Experiment: Glucose (C) and Ammonium chloride (N); with MOPS; with PEG
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS03635 and PFLU_RS03640 are separated by 1 nucleotides PFLU_RS03640 and PFLU_RS03645 are separated by 4 nucleotides PFLU_RS03645 and PFLU_RS03650 are separated by 182 nucleotides
PFLU_RS03635: PFLU_RS03635 - 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, at 834,374 to 835,225
_RS03635
PFLU_RS03640: PFLU_RS03640 - lipoprotein localization protein LolB, at 835,227 to 835,844
_RS03640
PFLU_RS03645: PFLU_RS03645 - tetratricopeptide repeat protein, at 835,849 to 837,573
_RS03645
PFLU_RS03650: PFLU_RS03650 - glutamyl-tRNA reductase, at 837,756 to 839,045
_RS03650
Position (kb) 835
836
837
838 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 835.845 kb on - strand at 835.845 kb on - strand at 835.845 kb on - strand at 835.845 kb on - strand at 835.845 kb on - strand at 835.845 kb on - strand at 835.845 kb on - strand at 835.845 kb on - strand at 835.845 kb on - strand at 835.845 kb on - strand at 835.845 kb on - strand at 835.845 kb on - strand at 835.845 kb on - strand at 835.845 kb on - strand at 835.845 kb on - strand at 835.850 kb on + strand at 835.851 kb on - strand at 835.851 kb on - strand at 835.851 kb on - strand at 835.851 kb on - strand at 836.066 kb on + strand, within PFLU_RS03645 at 836.067 kb on - strand, within PFLU_RS03645 at 836.153 kb on + strand, within PFLU_RS03645 at 836.180 kb on + strand, within PFLU_RS03645 at 836.180 kb on + strand, within PFLU_RS03645 at 836.181 kb on - strand, within PFLU_RS03645 at 836.294 kb on + strand, within PFLU_RS03645 at 836.294 kb on + strand, within PFLU_RS03645 at 836.295 kb on - strand, within PFLU_RS03645 at 836.295 kb on - strand, within PFLU_RS03645 at 836.295 kb on - strand, within PFLU_RS03645 at 836.400 kb on - strand, within PFLU_RS03645 at 836.400 kb on - strand, within PFLU_RS03645 at 836.400 kb on - strand, within PFLU_RS03645 at 836.400 kb on - strand, within PFLU_RS03645 at 836.400 kb on - strand, within PFLU_RS03645 at 836.414 kb on + strand, within PFLU_RS03645 at 836.517 kb on - strand, within PFLU_RS03645 at 836.517 kb on - strand, within PFLU_RS03645 at 836.517 kb on - strand, within PFLU_RS03645 at 836.544 kb on - strand, within PFLU_RS03645 at 836.544 kb on - strand, within PFLU_RS03645 at 836.649 kb on - strand, within PFLU_RS03645 at 836.686 kb on + strand, within PFLU_RS03645 at 836.686 kb on + strand, within PFLU_RS03645 at 836.686 kb on + strand, within PFLU_RS03645 at 836.686 kb on + strand, within PFLU_RS03645 at 836.686 kb on + strand, within PFLU_RS03645 at 836.686 kb on + strand, within PFLU_RS03645 at 836.686 kb on + strand, within PFLU_RS03645 at 836.686 kb on + strand, within PFLU_RS03645 at 836.686 kb on + strand, within PFLU_RS03645 at 836.687 kb on - strand, within PFLU_RS03645 at 836.687 kb on - strand, within PFLU_RS03645 at 836.687 kb on - strand, within PFLU_RS03645 at 836.687 kb on - strand, within PFLU_RS03645 at 836.687 kb on - strand, within PFLU_RS03645 at 836.687 kb on - strand, within PFLU_RS03645 at 836.687 kb on - strand, within PFLU_RS03645 at 836.799 kb on - strand, within PFLU_RS03645 at 836.799 kb on - strand, within PFLU_RS03645 at 836.799 kb on - strand, within PFLU_RS03645 at 837.153 kb on + strand, within PFLU_RS03645 at 837.154 kb on - strand, within PFLU_RS03645 at 837.154 kb on - strand, within PFLU_RS03645 at 837.154 kb on - strand, within PFLU_RS03645 at 837.156 kb on - strand, within PFLU_RS03645 at 837.156 kb on - strand, within PFLU_RS03645 at 837.156 kb on - strand, within PFLU_RS03645 at 837.156 kb on - strand, within PFLU_RS03645 at 837.156 kb on - strand, within PFLU_RS03645 at 837.156 kb on - strand, within PFLU_RS03645 at 837.156 kb on - strand, within PFLU_RS03645 at 837.156 kb