Strain Fitness in Paraburkholderia graminis OAS925 around ABIE53_003539

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
3,978,331	+				+2.4
3,978,332	-				-0.6
3,978,424	+		ABIE53_003538	0.20	-0.7
3,978,424	+		ABIE53_003538	0.20	+1.0
3,978,424	+		ABIE53_003538	0.20	+0.8
3,978,424	+		ABIE53_003538	0.20	-2.1
3,978,424	+		ABIE53_003538	0.20	+0.0
3,978,424	+		ABIE53_003538	0.20	-0.3
3,978,424	+		ABIE53_003538	0.20	-1.1
3,978,424	+		ABIE53_003538	0.20	-0.1
3,978,424	+		ABIE53_003538	0.20	-1.2
3,978,424	+		ABIE53_003538	0.20	-0.2
3,978,425	-		ABIE53_003538	0.21	-1.5
3,978,425	-		ABIE53_003538	0.21	-0.5
3,978,425	-		ABIE53_003538	0.21	+2.7
3,978,425	-		ABIE53_003538	0.21	-0.9
3,978,425	-		ABIE53_003538	0.21	-2.0
3,978,425	-		ABIE53_003538	0.21	-0.9
3,978,595	+		ABIE53_003538	0.40	-0.2
3,978,595	+		ABIE53_003538	0.40	+4.1
3,978,595	+		ABIE53_003538	0.40	+0.3
3,978,596	-		ABIE53_003538	0.40	-1.5
3,978,647	+		ABIE53_003538	0.46	-1.9
3,978,648	-		ABIE53_003538	0.46	+1.7
3,978,667	+		ABIE53_003538	0.48	+1.1
3,978,667	+		ABIE53_003538	0.48	-0.0
3,978,667	+		ABIE53_003538	0.48	+0.4
3,978,667	+		ABIE53_003538	0.48	-1.1
3,978,667	+		ABIE53_003538	0.48	+1.0
3,978,667	+		ABIE53_003538	0.48	-0.1
3,978,667	+		ABIE53_003538	0.48	-0.2
3,978,667	+		ABIE53_003538	0.48	-1.5
3,978,667	+		ABIE53_003538	0.48	+0.3
3,978,667	+		ABIE53_003538	0.48	+0.9
3,978,667	+		ABIE53_003538	0.48	+0.4
3,978,668	-		ABIE53_003538	0.48	+0.2
3,978,668	-		ABIE53_003538	0.48	-0.9
3,978,668	-		ABIE53_003538	0.48	-0.4
3,978,668	-		ABIE53_003538	0.48	-1.1
3,978,668	-		ABIE53_003538	0.48	+0.1
3,978,668	-		ABIE53_003538	0.48	+3.1
3,978,668	-		ABIE53_003538	0.48	+0.7
3,978,668	-		ABIE53_003538	0.48	-1.2
3,978,668	-		ABIE53_003538	0.48	+0.6
3,978,668	-		ABIE53_003538	0.48	+2.1
3,978,668	-		ABIE53_003538	0.48	-0.2
3,978,757	+		ABIE53_003538	0.58	-0.4
3,978,757	+		ABIE53_003538	0.58	+1.1
3,978,758	-		ABIE53_003538	0.58	+0.1
3,978,758	-		ABIE53_003538	0.58	-1.2
3,978,758	-		ABIE53_003538	0.58	-0.6
3,978,758	-		ABIE53_003538	0.58	+0.1
3,978,758	-		ABIE53_003538	0.58	-0.7
3,978,758	-		ABIE53_003538	0.58	+0.2
3,978,772	+		ABIE53_003538	0.60	-0.5
3,978,772	+		ABIE53_003538	0.60	-1.5
3,978,772	+		ABIE53_003538	0.60	-0.4
3,978,773	-		ABIE53_003538	0.60	-0.5
3,978,773	-		ABIE53_003538	0.60	-0.5
3,978,773	-		ABIE53_003538	0.60	+3.7
3,978,773	-		ABIE53_003538	0.60	+0.1
3,978,835	+		ABIE53_003538	0.67	+0.1
3,978,836	-		ABIE53_003538	0.67	+0.1
3,978,836	-		ABIE53_003538	0.67	+0.5
3,978,896	+		ABIE53_003538	0.74	+0.1
3,978,896	+		ABIE53_003538	0.74	-1.1
3,978,896	+		ABIE53_003538	0.74	+3.1
3,978,896	+		ABIE53_003538	0.74	+1.1
