Experiment: LB_DAP agar plate, outgrowth in LB_gent
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_002867 and ABIE53_002868 are separated by 122 nucleotides ABIE53_002868 and ABIE53_002869 overlap by 1 nucleotides ABIE53_002869 and ABIE53_002870 are separated by 22 nucleotides
ABIE53_002867: ABIE53_002867 - 2-isopropylmalate synthase, at 3,239,610 to 3,241,328
_002867
ABIE53_002868: ABIE53_002868 - cystine transport system ATP-binding protein, at 3,241,451 to 3,242,230
_002868
ABIE53_002869: ABIE53_002869 - cystine transport system permease protein, at 3,242,230 to 3,242,907
_002869
ABIE53_002870: ABIE53_002870 - cystine transport system substrate-binding protein, at 3,242,930 to 3,243,712
_002870
Position (kb)
3241
3242
3243 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 3240.480 kb on + strand, within ABIE53_002867 at 3240.480 kb on + strand, within ABIE53_002867 at 3240.480 kb on + strand, within ABIE53_002867 at 3240.480 kb on + strand, within ABIE53_002867 at 3240.581 kb on + strand, within ABIE53_002867 at 3240.582 kb on + strand, within ABIE53_002867 at 3240.582 kb on + strand, within ABIE53_002867 at 3240.582 kb on + strand, within ABIE53_002867 at 3240.582 kb on + strand, within ABIE53_002867 at 3240.583 kb on - strand, within ABIE53_002867 at 3240.583 kb on - strand, within ABIE53_002867 at 3240.583 kb on - strand, within ABIE53_002867 at 3240.583 kb on - strand, within ABIE53_002867 at 3240.684 kb on + strand, within ABIE53_002867 at 3240.684 kb on + strand, within ABIE53_002867 at 3240.684 kb on + strand, within ABIE53_002867 at 3240.684 kb on + strand, within ABIE53_002867 at 3240.684 kb on + strand, within ABIE53_002867 at 3240.684 kb on + strand, within ABIE53_002867 at 3240.685 kb on - strand, within ABIE53_002867 at 3240.685 kb on - strand, within ABIE53_002867 at 3240.685 kb on - strand, within ABIE53_002867 at 3240.720 kb on + strand, within ABIE53_002867 at 3240.720 kb on + strand, within ABIE53_002867 at 3240.720 kb on + strand, within ABIE53_002867 at 3240.720 kb on + strand, within ABIE53_002867 at 3240.720 kb on + strand, within ABIE53_002867 at 3240.720 kb on + strand, within ABIE53_002867 at 3240.720 kb on + strand, within ABIE53_002867 at 3240.720 kb on + strand, within ABIE53_002867 at 3240.720 kb on + strand, within ABIE53_002867 at 3240.720 kb on + strand, within ABIE53_002867 at 3240.720 kb on + strand, within ABIE53_002867 at 3240.721 kb on - strand, within ABIE53_002867 at 3240.721 kb on - strand, within ABIE53_002867 at 3240.721 kb on - strand, within ABIE53_002867 at 3240.721 kb on - strand, within ABIE53_002867 at 3240.771 kb on + strand, within ABIE53_002867 at 3240.771 kb on + strand, within ABIE53_002867 at 3240.772 kb on - strand, within ABIE53_002867 at 3240.790 kb on - strand, within ABIE53_002867 at 3240.909 kb on + strand, within ABIE53_002867 at 3240.909 kb on + strand, within ABIE53_002867 at 3240.909 kb on + strand, within ABIE53_002867 at 3240.910 kb