Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS14340

Experiment: cage3; mouse1; day3; diet=PolysaccharideDeficient; sample=FecalPellet; coculture=Btheta_VPI5482; stagger=24hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBBR_RS14330 and BBR_RS14335 are separated by 137 nucleotidesBBR_RS14335 and BBR_RS14340 are separated by 148 nucleotidesBBR_RS14340 and BBR_RS14345 are separated by 3 nucleotidesBBR_RS14345 and BBR_RS14350 are separated by 168 nucleotides BBR_RS14330: BBR_RS14330 - glycogen debranching enzyme GlgX, at 990,756 to 992,867 _RS14330 BBR_RS14335: BBR_RS14335 - hypothetical protein, at 993,005 to 993,205 _RS14335 BBR_RS14340: BBR_RS14340 - helix-turn-helix domain-containing protein, at 993,354 to 993,839 _RS14340 BBR_RS14345: BBR_RS14345 - hypothetical protein, at 993,843 to 994,493 _RS14345 BBR_RS14350: BBR_RS14350 - hypothetical protein, at 994,662 to 994,889 _RS14350 Position (kb) 993 994Strain fitness (log2 ratio) -2 -1 0 1 2 3at 992.546 kb on - strand, within BBR_RS14330at 992.573 kb on + strand, within BBR_RS14330at 992.678 kb on + strandat 992.788 kb on + strandat 992.910 kb on + strandat 992.917 kb on + strandat 992.917 kb on + strandat 992.956 kb on - strandat 992.962 kb on + strandat 993.000 kb on + strandat 993.101 kb on + strand, within BBR_RS14335at 993.101 kb on + strand, within BBR_RS14335at 993.125 kb on - strand, within BBR_RS14335at 993.132 kb on + strand, within BBR_RS14335at 993.132 kb on + strand, within BBR_RS14335at 993.140 kb on - strand, within BBR_RS14335at 993.181 kb on + strand, within BBR_RS14335at 993.189 kb on - strandat 993.286 kb on + strandat 993.294 kb on - strandat 993.304 kb on - strandat 993.371 kb on + strandat 993.390 kb on + strandat 993.519 kb on - strand, within BBR_RS14340at 993.732 kb on + strand, within BBR_RS14340at 993.742 kb on + strand, within BBR_RS14340at 993.760 kb on - strand, within BBR_RS14340at 993.760 kb on - strand, within BBR_RS14340at 993.809 kb on - strandat 993.874 kb on - strandat 993.943 kb on + strand, within BBR_RS14345at 993.943 kb on + strand, within BBR_RS14345at 993.956 kb on + strand, within BBR_RS14345at 993.985 kb on - strand, within BBR_RS14345at 993.995 kb on + strand, within BBR_RS14345at 994.003 kb on - strand, within BBR_RS14345at 994.003 kb on - strand, within BBR_RS14345at 994.088 kb on + strand, within BBR_RS14345at 994.095 kb on + strand, within BBR_RS14345at 994.095 kb on + strand, within BBR_RS14345at 994.105 kb on + strand, within BBR_RS14345at 994.113 kb on - strand, within BBR_RS14345at 994.219 kb on - strand, within BBR_RS14345at 994.266 kb on - strand, within BBR_RS14345at 994.283 kb on + strand, within BBR_RS14345at 994.298 kb on + strand, within BBR_RS14345at 994.328 kb on + strand, within BBR_RS14345at 994.386 kb on - strand, within BBR_RS14345at 994.428 kb on - strand, within BBR_RS14345at 994.507 kb on + strandat 994.510 kb on - strandat 994.606 kb on + strandat 994.614 kb on - strandat 994.614 kb on - strandat 994.702 kb on - strand, within BBR_RS14350at 994.724 kb on - strand, within BBR_RS14350at 994.729 kb on + strand, within BBR_RS14350at 994.747 kb on - strand, within BBR_RS14350at 994.770 kb on + strand, within BBR_RS14350at 994.794 kb on + strand, within BBR_RS14350

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Per-strain Table

Position Strand Gene LocusTag Fraction cage3; mouse1; day3; diet=PolysaccharideDeficient; sample=FecalPellet; coculture=Btheta_VPI5482; stagger=24hrs
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992,546 - BBR_RS14330 0.85 +0.8
992,573 + BBR_RS14330 0.86 -1.2
992,678 + +2.9
992,788 + +1.4
992,910 + -0.3
992,917 + +0.2
992,917 + -0.1
992,956 - +0.3
992,962 + +0.9
993,000 + +0.4
993,101 + BBR_RS14335 0.48 +0.7
993,101 + BBR_RS14335 0.48 +0.6
993,125 - BBR_RS14335 0.60 -0.8
993,132 + BBR_RS14335 0.63 -0.3
993,132 + BBR_RS14335 0.63 +1.3
993,140 - BBR_RS14335 0.67 -0.4
993,181 + BBR_RS14335 0.88 +0.9
993,189 - +1.1
993,286 + +0.0
993,294 - -1.7
993,304 - -0.1
993,371 + -0.2
993,390 + +0.2
993,519 - BBR_RS14340 0.34 -0.8
993,732 + BBR_RS14340 0.78 -0.8
993,742 + BBR_RS14340 0.80 -0.9
993,760 - BBR_RS14340 0.84 -0.7
993,760 - BBR_RS14340 0.84 -0.3
993,809 - +0.5
993,874 - -0.1
993,943 + BBR_RS14345 0.15 +1.5
993,943 + BBR_RS14345 0.15 -1.0
993,956 + BBR_RS14345 0.17 -0.1
993,985 - BBR_RS14345 0.22 +2.0
993,995 + BBR_RS14345 0.23 +0.4
994,003 - BBR_RS14345 0.25 -0.3
994,003 - BBR_RS14345 0.25 +0.1
994,088 + BBR_RS14345 0.38 -0.5
994,095 + BBR_RS14345 0.39 -2.5
994,095 + BBR_RS14345 0.39 +0.1
994,105 + BBR_RS14345 0.40 -1.5
994,113 - BBR_RS14345 0.41 +0.1
994,219 - BBR_RS14345 0.58 +0.1
994,266 - BBR_RS14345 0.65 +0.2
994,283 + BBR_RS14345 0.68 +1.3
994,298 + BBR_RS14345 0.70 +0.1
994,328 + BBR_RS14345 0.75 -0.9
994,386 - BBR_RS14345 0.83 -0.4
994,428 - BBR_RS14345 0.90 +0.3
994,507 + +0.1
994,510 - -1.2
994,606 + -1.7
994,614 - +0.5
994,614 - -0.7
994,702 - BBR_RS14350 0.18 +0.6
994,724 - BBR_RS14350 0.27 +0.5
994,729 + BBR_RS14350 0.29 -1.4
994,747 - BBR_RS14350 0.37 +0.9
994,770 + BBR_RS14350 0.47 -0.6
994,794 + BBR_RS14350 0.58 +1.0

Or see this region's nucleotide sequence