Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0179 and Shewana3_0180 are separated by 141 nucleotides Shewana3_0180 and Shewana3_0181 are separated by 11 nucleotides
Shewana3_0179: Shewana3_0179 - tRNA (uracil-5-)-methyltransferase (RefSeq), at 212,709 to 213,806
_0179
Shewana3_0180: Shewana3_0180 - glutamate racemase (RefSeq), at 213,948 to 214,769
_0180
Shewana3_0181: Shewana3_0181 - RNP-1-like RNA-binding protein (RefSeq), at 214,781 to 215,245
_0181
Position (kb) 213
214
215 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 212.966 kb on + strand, within Shewana3_0179 at 213.100 kb on - strand, within Shewana3_0179 at 213.436 kb on - strand, within Shewana3_0179 at 213.563 kb on - strand, within Shewana3_0179 at 213.699 kb on + strand at 213.701 kb on + strand at 213.715 kb on + strand at 213.732 kb on + strand at 213.745 kb on - strand at 213.889 kb on + strand at 213.889 kb on + strand at 214.833 kb on + strand, within Shewana3_0181 at 214.841 kb on - strand, within Shewana3_0181 at 214.929 kb on - strand, within Shewana3_0181 at 214.930 kb on - strand, within Shewana3_0181 at 214.988 kb on - strand, within Shewana3_0181 at 214.988 kb on - strand, within Shewana3_0181 at 215.016 kb on - strand, within Shewana3_0181 at 215.084 kb on + strand, within Shewana3_0181 at 215.404 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 212,966 + Shewana3_0179 0.23 -4.5 213,100 - Shewana3_0179 0.36 +0.4 213,436 - Shewana3_0179 0.66 -0.5 213,563 - Shewana3_0179 0.78 -0.3 213,699 + +1.0 213,701 + +0.4 213,715 + -1.3 213,732 + +0.3 213,745 - +0.8 213,889 + +0.2 213,889 + -0.2 214,833 + Shewana3_0181 0.11 -2.5 214,841 - Shewana3_0181 0.13 -1.0 214,929 - Shewana3_0181 0.32 +1.1 214,930 - Shewana3_0181 0.32 +0.1 214,988 - Shewana3_0181 0.45 +0.1 214,988 - Shewana3_0181 0.45 -0.0 215,016 - Shewana3_0181 0.51 +0.2 215,084 + Shewana3_0181 0.65 -1.0 215,404 - -2.6
Or see this region's nucleotide sequence