Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2919 and Shew_2920 are separated by 70 nucleotides Shew_2920 and Shew_2921 are separated by 103 nucleotides Shew_2921 and Shew_2922 are separated by 198 nucleotides Shew_2922 and Shew_2923 are separated by 98 nucleotides
Shew_2919: Shew_2919 - translation-associated GTPase (RefSeq), at 3,457,797 to 3,458,888
_2919
Shew_2920: Shew_2920 - peptidyl-tRNA hydrolase (RefSeq), at 3,458,959 to 3,459,546
_2920
Shew_2921: Shew_2921 - UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase (RefSeq), at 3,459,650 to 3,460,843
_2921
Shew_2922: Shew_2922 - hypothetical protein (RefSeq), at 3,461,042 to 3,461,344
_2922
Shew_2923: Shew_2923 - tRNA-i(6)A37 thiotransferase enzyme MiaB (RefSeq), at 3,461,443 to 3,462,867
_2923
Position (kb) 3459
3460
3461 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 3458.828 kb on - strand at 3460.829 kb on - strand at 3461.144 kb on - strand, within Shew_2922 at 3461.205 kb on + strand, within Shew_2922 at 3461.236 kb on + strand, within Shew_2922 at 3461.498 kb on + strand at 3461.660 kb on + strand, within Shew_2923
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,458,828 - +0.4 3,460,829 - -2.5 3,461,144 - Shew_2922 0.34 +0.5 3,461,205 + Shew_2922 0.54 -2.8 3,461,236 + Shew_2922 0.64 -0.1 3,461,498 + +0.4 3,461,660 + Shew_2923 0.15 -0.6
Or see this region's nucleotide sequence