Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1587 and Shew_1588 are separated by 29 nucleotides Shew_1588 and rbgA are separated by 170 nucleotides rbgA and Shew_1590 are separated by 108 nucleotides
Shew_1587: Shew_1587 - DNA ligase (RefSeq), at 1,817,076 to 1,817,888
_1587
Shew_1588: Shew_1588 - protein tyrosine phosphatase (RefSeq), at 1,817,918 to 1,818,382
_1588
Shew_1589: rbgA - ribosomal biogenesis GTPase (RefSeq), at 1,818,553 to 1,819,494
rbgA
Shew_1590: Shew_1590 - 2OG-Fe(II) oxygenase (RefSeq), at 1,819,603 to 1,820,442
_1590
Position (kb)
1818
1819
1820 Strain fitness (log2 ratio)
-1
0
1 at 1817.580 kb on - strand, within Shew_1587 at 1817.600 kb on + strand, within Shew_1587 at 1817.612 kb on + strand, within Shew_1587 at 1817.926 kb on + strand at 1817.934 kb on - strand at 1818.176 kb on - strand, within Shew_1588 at 1818.253 kb on - strand, within Shew_1588 at 1818.334 kb on - strand, within Shew_1588 at 1818.334 kb on - strand, within Shew_1588 at 1818.559 kb on - strand at 1819.652 kb on + strand at 1820.310 kb on + strand, within Shew_1590 at 1820.402 kb on + strand at 1820.407 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,817,580 - Shew_1587 0.62 -0.5 1,817,600 + Shew_1587 0.64 -0.1 1,817,612 + Shew_1587 0.66 +0.2 1,817,926 + -0.2 1,817,934 - +0.2 1,818,176 - Shew_1588 0.55 +0.5 1,818,253 - Shew_1588 0.72 -0.6 1,818,334 - Shew_1588 0.89 +0.5 1,818,334 - Shew_1588 0.89 -1.1 1,818,559 - -0.2 1,819,652 + -0.7 1,820,310 + Shew_1590 0.84 +0.3 1,820,402 + +0.3 1,820,407 + +0.1
Or see this region's nucleotide sequence