Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0590 and Shewana3_R0024 are separated by 117 nucleotides Shewana3_R0024 and Shewana3_R0025 are separated by 33 nucleotides Shewana3_R0025 and Shewana3_R0026 are separated by 123 nucleotides Shewana3_R0026 and Shewana3_R0027 are separated by 44 nucleotides Shewana3_R0027 and Shewana3_0591 are separated by 562 nucleotides
Shewana3_0590: Shewana3_0590 - oligoribonuclease (RefSeq), at 692,034 to 692,579
_0590
Shewana3_R0024: Shewana3_R0024 - tRNA-Gly (RefSeq), at 692,697 to 692,772
_R0024
Shewana3_R0025: Shewana3_R0025 - tRNA-Gly (RefSeq), at 692,806 to 692,881
_R0025
Shewana3_R0026: Shewana3_R0026 - tRNA-Gly (RefSeq), at 693,005 to 693,080
_R0026
Shewana3_R0027: Shewana3_R0027 - tRNA-Gly (RefSeq), at 693,125 to 693,200
_R0027
Shewana3_0591: Shewana3_0591 - hypothetical protein (RefSeq), at 693,763 to 694,218
_0591
Position (kb) 693
694 Strain fitness (log2 ratio) -2
-1
0
1
2 at 692.475 kb on - strand, within Shewana3_0590 at 692.685 kb on + strand at 693.163 kb on + strand, within Shewana3_R0027 at 693.299 kb on + strand at 693.391 kb on + strand at 693.421 kb on - strand at 693.428 kb on + strand at 693.522 kb on + strand at 693.734 kb on + strand at 693.741 kb on + strand at 693.741 kb on + strand at 693.741 kb on + strand at 693.746 kb on + strand at 693.749 kb on - strand at 693.823 kb on + strand, within Shewana3_0591 at 694.021 kb on - strand, within Shewana3_0591
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 692,475 - Shewana3_0590 0.81 -0.6 692,685 + +2.7 693,163 + Shewana3_R0027 0.50 +0.4 693,299 + -0.5 693,391 + +0.4 693,421 - +0.0 693,428 + +0.0 693,522 + +0.2 693,734 + -2.1 693,741 + +0.5 693,741 + +0.1 693,741 + +0.4 693,746 + -1.0 693,749 - -1.1 693,823 + Shewana3_0591 0.13 +0.9 694,021 - Shewana3_0591 0.57 -0.3
Or see this region's nucleotide sequence