Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0375 and Shewana3_R0020 are separated by 208 nucleotides Shewana3_R0020 and Shewana3_R0021 are separated by 53 nucleotides Shewana3_R0021 and Shewana3_R0022 are separated by 14 nucleotides Shewana3_R0022 and Shewana3_R0023 are separated by 53 nucleotides Shewana3_R0023 and Shewana3_0376 are separated by 189 nucleotides
Shewana3_0375: Shewana3_0375 - diguanylate cyclase/phosphodiesterase with GAF sensor (RefSeq), at 413,613 to 416,243
_0375
Shewana3_R0020: Shewana3_R0020 - tRNA-Arg (RefSeq), at 416,452 to 416,528
_R0020
Shewana3_R0021: Shewana3_R0021 - tRNA-His (RefSeq), at 416,582 to 416,657
_R0021
Shewana3_R0022: Shewana3_R0022 - tRNA-Pro (RefSeq), at 416,672 to 416,748
_R0022
Shewana3_R0023: Shewana3_R0023 - tRNA-Pro (RefSeq), at 416,802 to 416,878
_R0023
Shewana3_0376: Shewana3_0376 - diguanylate cyclase/phosphodiesterase (RefSeq), at 417,068 to 418,987
_0376
Position (kb) 416
417 Strain fitness (log2 ratio) -1
0
1 at 415.927 kb on + strand, within Shewana3_0375 at 416.057 kb on + strand at 416.148 kb on - strand at 417.114 kb on + strand at 417.461 kb on + strand, within Shewana3_0376 at 417.463 kb on + strand, within Shewana3_0376 at 417.505 kb on - strand, within Shewana3_0376 at 417.597 kb on + strand, within Shewana3_0376 at 417.618 kb on + strand, within Shewana3_0376 at 417.618 kb on + strand, within Shewana3_0376 at 417.618 kb on + strand, within Shewana3_0376 at 417.830 kb on - strand, within Shewana3_0376
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 415,927 + Shewana3_0375 0.88 -0.1 416,057 + +0.2 416,148 - +0.1 417,114 + -1.1 417,461 + Shewana3_0376 0.20 -0.0 417,463 + Shewana3_0376 0.21 +0.5 417,505 - Shewana3_0376 0.23 +0.1 417,597 + Shewana3_0376 0.28 +0.4 417,618 + Shewana3_0376 0.29 -1.4 417,618 + Shewana3_0376 0.29 -1.2 417,618 + Shewana3_0376 0.29 +0.3 417,830 - Shewana3_0376 0.40 -1.1
Or see this region's nucleotide sequence