Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1543 and Shew_1544 are separated by 47 nucleotides Shew_1544 and Shew_1545 are separated by 153 nucleotides Shew_1545 and Shew_1546 are separated by 250 nucleotides Shew_1546 and Shew_1547 are separated by 153 nucleotides
Shew_1543: Shew_1543 - secretion protein HlyD family protein (RefSeq), at 1,771,815 to 1,772,873
_1543
Shew_1544: Shew_1544 - TetR family transcriptional regulator (RefSeq), at 1,772,921 to 1,773,580
_1544
Shew_1545: Shew_1545 - two component LuxR family transcriptional regulator (RefSeq), at 1,773,734 to 1,774,393
_1545
Shew_1546: Shew_1546 - thioesterase superfamily protein (RefSeq), at 1,774,644 to 1,775,069
_1546
Shew_1547: Shew_1547 - phospho-2-dehydro-3-deoxyheptonate aldolase (RefSeq), at 1,775,223 to 1,776,284
_1547
Position (kb)
1773
1774
1775 Strain fitness (log2 ratio)
-1
0
1 at 1772.735 kb on - strand, within Shew_1543 at 1773.049 kb on + strand, within Shew_1544 at 1773.066 kb on + strand, within Shew_1544 at 1773.096 kb on + strand, within Shew_1544 at 1773.686 kb on + strand at 1773.740 kb on - strand at 1774.197 kb on - strand, within Shew_1545 at 1774.574 kb on + strand at 1774.574 kb on + strand at 1774.937 kb on - strand, within Shew_1546
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,772,735 - Shew_1543 0.87 +0.3 1,773,049 + Shew_1544 0.19 -0.8 1,773,066 + Shew_1544 0.22 -1.1 1,773,096 + Shew_1544 0.27 +0.7 1,773,686 + -0.0 1,773,740 - +0.8 1,774,197 - Shew_1545 0.70 +0.3 1,774,574 + +0.1 1,774,574 + -0.2 1,774,937 - Shew_1546 0.69 +0.0
Or see this region's nucleotide sequence