Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS14425

Experiment: cage3; mouse2; day3; diet=PolysaccharideDeficient; sample=FecalPellet; coculture=Btheta_VPI5482; stagger=24hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBBR_RS14415 and BBR_RS14420 are separated by 3 nucleotidesBBR_RS14420 and BBR_RS14425 overlap by 4 nucleotidesBBR_RS14425 and BBR_RS14430 are separated by 127 nucleotidesBBR_RS14430 and BBR_RS14435 are separated by 83 nucleotidesBBR_RS14435 and BBR_RS14440 are separated by 47 nucleotides BBR_RS14415: BBR_RS14415 - bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU, at 1,010,467 to 1,011,849 _RS14415 BBR_RS14420: BBR_RS14420 - ribosome silencing factor, at 1,011,853 to 1,012,239 _RS14420 BBR_RS14425: BBR_RS14425 - histidine phosphatase family protein, at 1,012,236 to 1,012,937 _RS14425 BBR_RS14430: BBR_RS14430 - tRNA-Ala, at 1,013,065 to 1,013,140 _RS14430 BBR_RS14435: BBR_RS14435 - tRNA-Ala, at 1,013,224 to 1,013,299 _RS14435 BBR_RS14440: BBR_RS14440 - Cof-type HAD-IIB family hydrolase, at 1,013,347 to 1,014,156 _RS14440 Position (kb) 1012 1013Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1011.843 kb on + strandat 1011.870 kb on - strandat 1011.911 kb on + strand, within BBR_RS14420at 1011.919 kb on - strand, within BBR_RS14420at 1012.089 kb on - strand, within BBR_RS14420at 1012.089 kb on - strand, within BBR_RS14420at 1012.090 kb on + strand, within BBR_RS14420at 1012.090 kb on - strand, within BBR_RS14420at 1012.100 kb on + strand, within BBR_RS14420at 1012.106 kb on + strand, within BBR_RS14420at 1012.156 kb on + strand, within BBR_RS14420at 1012.166 kb on + strand, within BBR_RS14420at 1012.173 kb on - strand, within BBR_RS14420at 1012.279 kb on + strandat 1012.279 kb on + strandat 1012.282 kb on - strandat 1012.289 kb on + strandat 1012.297 kb on - strandat 1012.297 kb on - strandat 1012.317 kb on + strand, within BBR_RS14425at 1012.317 kb on + strand, within BBR_RS14425at 1012.334 kb on + strand, within BBR_RS14425at 1012.352 kb on - strand, within BBR_RS14425at 1012.358 kb on + strand, within BBR_RS14425at 1012.358 kb on + strand, within BBR_RS14425at 1012.358 kb on + strand, within BBR_RS14425at 1012.385 kb on + strand, within BBR_RS14425at 1012.432 kb on + strand, within BBR_RS14425at 1012.437 kb on + strand, within BBR_RS14425at 1012.437 kb on + strand, within BBR_RS14425at 1012.440 kb on - strand, within BBR_RS14425at 1012.468 kb on + strand, within BBR_RS14425at 1012.661 kb on - strand, within BBR_RS14425at 1012.670 kb on + strand, within BBR_RS14425at 1012.724 kb on + strand, within BBR_RS14425at 1012.724 kb on + strand, within BBR_RS14425at 1012.751 kb on - strand, within BBR_RS14425at 1012.752 kb on + strand, within BBR_RS14425at 1012.760 kb on - strand, within BBR_RS14425at 1012.799 kb on + strand, within BBR_RS14425at 1012.821 kb on + strand, within BBR_RS14425at 1012.824 kb on - strand, within BBR_RS14425at 1012.840 kb on + strand, within BBR_RS14425at 1012.849 kb on + strand, within BBR_RS14425at 1012.851 kb on + strand, within BBR_RS14425at 1012.883 kb on + strandat 1012.894 kb on + strandat 1012.909 kb on + strandat 1012.927 kb on - strandat 1012.936 kb on + strandat 1013.345 kb on + strandat 1013.447 kb on + strand, within BBR_RS14440at 1013.468 kb on + strand, within BBR_RS14440at 1013.474 kb on + strand, within BBR_RS14440at 1013.526 kb on + strand, within BBR_RS14440at 1013.548 kb on - strand, within BBR_RS14440at 1013.566 kb on + strand, within BBR_RS14440at 1013.566 kb on + strand, within BBR_RS14440at 1013.588 kb on + strand, within BBR_RS14440at 1013.593 kb on + strand, within BBR_RS14440at 1013.646 kb on + strand, within BBR_RS14440at 1013.789 kb on - strand, within BBR_RS14440at 1013.799 kb on + strand, within BBR_RS14440at 1013.888 kb on - strand, within BBR_RS14440at 1013.897 kb on - strand, within BBR_RS14440at 1013.897 kb on - strand, within BBR_RS14440at 1013.930 kb on - strand, within BBR_RS14440

