Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1922 and Shew_1923 are separated by 158 nucleotides Shew_1923 and Shew_1924 are separated by 319 nucleotides Shew_1924 and Shew_1925 are separated by 183 nucleotides
Shew_1922: Shew_1922 - phosphoglucomutase (RefSeq), at 2,218,643 to 2,220,322
_1922
Shew_1923: Shew_1923 - peptide methionine sulfoxide reductase (RefSeq), at 2,220,481 to 2,220,960
_1923
Shew_1924: Shew_1924 - succinylglutamate desuccinylase (RefSeq), at 2,221,280 to 2,222,347
_1924
Shew_1925: Shew_1925 - 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) (RefSeq), at 2,222,531 to 2,223,709
_1925
Position (kb)
2221
2222
2223 Strain fitness (log2 ratio)
-2
-1
0
1 at 2220.388 kb on - strand at 2220.824 kb on + strand, within Shew_1923 at 2220.842 kb on - strand, within Shew_1923 at 2221.086 kb on + strand at 2221.235 kb on + strand at 2222.301 kb on + strand at 2222.404 kb on + strand at 2222.869 kb on - strand, within Shew_1925 at 2222.883 kb on + strand, within Shew_1925 at 2223.060 kb on - strand, within Shew_1925 at 2223.096 kb on + strand, within Shew_1925 at 2223.119 kb on + strand, within Shew_1925 at 2223.127 kb on - strand, within Shew_1925 at 2223.127 kb on - strand, within Shew_1925 at 2223.334 kb on + strand, within Shew_1925
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,220,388 - +1.0 2,220,824 + Shew_1923 0.71 -0.3 2,220,842 - Shew_1923 0.75 -0.5 2,221,086 + -0.2 2,221,235 + +0.1 2,222,301 + +0.5 2,222,404 + +0.3 2,222,869 - Shew_1925 0.29 +0.2 2,222,883 + Shew_1925 0.30 -0.3 2,223,060 - Shew_1925 0.45 -0.7 2,223,096 + Shew_1925 0.48 +0.3 2,223,119 + Shew_1925 0.50 +0.2 2,223,127 - Shew_1925 0.51 -0.1 2,223,127 - Shew_1925 0.51 -2.0 2,223,334 + Shew_1925 0.68 +1.6
Or see this region's nucleotide sequence