3,978,896	+		ABIE53_003538	0.74	+0.1
3,978,896	+		ABIE53_003538	0.74	-0.3
3,978,897	-		ABIE53_003538	0.74	-2.5
3,978,897	-		ABIE53_003538	0.74	+0.5
3,978,897	-		ABIE53_003538	0.74	+2.5
3,978,897	-		ABIE53_003538	0.74	-0.7
3,978,924	-		ABIE53_003538	0.77	-0.7
3,978,925	+		ABIE53_003538	0.77	+0.9
3,978,925	+		ABIE53_003538	0.77	-0.5
3,978,925	+		ABIE53_003538	0.77	+1.5
3,978,925	+		ABIE53_003538	0.77	-1.3
3,978,926	-		ABIE53_003538	0.77	+3.7
3,978,926	-		ABIE53_003538	0.77	-1.3
3,978,926	-		ABIE53_003538	0.77	+0.5
3,978,926	-		ABIE53_003538	0.77	+0.5
3,978,926	-		ABIE53_003538	0.77	+0.0
3,978,926	-		ABIE53_003538	0.77	-1.5
3,978,936	-		ABIE53_003538	0.78	-0.4
3,978,980	+		ABIE53_003538	0.83	+0.7
3,979,039	+				+0.2
3,979,039	+				-0.2
3,979,039	+				-2.9
3,979,039	+				-0.2
3,979,039	+				+2.1
3,979,040	-				-0.9
3,979,040	-				-0.2
3,979,040	-				+1.7
3,979,040	-				+0.2
3,979,093	-				-0.2
3,979,093	-				+2.5
3,979,097	+				+0.1
3,979,097	+				-1.6
3,979,097	+				-0.7
3,979,098	-				+0.2
3,979,110	-				+0.1
3,979,162	+				-0.2
3,979,162	+				+0.5
3,979,163	-				+1.0
3,979,163	-				-3.2
3,979,163	-				-0.0
3,979,163	-				+2.1
3,979,169	+				+3.1
3,979,169	+				+0.3
3,979,169	+				+0.7
3,979,170	-				+0.0
3,979,170	-				-1.9
3,979,170	-				-1.2
3,979,170	-				-0.7
3,979,170	-				-0.7
3,979,170	-				-1.2
3,979,170	-				-0.9
3,979,170	-				-2.0
3,979,170	-				-0.2
3,979,170	-				+0.8
3,979,180	+				-0.5
3,979,224	+				+1.5
3,979,224	+				-0.1
3,979,224	+				+0.3
3,979,224	+				+2.1
3,979,224	+				+1.5
3,979,224	+				-0.2
3,979,224	+				-0.0
3,979,224	+				+0.7
3,979,224	+				-0.4
3,979,224	+				-0.8
3,979,224	+				-0.3
3,979,224	+				-0.9
3,979,224	+				-1.5
3,979,224	+				-0.1
3,979,224	+				-0.8
3,979,225	-				+0.5
3,979,225	-				+1.4
3,979,225	-				-1.8
3,979,225	-				+0.0
3,979,225	-				-0.2
3,979,225	-				-0.4
3,979,225	-				+0.6
3,979,225	-				+0.6
3,979,225	-				-0.4
3,979,225	-				-0.6
3,979,283	+		ABIE53_003539	0.10	-0.6
3,979,283	+		ABIE53_003539	0.10	+1.1
3,979,283	+		ABIE53_003539	0.10	-1.6
3,979,284	-		ABIE53_003539	0.10	+2.6
3,979,322	+		ABIE53_003539	0.20	+0.8
3,979,322	+		ABIE53_003539	0.20	-0.3
3,979,323	-		ABIE53_003539	0.20	-0.1
3,979,323	-		ABIE53_003539	0.20	+1.6
3,979,323	-		ABIE53_003539	0.20	-1.3
3,979,397	+		ABIE53_003539	0.38	-0.6
3,979,397	+		ABIE53_003539	0.38	-1.2
3,979,397	+		ABIE53_003539	0.38	-0.8
3,979,397	+		ABIE53_003539	0.38	+0.3
3,979,397	+		ABIE53_003539	0.38	+0.8
3,979,397	+		ABIE53_003539	0.38	-1.1
3,979,397	+		ABIE53_003539	0.38	-1.1
3,979,397	+		ABIE53_003539	0.38	-0.2
3,979,397	+		ABIE53_003539	0.38	-0.2
3,979,397	+		ABIE53_003539	0.38	-0.0
