Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2534 and Shew_2535 are separated by 226 nucleotides Shew_2535 and Shew_2536 are separated by 40 nucleotides Shew_2536 and Shew_2537 are separated by 307 nucleotides
Shew_2534: Shew_2534 - glycerate kinase (RefSeq), at 2,991,493 to 2,992,638
_2534
Shew_2535: Shew_2535 - pyridoxal-dependent decarboxylase (RefSeq), at 2,992,865 to 2,994,505
_2535
Shew_2536: Shew_2536 - putative thiol-disulphide oxidoreductase DCC (RefSeq), at 2,994,546 to 2,995,016
_2536
Shew_2537: Shew_2537 - AraC family transcriptional regulator (RefSeq), at 2,995,324 to 2,996,262
_2537
Position (kb)
2992
2993
2994
2995 Strain fitness (log2 ratio)
-1
0
1
2 at 2991.875 kb on - strand, within Shew_2534 at 2992.336 kb on - strand, within Shew_2534 at 2992.336 kb on - strand, within Shew_2534 at 2992.402 kb on - strand, within Shew_2534 at 2992.402 kb on - strand, within Shew_2534 at 2992.649 kb on + strand at 2992.785 kb on - strand at 2993.619 kb on + strand, within Shew_2535 at 2994.378 kb on + strand at 2994.655 kb on + strand, within Shew_2536 at 2994.737 kb on + strand, within Shew_2536 at 2994.958 kb on + strand, within Shew_2536 at 2995.031 kb on + strand at 2995.127 kb on - strand at 2995.157 kb on + strand at 2995.435 kb on - strand, within Shew_2537
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,991,875 - Shew_2534 0.33 -0.6 2,992,336 - Shew_2534 0.74 -1.0 2,992,336 - Shew_2534 0.74 -0.7 2,992,402 - Shew_2534 0.79 -0.1 2,992,402 - Shew_2534 0.79 -0.1 2,992,649 + +0.1 2,992,785 - +0.8 2,993,619 + Shew_2535 0.46 +0.8 2,994,378 + +2.3 2,994,655 + Shew_2536 0.23 +0.7 2,994,737 + Shew_2536 0.41 -0.3 2,994,958 + Shew_2536 0.87 -0.2 2,995,031 + +0.4 2,995,127 - +0.4 2,995,157 + -0.5 2,995,435 - Shew_2537 0.12 +1.8
Or see this region's nucleotide sequence