on - strand, within ABIE53_002867 at 3240.910 kb on - strand, within ABIE53_002867 at 3240.910 kb on - strand, within ABIE53_002867 at 3240.910 kb on - strand, within ABIE53_002867 at 3240.910 kb on - strand, within ABIE53_002867 at 3240.910 kb on - strand, within ABIE53_002867 at 3240.936 kb on + strand, within ABIE53_002867 at 3240.936 kb on + strand, within ABIE53_002867 at 3240.936 kb on + strand, within ABIE53_002867 at 3240.936 kb on + strand, within ABIE53_002867 at 3240.936 kb on + strand, within ABIE53_002867 at 3240.936 kb on + strand, within ABIE53_002867 at 3240.936 kb on + strand, within ABIE53_002867 at 3240.948 kb on - strand, within ABIE53_002867 at 3240.977 kb on + strand, within ABIE53_002867 at 3240.977 kb on + strand, within ABIE53_002867 at 3240.977 kb on + strand, within ABIE53_002867 at 3240.978 kb on - strand, within ABIE53_002867 at 3241.089 kb on + strand, within ABIE53_002867 at 3241.225 kb on + strand at 3241.248 kb on + strand at 3241.248 kb on + strand at 3241.248 kb on + strand at 3241.249 kb on - strand at 3241.249 kb on - strand at 3241.326 kb on + strand at 3241.326 kb on + strand at 3241.326 kb on + strand at 3241.326 kb on + strand at 3241.327 kb on - strand at 3241.327 kb on - strand at 3241.327 kb on - strand at 3241.327 kb on - strand at 3241.337 kb on + strand at 3241.337 kb on + strand at 3241.337 kb on + strand at 3241.383 kb on + strand at 3241.383 kb on + strand at 3241.383 kb on + strand at 3241.418 kb on + strand at 3241.418 kb on + strand at 3241.419 kb on - strand at 3241.431 kb on + strand at 3241.444 kb on + strand at 3241.931 kb on + strand, within ABIE53_002868 at 3241.932 kb on - strand, within ABIE53_002868 at 3241.932 kb on - strand, within ABIE53_002868 at 3242.202 kb on + strand at 3242.202 kb on + strand at 3242.203 kb on - strand at 3242.318 kb on + strand, within ABIE53_002869 at 3242.318 kb on + strand, within ABIE53_002869 at 3242.318 kb on + strand, within ABIE53_002869 at 3242.318 kb on + strand, within ABIE53_002869 at 3242.318 kb on + strand, within ABIE53_002869 at 3242.338 kb on - strand, within ABIE53_002869 at 3242.339 kb on + strand, within ABIE53_002869 at 3242.339 kb on + strand, within ABIE53_002869 at 3242.339 kb on + strand, within ABIE53_002869 at 3242.357 kb on + strand, within ABIE53_002869 at 3242.538 kb on - strand, within ABIE53_002869 at 3242.538 kb on - strand, within ABIE53_002869 at 3242.599 kb on - strand, within ABIE53_002869 at 3242.600 kb on + strand, within ABIE53_002869 at 3242.600 kb on + strand, within ABIE53_002869 at 3242.600 kb on + strand, within ABIE53_002869 at 3242.600 kb on + strand, within ABIE53_002869 at 3242.600 kb on + strand, within ABIE53_002869 at 3242.600 kb on + strand, within ABIE53_002869 at 3242.601 kb on - strand, within ABIE53_002869 at 3242.601 kb on - strand, within ABIE53_002869 at 3242.679 kb on - strand, within ABIE53_002869 at 3242.692 kb on + strand, within ABIE53_002869 at 3242.692 kb on + strand, within ABIE53_002869 at 3242.693 kb on - strand, within ABIE53_002869 at 3242.693 kb on - strand, within ABIE53_002869 at 3242.732 kb on + strand, within ABIE53_002869 at 3242.732 kb on + strand, within ABIE53_002869 at 3242.733 kb on - strand, within ABIE53_002869 at 3242.733 kb on - strand, within ABIE53_002869 at 3242.733 kb on - strand, within ABIE53_002869 at 3242.733 kb on - strand, within ABIE53_002869 at 3242.733 kb on - strand, within ABIE53_002869 at 3242.733 kb on - strand, within ABIE53_002869 at 3242.733 kb on - strand, within ABIE53_002869 at 3242.829 kb on + strand, within ABIE53_002869 at 3242.830 kb on - strand, within ABIE53_002869 at 3242.830 kb on - strand, within ABIE53_002869 at 3242.858 kb on + strand at 3242.858 kb on + strand at 3242.858 kb on + strand at 3242.859 kb on - strand at 3242.955 kb on + strand at 3242.956 kb on - strand at 3242.956 kb on - strand at 3242.956 kb on - strand at 3242.976 kb on + strand at 3242.977 kb on - strand at 3242.977 kb on - strand at 3242.977 kb on - strand at 3242.977 kb on - strand at 3242.980 kb on + strand at 3242.980 kb on + strand at 3242.981 kb on - strand at 3243.095 kb on - strand, within ABIE53_002870 at 3243.206 kb on - strand, within ABIE53_002870
Per-strain Table
Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB_gent remove 3,240,480 + ABIE53_002867 0.51 -0.7 3,240,480 + ABIE53_002867 0.51 -1.2 3,240,480 + ABIE53_002867 0.51 -1.4 3,240,480 + ABIE53_002867 0.51 -0.1 3,240,581 + ABIE53_002867 0.56 -1.1 3,240,582 + ABIE53_002867 0.57 -0.2 3,240,582 + ABIE53_002867 0.57 -0.4 3,240,582 + ABIE53_002867 0.57 -0.1 3,240,582 + ABIE53_002867 0.57 -0.1 3,240,583 - ABIE53_002867 0.57 -0.6 3,240,583 - ABIE53_002867 0.57 -0.4 3,240,583 - ABIE53_002867 0.57 -0.7 3,240,583 - ABIE53_002867 0.57 -1.6 3,240,684 + ABIE53_002867 0.62 -1.9 3,240,684 + ABIE53_002867 0.62 +0.3 3,240,684 + ABIE53_002867 0.62 -1.5 3,240,684 + ABIE53_002867 0.62 -0.2 3,240,684 + ABIE53_002867 0.62 -2.5 3,240,684 + ABIE53_002867 0.62 +0.5 3,240,685 - ABIE53_002867 0.63 -1.8 3,240,685 - ABIE53_002867 0.63 -1.3 3,240,685 - ABIE53_002867 0.63 -0.5 3,240,720 + ABIE53_002867 0.65 +1.8 3,240,720 + ABIE53_002867 0.65 +0.4 3,240,720 + ABIE53_002867 0.65 -2.1 3,240,720 + ABIE53_002867 0.65 -0.3 3,240,720 + ABIE53_002867 0.65 -0.2 3,240,720 + ABIE53_002867 0.65 -0.1 3,240,720 + ABIE53_002867 0.65 +3.2 3,240,720 + ABIE53_002867 0.65 -0.1 3,240,720 + ABIE53_002867 0.65 -2.4 3,240,720 + ABIE53_002867 0.65 -0.2 3,240,720 + ABIE53_002867 0.65 -0.1 3,240,721 - ABIE53_002867 0.65 +1.1 3,240,721 - ABIE53_002867 0.65 -0.0 3,240,721 - ABIE53_002867 0.65 +0.4 3,240,721 - ABIE53_002867 0.65 -0.3 3,240,771 + ABIE53_002867 0.68 +4.2 3,240,771 + ABIE53_002867 0.68 -0.2 3,240,772 - ABIE53_002867 0.68 +0.5 3,240,790 - ABIE53_002867 0.69 -1.9 3,240,909 + ABIE53_002867 0.76 -0.0 3,240,909 + ABIE53_002867 0.76 +0.4 3,240,909 + ABIE53_002867 0.76 +1.7 3,240,910 - ABIE53_002867 0.76 -1.4 3,240,910 - ABIE53_002867 0.76 -1.0 3,240,910 - ABIE53_002867 0.76 -2.2 3,240,910 - ABIE53_002867 0.76 +1.8 3,240,910 - ABIE53_002867 0.76 -0.6 3,240,910 - ABIE53_002867 0.76 -0.2 3,240,936 + ABIE53_002867 0.77 -0.3 3,240,936 + ABIE53_002867 0.77 -0.7 3,240,936 + ABIE53_002867 0.77 -0.2 3,240,936 + ABIE53_002867 0.77 +0.5 3,240,936 + ABIE53_002867 0.77 -0.8 3,240,936 + ABIE53_002867 0.77 +1.4 3,240,936 + ABIE53_002867 0.77 -2.4 3,240,948 - ABIE53_002867 0.78 +2.6 3,240,977 + ABIE53_002867 0.80 +0.3 3,240,977 + ABIE53_002867 0.80 +0.6 3,240,977 + ABIE53_002867 0.80 +2.4 3,240,978 - ABIE53_002867 0.80 +0.2 3,241,089 + ABIE53_002867 0.86 +2.2 3,241,225 + +0.3 3,241,248 + -2.0 3,241,248 + +0.6 3,241,248 + +0.2 3,241,249 - -0.8 3,241,249 - +0.3 3,241,326 + -3.0 3,241,326 + -3.6 3,241,326 + -0.3 3,241,326 + +2.2 3,241,327 - +1.3 3,241,327 - +0.2 3,241,327 - +0.8 3,241,327 - -0.0 3,241,337 + +1.3 3,241,337 + +0.8 3,241,337 + +1.7 3,241,383 + +0.5 3,241,383 + -2.4 3,241,383 + -1.5 3,241,418 + -0.0 3,241,418 + +0.1 3,241,419 - -1.2 3,241,431 + -0.2 3,241,444 + +0.2 3,241,931 + ABIE53_002868 0.62 +3.2 3,241,932 - ABIE53_002868 0.62 -0.2 3,241,932 - ABIE53_002868 0.62 -1.0 3,242,202 + -2.1 3,242,202 + +0.6 3,242,203 - -1.5 3,242,318 + ABIE53_002869 0.13 -1.0 3,242,318 + ABIE53_002869 0.13 +0.1 3,242,318 + ABIE53_002869 0.13 -3.0 3,242,318 + ABIE53_002869 0.13 -0.1 3,242,318 + ABIE53_002869 0.13 +2.3 3,242,338 - ABIE53_002869 0.16 -1.9 3,242,339 + ABIE53_002869 0.16 +0.9 3,242,339 + ABIE53_002869 0.16 -0.3 3,242,339 + ABIE53_002869 0.16 -0.3 3,242,357 + ABIE53_002869 0.19 +5.0 3,242,538 - ABIE53_002869 0.45 -0.5 3,242,538 - ABIE53_002869 0.45 -0.4 3,242,599 - ABIE53_002869 0.54 +1.1 3,242,600 + ABIE53_002869 0.55 -0.3 3,242,600 + ABIE53_002869 0.55 +0.4 3,242,600 + ABIE53_002869 0.55 +0.0 3,242,600 + ABIE53_002869 0.55 -0.0 3,242,600 + ABIE53_002869 0.55 +1.2 3,242,600 + ABIE53_002869 0.55 -1.2 3,242,601 - ABIE53_002869 0.55 +0.8 3,242,601 - ABIE53_002869 0.55 -1.0 3,242,679 - ABIE53_002869 0.66 +0.3 3,242,692 + ABIE53_002869 0.68 -0.3 3,242,692 + ABIE53_002869 0.68 +0.1 3,242,693 - ABIE53_002869 0.68 -0.0 3,242,693 - ABIE53_002869 0.68 -0.7 3,242,732 + ABIE53_002869 0.74 -0.9 3,242,732 + ABIE53_002869 0.74 -0.4 3,242,733 - ABIE53_002869 0.74 -1.3 3,242,733 - ABIE53_002869 0.74 -0.2 3,242,733 - ABIE53_002869 0.74 -0.6 3,242,733 - ABIE53_002869 0.74 +0.7 3,242,733 - ABIE53_002869 0.74 +1.0 3,242,733 - ABIE53_002869 0.74 -0.8 3,242,733 - ABIE53_002869 0.74 -1.0 3,242,829 + ABIE53_002869 0.88 -1.2 3,242,830 - ABIE53_002869 0.88 -1.3 3,242,830 - ABIE53_002869 0.88 -0.1 3,242,858 + +0.0 3,242,858 + -0.3 3,242,858 + -1.3 3,242,859 - +0.5 3,242,955 + -0.6 3,242,956 - -1.2 3,242,956 - -1.4 3,242,956 - -1.3 3,242,976 + -0.2 3,242,977 - +0.7 3,242,977 - +1.8 3,242,977 - +0.7 3,242,977 - -0.3 3,242,980 + +1.6 3,242,980 + -0.4 3,242,981 - +2.2 3,243,095 - ABIE53_002870 0.21 -0.0 3,243,206 - ABIE53_002870 0.35 +1.5
Or see this region's nucleotide sequence