Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0561 and Shew_0562 are separated by 100 nucleotides Shew_0562 and Shew_0563 overlap by 14 nucleotides Shew_0563 and Shew_0564 are separated by 9 nucleotides
Shew_0561: Shew_0561 - carbohydrate kinase, YjeF-related protein (RefSeq), at 635,897 to 637,375
_0561
Shew_0562: Shew_0562 - hypothetical protein (RefSeq), at 637,476 to 637,949
_0562
Shew_0563: Shew_0563 - N-acetylmuramoyl-L-alanine amidase (RefSeq), at 637,936 to 639,255
_0563
Shew_0564: Shew_0564 - DNA mismatch repair protein MutL (RefSeq), at 639,265 to 641,160
_0564
Position (kb) 637
638
639
640 Strain fitness (log2 ratio) -1
0
1 at 637.105 kb on + strand, within Shew_0561 at 637.369 kb on + strand at 637.369 kb on + strand at 637.377 kb on - strand at 637.377 kb on - strand at 638.948 kb on + strand, within Shew_0563 at 639.209 kb on - strand at 639.355 kb on + strand at 639.363 kb on - strand at 639.363 kb on - strand at 639.478 kb on - strand, within Shew_0564 at 639.494 kb on + strand, within Shew_0564 at 639.494 kb on + strand, within Shew_0564 at 639.494 kb on + strand, within Shew_0564 at 639.613 kb on - strand, within Shew_0564 at 639.638 kb on - strand, within Shew_0564 at 639.652 kb on - strand, within Shew_0564 at 639.962 kb on + strand, within Shew_0564 at 640.178 kb on + strand, within Shew_0564 at 640.186 kb on - strand, within Shew_0564 at 640.225 kb on - strand, within Shew_0564
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 637,105 + Shew_0561 0.82 -0.3 637,369 + -0.1 637,369 + +0.3 637,377 - -0.6 637,377 - -0.4 638,948 + Shew_0563 0.77 +0.2 639,209 - -0.0 639,355 + -0.4 639,363 - +0.3 639,363 - -0.8 639,478 - Shew_0564 0.11 +0.1 639,494 + Shew_0564 0.12 -0.2 639,494 + Shew_0564 0.12 +0.2 639,494 + Shew_0564 0.12 +0.3 639,613 - Shew_0564 0.18 -0.2 639,638 - Shew_0564 0.20 -0.2 639,652 - Shew_0564 0.20 +0.6 639,962 + Shew_0564 0.37 +0.1 640,178 + Shew_0564 0.48 +0.5 640,186 - Shew_0564 0.49 -0.3 640,225 - Shew_0564 0.51 -1.0
Or see this region's nucleotide sequence