3_0659 0.85 -0.6 781,578 - Shewana3_0659 0.87 -0.7 781,705 + +0.4 781,718 - +0.9 781,870 + +0.5 781,884 - -0.8 782,053 - -0.7 782,132 + Shewana3_0660 0.12 +0.2 782,187 + Shewana3_0660 0.19 +0.3 782,202 - Shewana3_0660 0.21 -2.1 782,284 - Shewana3_0660 0.32 -0.5 782,294 + Shewana3_0660 0.33 -0.6 782,294 + Shewana3_0660 0.33 +0.7 782,294 + Shewana3_0660 0.33 -1.3 782,368 - Shewana3_0660 0.43 +0.1 782,368 - Shewana3_0660 0.43 -4.1 782,368 - Shewana3_0660 0.43 +0.6 782,369 + Shewana3_0660 0.43 +0.5 782,397 + Shewana3_0660 0.46 -0.5 782,582 + Shewana3_0660 0.70 +0.4 782,623 + Shewana3_0660 0.76 +0.6 782,633 + Shewana3_0660 0.77 -1.5 782,752 + +0.4 782,784 + +0.7
Or see this region's nucleotide sequence