Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nhaR and Shew_1045 are separated by 64 nucleotides Shew_1045 and Shew_1046 overlap by 20 nucleotides Shew_1046 and Shew_1047 are separated by 8 nucleotides Shew_1047 and Shew_1048 are separated by 199 nucleotides Shew_1048 and Shew_1049 are separated by 39 nucleotides
Shew_1044: nhaR - transcriptional activator NhaR (RefSeq), at 1,207,778 to 1,208,686
nhaR
Shew_1045: Shew_1045 - hypothetical protein (RefSeq), at 1,208,751 to 1,209,002
_1045
Shew_1046: Shew_1046 - hypothetical protein (RefSeq), at 1,208,983 to 1,209,438
_1046
Shew_1047: Shew_1047 - L-aspartate oxidase (RefSeq), at 1,209,447 to 1,211,060
_1047
Shew_1048: Shew_1048 - RNA polymerase sigma factor RpoE (RefSeq), at 1,211,260 to 1,211,838
_1048
Shew_1049: Shew_1049 - anti sigma-E protein, RseA (RefSeq), at 1,211,878 to 1,212,480
_1049
Position (kb) 1209
1210
1211
1212 Strain fitness (log2 ratio) -2
-1
0
1 at 1208.830 kb on + strand, within Shew_1045 at 1209.010 kb on - strand at 1209.020 kb on + strand at 1209.121 kb on - strand, within Shew_1046 at 1209.177 kb on + strand, within Shew_1046 at 1209.191 kb on + strand, within Shew_1046 at 1211.746 kb on + strand, within Shew_1048 at 1211.791 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,208,830 + Shew_1045 0.31 -2.2 1,209,010 - -1.2 1,209,020 + +0.3 1,209,121 - Shew_1046 0.30 +0.5 1,209,177 + Shew_1046 0.43 -0.2 1,209,191 + Shew_1046 0.46 +0.2 1,211,746 + Shew_1048 0.84 -0.8 1,211,791 - -2.6
Or see this region's nucleotide sequence