3,979,397	+		ABIE53_003539	0.38	-0.9
3,979,397	+		ABIE53_003539	0.38	+1.3
3,979,397	+		ABIE53_003539	0.38	-1.7
3,979,397	+		ABIE53_003539	0.38	-2.9
3,979,397	+		ABIE53_003539	0.38	-1.7
3,979,398	-		ABIE53_003539	0.38	-0.8
3,979,398	-		ABIE53_003539	0.38	+1.5
3,979,398	-		ABIE53_003539	0.38	-0.9
3,979,398	-		ABIE53_003539	0.38	-0.8
3,979,398	-		ABIE53_003539	0.38	-0.3
3,979,398	-		ABIE53_003539	0.38	+0.5
3,979,398	-		ABIE53_003539	0.38	+0.8
3,979,398	-		ABIE53_003539	0.38	-1.9
3,979,398	-		ABIE53_003539	0.38	+0.5
3,979,398	-		ABIE53_003539	0.38	+0.3
3,979,398	-		ABIE53_003539	0.38	-0.3
3,979,398	-		ABIE53_003539	0.38	+0.4
3,979,398	-		ABIE53_003539	0.38	+0.4
3,979,398	-		ABIE53_003539	0.38	-1.1
3,979,398	-		ABIE53_003539	0.38	+2.4
3,979,398	-		ABIE53_003539	0.38	-0.3
3,979,398	-		ABIE53_003539	0.38	-1.6
3,979,398	-		ABIE53_003539	0.38	+3.7
3,979,398	-		ABIE53_003539	0.38	+1.1
3,979,398	-		ABIE53_003539	0.38	+0.4
3,979,496	+		ABIE53_003539	0.62	+0.3
3,979,496	+		ABIE53_003539	0.62	-0.6
3,979,496	+		ABIE53_003539	0.62	+1.1
3,979,496	+		ABIE53_003539	0.62	-0.8
3,979,496	+		ABIE53_003539	0.62	+1.0
3,979,496	+		ABIE53_003539	0.62	-1.3
3,979,496	+		ABIE53_003539	0.62	-2.3
3,979,496	+		ABIE53_003539	0.62	-0.4
3,979,496	+		ABIE53_003539	0.62	+1.1
3,979,497	-		ABIE53_003539	0.62	-0.3
3,979,497	-		ABIE53_003539	0.62	-0.8
3,979,497	-		ABIE53_003539	0.62	+1.5
3,979,497	-		ABIE53_003539	0.62	+0.3
3,979,497	-		ABIE53_003539	0.62	+0.5
3,979,497	-		ABIE53_003539	0.62	+0.3
3,979,497	-		ABIE53_003539	0.62	-1.2
3,979,497	-		ABIE53_003539	0.62	-0.7
3,979,497	-		ABIE53_003539	0.62	+1.7
3,979,497	-		ABIE53_003539	0.62	+0.9
3,979,497	-		ABIE53_003539	0.62	-1.4
3,979,497	-		ABIE53_003539	0.62	+0.2
3,979,497	-		ABIE53_003539	0.62	+2.1
3,979,497	-		ABIE53_003539	0.62	+0.5
3,979,497	-		ABIE53_003539	0.62	-0.1
3,979,497	-		ABIE53_003539	0.62	-0.2
3,979,497	-		ABIE53_003539	0.62	+1.1
3,979,497	-		ABIE53_003539	0.62	+0.5
3,979,497	-		ABIE53_003539	0.62	-1.8
3,979,497	-		ABIE53_003539	0.62	+0.5
3,979,497	-		ABIE53_003539	0.62	-0.2
3,979,497	-		ABIE53_003539	0.62	-1.4
3,979,497	-		ABIE53_003539	0.62	-0.4
3,979,502	+		ABIE53_003539	0.63	+1.1
3,979,502	+		ABIE53_003539	0.63	-0.8
3,979,502	+		ABIE53_003539	0.63	-1.3
3,979,502	+		ABIE53_003539	0.63	-0.2
3,979,502	+		ABIE53_003539	0.63	+0.4
3,979,502	+		ABIE53_003539	0.63	-0.2
3,979,502	+		ABIE53_003539	0.63	+1.2
3,979,502	+		ABIE53_003539	0.63	-0.3
3,979,502	+		ABIE53_003539	0.63	-0.0
3,979,502	+		ABIE53_003539	0.63	+0.2
3,979,502	+		ABIE53_003539	0.63	-0.5
3,979,502	+		ABIE53_003539	0.63	-0.3
3,979,502	+		ABIE53_003539	0.63	-0.3
3,979,502	+		ABIE53_003539	0.63	+0.7
