Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3372 and Shew_3373 are separated by 225 nucleotides Shew_3373 and Shew_3374 are separated by 220 nucleotides Shew_3374 and Shew_3375 overlap by 4 nucleotides
Shew_3372: Shew_3372 - arginase/agmatinase/formiminoglutamase (RefSeq), at 4,021,340 to 4,022,383
_3372
Shew_3373: Shew_3373 - ubiquinone/menaquinone biosynthesis methyltransferase (RefSeq), at 4,022,609 to 4,023,364
_3373
Shew_3374: Shew_3374 - hypothetical protein (RefSeq), at 4,023,585 to 4,024,208
_3374
Shew_3375: Shew_3375 - 2-polyprenylphenol 6-hydroxylase (RefSeq), at 4,024,205 to 4,025,854
_3375
Position (kb) 4022
4023
4024 Strain fitness (log2 ratio) -2
-1
0
1 at 4021.637 kb on - strand, within Shew_3372 at 4021.659 kb on + strand, within Shew_3372 at 4021.659 kb on + strand, within Shew_3372 at 4021.690 kb on + strand, within Shew_3372 at 4021.698 kb on - strand, within Shew_3372 at 4022.323 kb on - strand at 4022.419 kb on + strand at 4023.691 kb on + strand, within Shew_3374 at 4023.821 kb on + strand, within Shew_3374 at 4024.099 kb on + strand, within Shew_3374
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,021,637 - Shew_3372 0.28 +1.1 4,021,659 + Shew_3372 0.31 +0.2 4,021,659 + Shew_3372 0.31 -0.5 4,021,690 + Shew_3372 0.34 +0.3 4,021,698 - Shew_3372 0.34 +0.1 4,022,323 - +0.3 4,022,419 + -1.3 4,023,691 + Shew_3374 0.17 -1.8 4,023,821 + Shew_3374 0.38 -1.6 4,024,099 + Shew_3374 0.82 -2.2
Or see this region's nucleotide sequence