Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2022 and Shew_2023 are separated by 58 nucleotides Shew_2023 and Shew_2024 are separated by 157 nucleotides Shew_2024 and Shew_2025 are separated by 182 nucleotides Shew_2025 and Shew_2026 are separated by 179 nucleotides
Shew_2022: Shew_2022 - recombination factor protein RarA (RefSeq), at 2,334,482 to 2,335,813
_2022
Shew_2023: Shew_2023 - outer membrane lipoprotein carrier protein LolA (RefSeq), at 2,335,872 to 2,336,498
_2023
Shew_2024: Shew_2024 - cell divisionFtsK/SpoIIIE (RefSeq), at 2,336,656 to 2,339,181
_2024
Shew_2025: Shew_2025 - leucine-responsive transcriptional regulator (RefSeq), at 2,339,364 to 2,339,867
_2025
Shew_2026: Shew_2026 - alanine dehydrogenase (RefSeq), at 2,340,047 to 2,341,162
_2026
Position (kb)
2336
2337
2338
2339
2340 Strain fitness (log2 ratio)
-1
0
1 at 2336.604 kb on - strand at 2337.458 kb on - strand, within Shew_2024 at 2339.270 kb on + strand at 2339.395 kb on + strand at 2339.465 kb on + strand, within Shew_2025 at 2339.746 kb on - strand, within Shew_2025 at 2339.750 kb on + strand, within Shew_2025 at 2339.753 kb on - strand, within Shew_2025 at 2339.758 kb on - strand, within Shew_2025 at 2339.758 kb on - strand, within Shew_2025
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,336,604 - -0.5 2,337,458 - Shew_2024 0.32 -1.3 2,339,270 + -0.0 2,339,395 + +0.4 2,339,465 + Shew_2025 0.20 -0.7 2,339,746 - Shew_2025 0.76 +0.1 2,339,750 + Shew_2025 0.77 -0.8 2,339,753 - Shew_2025 0.77 +0.5 2,339,758 - Shew_2025 0.78 -1.5 2,339,758 - Shew_2025 0.78 +1.1
Or see this region's nucleotide sequence