Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0225 and Shew_0226 are separated by 68 nucleotides Shew_0226 and Shew_0227 overlap by 4 nucleotides Shew_0227 and Shew_0228 are separated by 88 nucleotides
Shew_0224: Shew_0224 - transport-associated (RefSeq), at 237,981 to 238,550
_0224
Shew_0225: Shew_0225 - phosphoheptose isomerase (RefSeq), at 238,547 to 239,140
_0225
Shew_0226: Shew_0226 - hypothetical protein (RefSeq), at 239,209 to 239,553
_0226
Shew_0227: Shew_0227 - LppC family lipoprotein (RefSeq), at 239,550 to 241,454
_0227
Shew_0228: Shew_0228 - uroporphyrin-III C/tetrapyrrole methyltransferase (RefSeq), at 241,543 to 242,385
_0228
Position (kb) 239
240
241
242 Strain fitness (log2 ratio) -2
-1
0
1 at 239.042 kb on + strand, within Shew_0225 at 239.254 kb on - strand, within Shew_0226 at 239.495 kb on - strand, within Shew_0226 at 240.078 kb on + strand, within Shew_0227 at 240.482 kb on - strand, within Shew_0227 at 241.621 kb on - strand at 241.771 kb on + strand, within Shew_0228 at 241.921 kb on - strand, within Shew_0228 at 242.158 kb on - strand, within Shew_0228 at 242.233 kb on + strand, within Shew_0228 at 242.331 kb on - strand at 242.416 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 239,042 + Shew_0225 0.83 -2.0 239,254 - Shew_0226 0.13 +0.3 239,495 - Shew_0226 0.83 -0.8 240,078 + Shew_0227 0.28 +0.5 240,482 - Shew_0227 0.49 -0.1 241,621 - -1.4 241,771 + Shew_0228 0.27 -0.5 241,921 - Shew_0228 0.45 -0.0 242,158 - Shew_0228 0.73 -1.6 242,233 + Shew_0228 0.82 -1.5 242,331 - -0.1 242,416 + +0.0
Or see this region's nucleotide sequence