3,979,502	+		ABIE53_003539	0.63	-1.5
3,979,502	+		ABIE53_003539	0.63	+2.1
3,979,502	+		ABIE53_003539	0.63	+0.2
3,979,502	+		ABIE53_003539	0.63	+0.1
3,979,502	+		ABIE53_003539	0.63	+1.8
3,979,502	+		ABIE53_003539	0.63	+0.0
3,979,502	+		ABIE53_003539	0.63	+0.2
3,979,502	+		ABIE53_003539	0.63	-0.3
3,979,502	+		ABIE53_003539	0.63	-1.9
3,979,502	+		ABIE53_003539	0.63	-1.3
3,979,503	-		ABIE53_003539	0.63	-1.8
3,979,503	-		ABIE53_003539	0.63	+0.3
3,979,503	-		ABIE53_003539	0.63	-0.6
3,979,503	-		ABIE53_003539	0.63	-0.2
3,979,503	-		ABIE53_003539	0.63	-2.1
3,979,622	+				-0.4
3,979,622	+				-0.7
3,979,657	+				-0.3
3,979,657	+				-1.7
3,979,657	+				-1.1
3,979,657	+				-0.5
3,979,657	+				+1.1
3,979,657	+				+2.9
3,979,657	+				-1.6
3,979,657	+				+0.2
3,979,657	+				-2.8
3,979,657	+				+2.1
3,979,657	+				+0.0
3,979,657	+				-0.3
3,979,658	-				-0.3
3,979,658	-				-0.5
3,979,658	-				-0.6
3,979,658	-				-0.4
3,979,658	-				-1.4
3,979,658	-				-0.5
3,979,658	-				-0.4
3,979,658	-				-2.8
3,979,664	+				+1.1
3,979,664	+				+0.0
3,979,665	-				-1.3
3,979,665	-				-1.1
3,979,665	-				-1.3
3,979,694	+				+0.2
3,979,694	+				+1.3
3,979,694	+				-0.6
3,979,694	+				+0.5
3,979,694	+				-0.3
3,979,694	+				-0.5
3,979,694	+				-1.5
3,979,695	-				+0.1
3,979,695	-				+0.5
3,979,695	-				-0.2
3,979,695	-				-0.2
3,979,812	+		ABIE53_003540	0.19	+1.7
3,979,812	+		ABIE53_003540	0.19	-0.2
3,979,812	+		ABIE53_003540	0.19	-0.5
3,979,812	+		ABIE53_003540	0.19	+0.8
3,979,812	+		ABIE53_003540	0.19	+1.1
3,979,812	+		ABIE53_003540	0.19	+1.2
3,979,812	+		ABIE53_003540	0.19	-0.4
3,979,813	-		ABIE53_003540	0.19	-1.2
3,979,813	-		ABIE53_003540	0.19	-1.1
3,979,813	-		ABIE53_003540	0.19	-0.4
3,979,813	-		ABIE53_003540	0.19	-0.8
3,979,813	-		ABIE53_003540	0.19	+0.7
3,979,813	-		ABIE53_003540	0.19	-2.4
3,979,813	-		ABIE53_003540	0.19	+1.0
3,979,813	-		ABIE53_003540	0.19	+0.1
3,979,813	-		ABIE53_003540	0.19	-0.5
3,979,813	-		ABIE53_003540	0.19	-1.0
3,979,855	+		ABIE53_003540	0.24	+2.3
3,979,855	+		ABIE53_003540	0.24	-0.9
3,979,896	+		ABIE53_003540	0.30	-1.5
3,980,453	+				-0.1
3,980,453	+				-0.4
3,980,453	+				-1.5
3,980,454	-				+4.7
3,980,454	-				+0.9
3,980,454	-				-0.7
3,980,454	-				-0.1
3,980,454	-				-1.2
3,980,591	+				+1.5
3,980,591	+				+0.1
3,980,592	-				+0.1
3,980,592	-				+0.9
3,980,592	-				+0.1
3,980,596	+				+0.1
3,980,596	+				+1.2
3,980,596	+				+1.5
3,980,597	-				+0.6
3,980,597	-				-0.1
3,980,597	-				-0.5
3,980,597	-				-0.3
3,980,597	-				-0.6
3,980,613	-				-0.2
3,980,634	+				+2.1
3,980,635	-				-0.5
3,980,635	-				+2.1
3,980,635	-				-1.1
3,980,635	-				+1.1
3,980,635	-				-1.9