Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1043 and nhaR are separated by 20 nucleotides nhaR and Shew_1045 are separated by 64 nucleotides Shew_1045 and Shew_1046 overlap by 20 nucleotides Shew_1046 and Shew_1047 are separated by 8 nucleotides
Shew_1043: Shew_1043 - hypothetical protein (RefSeq), at 1,207,374 to 1,207,757
_1043
Shew_1044: nhaR - transcriptional activator NhaR (RefSeq), at 1,207,778 to 1,208,686
nhaR
Shew_1045: Shew_1045 - hypothetical protein (RefSeq), at 1,208,751 to 1,209,002
_1045
Shew_1046: Shew_1046 - hypothetical protein (RefSeq), at 1,208,983 to 1,209,438
_1046
Shew_1047: Shew_1047 - L-aspartate oxidase (RefSeq), at 1,209,447 to 1,211,060
_1047
Position (kb) 1208
1209
1210 Strain fitness (log2 ratio) -2
-1
0
1 at 1207.760 kb on + strand at 1207.865 kb on - strand at 1207.930 kb on - strand, within nhaR at 1208.162 kb on - strand, within nhaR at 1208.331 kb on + strand, within nhaR at 1208.830 kb on + strand, within Shew_1045 at 1209.010 kb on - strand at 1209.020 kb on + strand at 1209.121 kb on - strand, within Shew_1046 at 1209.177 kb on + strand, within Shew_1046 at 1209.191 kb on + strand, within Shew_1046
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,207,760 + +0.8 1,207,865 - +0.8 1,207,930 - nhaR Shew_1044 0.17 +0.5 1,208,162 - nhaR Shew_1044 0.42 +0.5 1,208,331 + nhaR Shew_1044 0.61 +1.4 1,208,830 + Shew_1045 0.31 -2.2 1,209,010 - -1.2 1,209,020 + +0.3 1,209,121 - Shew_1046 0.30 +0.5 1,209,177 + Shew_1046 0.43 -0.2 1,209,191 + Shew_1046 0.46 +0.2
Or see this region's nucleotide sequence