Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0312 and Shewana3_0313 overlap by 4 nucleotides Shewana3_0313 and Shewana3_0314 are separated by 15 nucleotides Shewana3_0314 and Shewana3_0315 are separated by 39 nucleotides Shewana3_0315 and Shewana3_0316 are separated by 133 nucleotides
Shewana3_0312: Shewana3_0312 - prolyl aminopeptidase (RefSeq), at 350,384 to 351,289
_0312
Shewana3_0313: Shewana3_0313 - hypothetical protein (RefSeq), at 351,286 to 351,888
_0313
Shewana3_0314: Shewana3_0314 - D-tyrosyl-tRNA(Tyr) deacylase (RefSeq), at 351,904 to 352,341
_0314
Shewana3_0315: Shewana3_0315 - thioesterase domain-containing protein (RefSeq), at 352,381 to 352,818
_0315
Shewana3_0316: Shewana3_0316 - azoreductase (RefSeq), at 352,952 to 353,548
_0316
Position (kb) 351
352
353 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 350.933 kb on + strand, within Shewana3_0312 at 350.983 kb on + strand, within Shewana3_0312 at 351.436 kb on + strand, within Shewana3_0313 at 351.482 kb on - strand, within Shewana3_0313 at 351.529 kb on - strand, within Shewana3_0313 at 351.575 kb on - strand, within Shewana3_0313 at 351.787 kb on - strand, within Shewana3_0313 at 351.844 kb on - strand at 352.055 kb on + strand, within Shewana3_0314 at 352.191 kb on + strand, within Shewana3_0314 at 352.380 kb on - strand at 352.413 kb on - strand at 352.447 kb on - strand, within Shewana3_0315 at 352.526 kb on - strand, within Shewana3_0315 at 352.621 kb on + strand, within Shewana3_0315 at 352.629 kb on - strand, within Shewana3_0315 at 352.654 kb on + strand, within Shewana3_0315 at 352.654 kb on + strand, within Shewana3_0315 at 352.662 kb on - strand, within Shewana3_0315 at 352.662 kb on - strand, within Shewana3_0315 at 352.765 kb on + strand, within Shewana3_0315 at 352.800 kb on - strand at 352.909 kb on - strand at 353.023 kb on + strand, within Shewana3_0316 at 353.115 kb on + strand, within Shewana3_0316 at 353.173 kb on + strand, within Shewana3_0316 at 353.202 kb on - strand, within Shewana3_0316 at 353.291 kb on + strand, within Shewana3_0316
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 350,933 + Shewana3_0312 0.61 -0.8 350,983 + Shewana3_0312 0.66 -0.8 351,436 + Shewana3_0313 0.25 +0.6 351,482 - Shewana3_0313 0.33 +0.5 351,529 - Shewana3_0313 0.40 +0.1 351,575 - Shewana3_0313 0.48 +0.0 351,787 - Shewana3_0313 0.83 -2.7 351,844 - +1.1 352,055 + Shewana3_0314 0.34 +1.0 352,191 + Shewana3_0314 0.66 -0.6 352,380 - +0.6 352,413 - +0.4 352,447 - Shewana3_0315 0.15 -1.6 352,526 - Shewana3_0315 0.33 -0.9 352,621 + Shewana3_0315 0.55 -0.1 352,629 - Shewana3_0315 0.57 -1.1 352,654 + Shewana3_0315 0.62 -0.4 352,654 + Shewana3_0315 0.62 -0.1 352,662 - Shewana3_0315 0.64 +0.2 352,662 - Shewana3_0315 0.64 -2.9 352,765 + Shewana3_0315 0.88 -0.4 352,800 - -0.3 352,909 - -0.8 353,023 + Shewana3_0316 0.12 -1.1 353,115 + Shewana3_0316 0.27 -1.3 353,173 + Shewana3_0316 0.37 +0.5 353,202 - Shewana3_0316 0.42 +1.3 353,291 + Shewana3_0316 0.57 +0.1
Or see this region's nucleotide sequence