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Per-strain Table

Position Strand Gene LocusTag Fraction cage3; mouse2; day3; diet=PolysaccharideDeficient; sample=FecalPellet; coculture=Btheta_VPI5482; stagger=24hrs
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1,011,843 + +1.3
1,011,870 - -0.2
1,011,911 + BBR_RS14420 0.15 +0.1
1,011,919 - BBR_RS14420 0.17 -1.6
1,012,089 - BBR_RS14420 0.61 -0.5
1,012,089 - BBR_RS14420 0.61 -0.2
1,012,090 + BBR_RS14420 0.61 -1.6
1,012,090 - BBR_RS14420 0.61 +1.5
1,012,100 + BBR_RS14420 0.64 -0.2
1,012,106 + BBR_RS14420 0.65 -2.0
1,012,156 + BBR_RS14420 0.78 +0.3
1,012,166 + BBR_RS14420 0.81 -1.1
1,012,173 - BBR_RS14420 0.83 -1.8
1,012,279 + -0.2
1,012,279 + +0.2
1,012,282 - -0.6
1,012,289 + -0.8
1,012,297 - -0.4
1,012,297 - +0.4
1,012,317 + BBR_RS14425 0.12 -1.2
1,012,317 + BBR_RS14425 0.12 -0.3
1,012,334 + BBR_RS14425 0.14 -0.1
1,012,352 - BBR_RS14425 0.17 -0.4
1,012,358 + BBR_RS14425 0.17 +1.2
1,012,358 + BBR_RS14425 0.17 +0.1
1,012,358 + BBR_RS14425 0.17 +0.2
1,012,385 + BBR_RS14425 0.21 +2.9
1,012,432 + BBR_RS14425 0.28 +0.1
1,012,437 + BBR_RS14425 0.29 -0.9
1,012,437 + BBR_RS14425 0.29 +0.4
1,012,440 - BBR_RS14425 0.29 +0.4
1,012,468 + BBR_RS14425 0.33 -0.3
1,012,661 - BBR_RS14425 0.61 -0.4
1,012,670 + BBR_RS14425 0.62 -0.5
1,012,724 + BBR_RS14425 0.70 +0.7
1,012,724 + BBR_RS14425 0.70 -0.4
1,012,751 - BBR_RS14425 0.73 +1.0
1,012,752 + BBR_RS14425 0.74 -0.5
1,012,760 - BBR_RS14425 0.75 +1.2
1,012,799 + BBR_RS14425 0.80 -0.9
1,012,821 + BBR_RS14425 0.83 +1.1
1,012,824 - BBR_RS14425 0.84 -0.3
1,012,840 + BBR_RS14425 0.86 -0.4
1,012,849 + BBR_RS14425 0.87 +0.2
1,012,851 + BBR_RS14425 0.88 -1.0
1,012,883 + -0.6
1,012,894 + -0.5
1,012,909 + +0.1
1,012,927 - -0.3
1,012,936 + -0.4
1,013,345 + -1.3
1,013,447 + BBR_RS14440 0.12 -1.7
1,013,468 + BBR_RS14440 0.15 +0.5
1,013,474 + BBR_RS14440 0.16 +0.3
1,013,526 + BBR_RS14440 0.22 +2.5
1,013,548 - BBR_RS14440 0.25 +0.2
1,013,566 + BBR_RS14440 0.27 +0.0
1,013,566 + BBR_RS14440 0.27 -0.8
1,013,588 + BBR_RS14440 0.30 -0.2
1,013,593 + BBR_RS14440 0.30 +0.3
1,013,646 + BBR_RS14440 0.37 +0.2
1,013,789 - BBR_RS14440 0.55 -1.1
1,013,799 + BBR_RS14440 0.56 +0.0
1,013,888 - BBR_RS14440 0.67 -0.3
1,013,897 - BBR_RS14440 0.68 +0.5
1,013,897 - BBR_RS14440 0.68 -0.5
1,013,930 - BBR_RS14440 0.72 +1.1

Or see this region's nucleotide sequence