Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1427 and Shew_1428 are separated by 28 nucleotides Shew_1428 and Shew_1429 are separated by 74 nucleotides Shew_1429 and Shew_1430 are separated by 12 nucleotides Shew_1430 and Shew_1431 are separated by 172 nucleotides
Shew_1427: Shew_1427 - 3'(2'),5'-bisphosphate nucleotidase (RefSeq), at 1,643,151 to 1,643,945
_1427
Shew_1428: Shew_1428 - fructokinase (RefSeq), at 1,643,974 to 1,644,864
_1428
Shew_1429: Shew_1429 - hypothetical protein (RefSeq), at 1,644,939 to 1,645,679
_1429
Shew_1430: Shew_1430 - hypothetical protein (RefSeq), at 1,645,692 to 1,646,360
_1430
Shew_1431: Shew_1431 - hypothetical protein (RefSeq), at 1,646,533 to 1,646,988
_1431
Position (kb)
1644
1645
1646 Strain fitness (log2 ratio)
-1
0
1
2 at 1644.477 kb on + strand, within Shew_1428 at 1644.920 kb on - strand at 1645.179 kb on + strand, within Shew_1429 at 1645.602 kb on - strand, within Shew_1429 at 1645.744 kb on + strand at 1645.744 kb on + strand at 1645.752 kb on - strand at 1645.897 kb on - strand, within Shew_1430 at 1646.249 kb on + strand, within Shew_1430 at 1646.249 kb on + strand, within Shew_1430 at 1646.249 kb on + strand, within Shew_1430 at 1646.249 kb on + strand, within Shew_1430 at 1646.257 kb on - strand, within Shew_1430 at 1646.257 kb on - strand, within Shew_1430 at 1646.257 kb on - strand, within Shew_1430 at 1646.257 kb on - strand, within Shew_1430 at 1646.355 kb on - strand at 1646.367 kb on + strand at 1646.375 kb on - strand at 1646.395 kb on + strand at 1646.540 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,644,477 + Shew_1428 0.56 -0.4 1,644,920 - -0.4 1,645,179 + Shew_1429 0.32 +0.6 1,645,602 - Shew_1429 0.89 +0.8 1,645,744 + -1.0 1,645,744 + -0.2 1,645,752 - +0.2 1,645,897 - Shew_1430 0.31 +0.3 1,646,249 + Shew_1430 0.83 +0.4 1,646,249 + Shew_1430 0.83 +0.8 1,646,249 + Shew_1430 0.83 -0.1 1,646,249 + Shew_1430 0.83 -0.3 1,646,257 - Shew_1430 0.84 -0.0 1,646,257 - Shew_1430 0.84 +0.8 1,646,257 - Shew_1430 0.84 -0.0 1,646,257 - Shew_1430 0.84 +0.1 1,646,355 - -0.4 1,646,367 + +0.3 1,646,375 - +2.4 1,646,395 + -0.3 1,646,540 + +0.7
Or see this region's nucleotide sequence