Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt miaA and Shewana3_0597 are separated by 92 nucleotides Shewana3_0597 and Shewana3_0598 are separated by 23 nucleotides Shewana3_0598 and Shewana3_0599 are separated by 36 nucleotides
Shewana3_0596: miaA - tRNA delta(2)-isopentenylpyrophosphate transferase (RefSeq), at 699,889 to 700,779
miaA
Shewana3_0597: Shewana3_0597 - RNA-binding protein Hfq (RefSeq), at 700,872 to 701,144
_0597
Shewana3_0598: Shewana3_0598 - GTP-binding protein, HSR1-related (RefSeq), at 701,168 to 702,475
_0598
Shewana3_0599: Shewana3_0599 - HflK protein (RefSeq), at 702,512 to 703,657
_0599
Position (kb)
701
702
703 Strain fitness (log2 ratio)
-2
-1
0
1 at 701.053 kb on + strand, within Shewana3_0597 at 701.275 kb on + strand at 701.290 kb on + strand at 701.806 kb on + strand, within Shewana3_0598 at 702.538 kb on + strand at 702.657 kb on + strand, within Shewana3_0599 at 702.657 kb on + strand, within Shewana3_0599 at 702.777 kb on + strand, within Shewana3_0599 at 702.955 kb on + strand, within Shewana3_0599 at 702.992 kb on + strand, within Shewana3_0599 at 703.432 kb on + strand, within Shewana3_0599
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 701,053 + Shewana3_0597 0.66 -1.5 701,275 + -2.3 701,290 + -0.9 701,806 + Shewana3_0598 0.49 +0.6 702,538 + +0.4 702,657 + Shewana3_0599 0.13 +1.2 702,657 + Shewana3_0599 0.13 -1.1 702,777 + Shewana3_0599 0.23 -0.8 702,955 + Shewana3_0599 0.39 +0.4 702,992 + Shewana3_0599 0.42 +1.0 703,432 + Shewana3_0599 0.80 +0.9
Or see this region's nucleotide sequence