Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1021 and Shew_1022 are separated by 126 nucleotides Shew_1022 and Shew_1023 are separated by 146 nucleotides Shew_1023 and Shew_1024 are separated by 1 nucleotides
Shew_1021: Shew_1021 - peptidase M23B (RefSeq), at 1,179,811 to 1,181,286
_1021
Shew_1022: Shew_1022 - tyrosyl-tRNA synthetase (RefSeq), at 1,181,413 to 1,182,609
_1022
Shew_1023: Shew_1023 - hypothetical protein (RefSeq), at 1,182,756 to 1,183,235
_1023
Shew_1024: Shew_1024 - hypothetical protein (RefSeq), at 1,183,237 to 1,183,857
_1024
Position (kb)
1181
1182
1183 Strain fitness (log2 ratio)
-1
0
1 at 1180.436 kb on + strand, within Shew_1021 at 1180.775 kb on + strand, within Shew_1021 at 1180.781 kb on + strand, within Shew_1021 at 1180.832 kb on + strand, within Shew_1021 at 1180.850 kb on - strand, within Shew_1021 at 1180.933 kb on + strand, within Shew_1021 at 1180.979 kb on - strand, within Shew_1021 at 1182.871 kb on - strand, within Shew_1023 at 1183.044 kb on + strand, within Shew_1023 at 1183.065 kb on - strand, within Shew_1023 at 1183.233 kb on + strand at 1183.373 kb on - strand, within Shew_1024 at 1183.373 kb on - strand, within Shew_1024 at 1183.588 kb on + strand, within Shew_1024
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,180,436 + Shew_1021 0.42 +0.4 1,180,775 + Shew_1021 0.65 -0.2 1,180,781 + Shew_1021 0.66 +0.6 1,180,832 + Shew_1021 0.69 +0.3 1,180,850 - Shew_1021 0.70 +0.4 1,180,933 + Shew_1021 0.76 +0.4 1,180,979 - Shew_1021 0.79 +0.3 1,182,871 - Shew_1023 0.24 -0.1 1,183,044 + Shew_1023 0.60 -0.6 1,183,065 - Shew_1023 0.64 +0.3 1,183,233 + +0.5 1,183,373 - Shew_1024 0.22 +0.2 1,183,373 - Shew_1024 0.22 +0.1 1,183,588 + Shew_1024 0.57 +0.0
Or see this region's nucleotide sequence