Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1899 and asnC are separated by 651 nucleotides asnC and Shew_1901 are separated by 246 nucleotides Shew_1901 and Shew_1902 are separated by 111 nucleotides
Shew_1899: Shew_1899 - hypothetical protein (RefSeq), at 2,193,844 to 2,194,353
_1899
Shew_1900: asnC - asparaginyl-tRNA synthetase (RefSeq), at 2,195,005 to 2,196,405
asnC
Shew_1901: Shew_1901 - hypothetical protein (RefSeq), at 2,196,652 to 2,196,987
_1901
Shew_1902: Shew_1902 - NUDIX hydrolase (RefSeq), at 2,197,099 to 2,197,668
_1902
Position (kb)
2195
2196
2197 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2194.219 kb on + strand, within Shew_1899 at 2194.240 kb on - strand, within Shew_1899 at 2194.247 kb on - strand, within Shew_1899 at 2194.368 kb on + strand at 2194.371 kb on + strand at 2194.455 kb on + strand at 2194.455 kb on + strand at 2194.463 kb on - strand at 2194.463 kb on - strand at 2194.465 kb on + strand at 2194.473 kb on - strand at 2194.473 kb on - strand at 2194.473 kb on - strand at 2194.473 kb on - strand at 2194.626 kb on - strand at 2194.757 kb on + strand at 2194.865 kb on + strand at 2194.902 kb on + strand at 2196.566 kb on + strand at 2196.594 kb on + strand at 2196.752 kb on - strand, within Shew_1901 at 2196.841 kb on + strand, within Shew_1901 at 2197.052 kb on - strand at 2197.052 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,194,219 + Shew_1899 0.74 -1.1 2,194,240 - Shew_1899 0.78 -0.2 2,194,247 - Shew_1899 0.79 +0.6 2,194,368 + +0.2 2,194,371 + -0.7 2,194,455 + -0.7 2,194,455 + +0.3 2,194,463 - -0.4 2,194,463 - +2.3 2,194,465 + +0.3 2,194,473 - -0.6 2,194,473 - +0.0 2,194,473 - +0.7 2,194,473 - -2.1 2,194,626 - -0.1 2,194,757 + +0.2 2,194,865 + -0.5 2,194,902 + +0.3 2,196,566 + +0.3 2,196,594 + -0.1 2,196,752 - Shew_1901 0.30 +0.3 2,196,841 + Shew_1901 0.56 +1.1 2,197,052 - +0.6 2,197,052 - -2.2
Or see this region's nucleotide sequence