Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0052 and Shewana3_0053 are separated by 260 nucleotides Shewana3_0053 and gpsA are separated by 4 nucleotides gpsA and Shewana3_0055 are separated by 238 nucleotides
Shewana3_0052: Shewana3_0052 - rhodanese domain-containing protein (RefSeq), at 61,994 to 62,470
_0052
Shewana3_0053: Shewana3_0053 - preprotein translocase subunit SecB (RefSeq), at 62,731 to 63,216
_0053
Shewana3_0054: gpsA - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase (RefSeq), at 63,221 to 64,237
gpsA
Shewana3_0055: Shewana3_0055 - hypothetical protein (RefSeq), at 64,476 to 65,660
_0055
Position (kb) 63
64
65 Strain fitness (log2 ratio) -2
-1
0
1 at 62.255 kb on + strand, within Shewana3_0052 at 62.332 kb on + strand, within Shewana3_0052 at 62.332 kb on + strand, within Shewana3_0052 at 62.484 kb on + strand at 62.494 kb on + strand at 64.344 kb on - strand at 64.397 kb on + strand at 64.404 kb on - strand at 64.474 kb on - strand at 64.518 kb on + strand at 64.744 kb on + strand, within Shewana3_0055 at 64.765 kb on + strand, within Shewana3_0055 at 64.767 kb on - strand, within Shewana3_0055 at 64.846 kb on + strand, within Shewana3_0055 at 64.925 kb on + strand, within Shewana3_0055 at 64.988 kb on + strand, within Shewana3_0055 at 64.989 kb on + strand, within Shewana3_0055 at 64.989 kb on + strand, within Shewana3_0055 at 65.178 kb on + strand, within Shewana3_0055 at 65.186 kb on - strand, within Shewana3_0055
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 62,255 + Shewana3_0052 0.55 -0.1 62,332 + Shewana3_0052 0.71 -2.0 62,332 + Shewana3_0052 0.71 -2.6 62,484 + -0.7 62,494 + -0.3 64,344 - -1.3 64,397 + -0.8 64,404 - -1.4 64,474 - +0.8 64,518 + +0.5 64,744 + Shewana3_0055 0.23 +0.5 64,765 + Shewana3_0055 0.24 +0.1 64,767 - Shewana3_0055 0.25 -0.7 64,846 + Shewana3_0055 0.31 +0.5 64,925 + Shewana3_0055 0.38 +0.5 64,988 + Shewana3_0055 0.43 +0.5 64,989 + Shewana3_0055 0.43 -0.4 64,989 + Shewana3_0055 0.43 -0.8 65,178 + Shewana3_0055 0.59 +0.2 65,186 - Shewana3_0055 0.60 +0.4
Or see this region's nucleotide sequence