on - strand, within PFLU_RS03645 at 837.156 kb on - strand, within PFLU_RS03645 at 837.156 kb on - strand, within PFLU_RS03645 at 837.156 kb on - strand, within PFLU_RS03645 at 837.156 kb on - strand, within PFLU_RS03645 at 837.156 kb on - strand, within PFLU_RS03645 at 837.156 kb on - strand, within PFLU_RS03645 at 837.156 kb on - strand, within PFLU_RS03645 at 837.287 kb on + strand, within PFLU_RS03645 at 837.288 kb on - strand, within PFLU_RS03645 at 837.288 kb on - strand, within PFLU_RS03645 at 837.288 kb on - strand, within PFLU_RS03645 at 837.288 kb on - strand, within PFLU_RS03645 at 837.353 kb on + strand, within PFLU_RS03645 at 837.353 kb on + strand, within PFLU_RS03645 at 837.353 kb on + strand, within PFLU_RS03645 at 837.353 kb on + strand, within PFLU_RS03645 at 837.353 kb on + strand, within PFLU_RS03645 at 837.353 kb on + strand, within PFLU_RS03645 at 837.354 kb on - strand, within PFLU_RS03645 at 837.354 kb on - strand, within PFLU_RS03645 at 837.354 kb on - strand, within PFLU_RS03645 at 837.354 kb on - strand, within PFLU_RS03645 at 837.354 kb on - strand, within PFLU_RS03645 at 837.354 kb on - strand, within PFLU_RS03645 at 837.354 kb on - strand, within PFLU_RS03645 at 837.354 kb on - strand, within PFLU_RS03645 at 837.354 kb on - strand, within PFLU_RS03645 at 837.354 kb on - strand, within PFLU_RS03645 at 837.354 kb on - strand, within PFLU_RS03645 at 837.354 kb on - strand, within PFLU_RS03645 at 837.354 kb on - strand, within PFLU_RS03645 at 837.358 kb on - strand, within PFLU_RS03645 at 837.358 kb on - strand, within PFLU_RS03645 at 837.358 kb on - strand, within PFLU_RS03645 at 837.358 kb on - strand, within PFLU_RS03645 at 837.416 kb on + strand at 837.416 kb on + strand at 837.416 kb on + strand at 837.417 kb on - strand at 837.444 kb on - strand at 837.444 kb on - strand at 837.444 kb on - strand at 837.568 kb on - strand at 837.568 kb on - strand at 837.568 kb on - strand at 837.568 kb on - strand at 837.568 kb on - strand at 837.568 kb on - strand at 837.568 kb on - strand at 837.568 kb on - strand at 837.568 kb on - strand at 837.568 kb on - strand at 837.568 kb on - strand at 837.568 kb on - strand at 837.568 kb on - strand at 837.568 kb on - strand at 837.568 kb on - strand at 837.568 kb on - strand at 837.568 kb on - strand at 837.568 kb on - strand at 837.568 kb on - strand at 837.568 kb on - strand at 837.568 kb on - strand at 837.568 kb on - strand at 837.568 kb on - strand at 837.568 kb on - strand at 837.568 kb on - strand at 837.568 kb on - strand at 837.568 kb on - strand at 837.573 kb on + strand at 837.573 kb on + strand at 837.573 kb on + strand at 837.573 kb on + strand at 837.573 kb on + strand at 837.574 kb on - strand at 837.574 kb on - strand at 837.574 kb on - strand at 837.574 kb on - strand at 837.574 kb on - strand at 837.575 kb on + strand at 837.575 kb on + strand at 837.575 kb on + strand at 837.575 kb on + strand at 837.575 kb on + strand at 837.575 kb on + strand at 837.575 kb on + strand at 837.575 kb on + strand at 837.575 kb on + strand at 837.575 kb on + strand at 837.575 kb on + strand at 837.575 kb on + strand at 837.575 kb on + strand at 837.575 kb on + strand at 837.575 kb on + strand at 837.575 kb on + strand at 837.575 kb on + strand at 837.576 kb on - strand at 837.576 kb on - strand at 837.576 kb on - strand at 837.576 kb on - strand at 837.576 kb on - strand at 837.576 kb on - strand at 837.576 kb on - strand at 837.576 kb on - strand at 837.576 kb on - strand at 837.576 kb on - strand at 837.576 kb on - strand at 837.576 kb on - strand at 837.576 kb on - strand at 837.576 kb on - strand at 837.576 kb on - strand at 837.576 kb on - strand at 837.576 kb on - strand at 837.576 kb on - strand at 837.576 kb on - strand at 837.576 kb on - strand at 837.576 kb on - strand at 837.576 kb on - strand at 837.576 kb on - strand at 837.634 kb on + strand at 837.634 kb on + strand at 837.634 kb on + strand at 837.634 kb on + strand at 837.634 kb on + strand at 837.634 kb on + strand at 837.635 kb on - strand at 837.635 kb on - strand at 837.635 kb on - strand at 837.635 kb on - strand at 837.635 kb on - strand at 837.635 kb on - strand at 837.658 kb on + strand at 837.658 kb on + strand at 837.658 kb on + strand at 837.658 kb on + strand at 837.658 kb on + strand at 837.658 kb on + strand at 837.658 kb on + strand at 837.658 kb on + strand at 837.659 kb on - strand at 837.659 kb on - strand at 837.660 kb on + strand at 837.660 kb on + strand at 837.661 kb on - strand at 837.661 kb on - strand at 837.661 kb on - strand at 837.661 kb on - strand at 837.661 kb on - strand at 837.674 kb on + strand at 837.674 kb on + strand at 837.675 kb on - strand at 837.682 kb on + strand at 837.682 kb on + strand at 837.682 kb on + strand at 837.683 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glucose (C) and Ammonium chloride (N); with MOPS; with PEG remove 835,845 - +1.0 835,845 - -1.3 835,845 - -1.4 835,845 - +1.4 835,845 - -1.0 835,845 - +0.3 835,845 - +0.7 835,845 - +0.0 835,845 - -0.4 835,845 - -0.6 835,845 - +0.9 835,845 - -0.4 835,845 - -1.1 835,845 - +0.8 835,845 - +2.2 835,850 + +3.2 835,851 - +1.0 835,851 - +0.5 835,851 - -1.1 835,851 - +0.5 836,066 + PFLU_RS03645 0.13 -3.1 836,067 - PFLU_RS03645 0.13 -0.3 836,153 + PFLU_RS03645 0.18 -0.5 836,180 + PFLU_RS03645 0.19 +0.6 836,180 + PFLU_RS03645 0.19 -0.6 836,181 - PFLU_RS03645 0.19 -0.6 836,294 + PFLU_RS03645 0.26 +2.8 836,294 + PFLU_RS03645 0.26 +1.2 836,295 - PFLU_RS03645 0.26 -1.9 836,295 - PFLU_RS03645 0.26 +0.6 836,295 - PFLU_RS03645 0.26 -0.1 836,400 - PFLU_RS03645 0.32 -2.1 836,400 - PFLU_RS03645 0.32 +0.9 836,400 - PFLU_RS03645 0.32 +0.1 836,400 - PFLU_RS03645 0.32 -2.1 836,400 - PFLU_RS03645 0.32 -0.1 836,414 + PFLU_RS03645 0.33 +2.2 836,517 - PFLU_RS03645 0.39 -2.6 836,517 - PFLU_RS03645 0.39 -1.1 836,517 - PFLU_RS03645 0.39 -2.0 836,544 - PFLU_RS03645 0.40 -0.2 836,544 - PFLU_RS03645 0.40 +1.7 836,649 - PFLU_RS03645 0.46 -1.1 836,686 + PFLU_RS03645 0.49 -1.8 836,686 + PFLU_RS03645 0.49 -1.4 836,686 + PFLU_RS03645 0.49 -0.1 836,686 + PFLU_RS03645 0.49 +2.2 836,686 + PFLU_RS03645 0.49 +0.6 836,686 + PFLU_RS03645 0.49 -0.4 836,686 + PFLU_RS03645 0.49 -1.3 836,686 + PFLU_RS03645 0.49 +0.6 836,686 + PFLU_RS03645 0.49 -1.5 836,687 - PFLU_RS03645 0.49 -0.8 836,687 - PFLU_RS03645 0.49 -1.2 836,687 - PFLU_RS03645 0.49 -1.7 836,687 - PFLU_RS03645 0.49 -0.3 836,687 - PFLU_RS03645 0.49 +3.8 836,687 - PFLU_RS03645 0.49 -2.6 836,687 - PFLU_RS03645 0.49 -0.4 836,799 - PFLU_RS03645 0.55 +0.2 836,799 - PFLU_RS03645 0.55 -0.2 836,799 - PFLU_RS03645 0.55 -1.8 837,153 + PFLU_RS03645 0.76 +1.2 837,154 - PFLU_RS03645 0.76 +0.4 837,154 - PFLU_RS03645 0.76 -1.0 837,154 - PFLU_RS03645 0.76 -1.1 837,156 - PFLU_RS03645 0.76 -1.7 837,156 - PFLU_RS03645 0.76 -1.2 837,156 - PFLU_RS03645 0.76 -0.1 837,156 - PFLU_RS03645 0.76 -3.4 837,156 - PFLU_RS03645 0.76 +1.0 837,156 - PFLU_RS03645 0.76 +0.2 837,156 - PFLU_RS03645 0.76 -2.2 837,156 - PFLU_RS03645 0.76 -0.2 837,156 - PFLU_RS03645 0.76 -0.1 837,156 - PFLU_RS03645 0.76 -2.3 837,156 - PFLU_RS03645 0.76 +0.8 837,156 - PFLU_RS03645 0.76 +0.6 837,156 - PFLU_RS03645 0.76 -0.5 837,156 - PFLU_RS03645 0.76 +0.3 837,156 - PFLU_RS03645 0.76 -0.3 837,287 + PFLU_RS03645 0.83 +0.6 837,288 - PFLU_RS03645 0.83 -0.4 837,288 - PFLU_RS03645 0.83 -0.1 837,288 - PFLU_RS03645 0.83 -1.7 837,288 - PFLU_RS03645 0.83 -2.0 837,353 + PFLU_RS03645 0.87 +1.2 837,353 + PFLU_RS03645 0.87 -0.4 837,353 + PFLU_RS03645 0.87 -0.1 837,353 + PFLU_RS03645 0.87 +3.2 837,353 + PFLU_RS03645 0.87 +0.6 837,353 + PFLU_RS03645 0.87 +2.2 837,354 - PFLU_RS03645 0.87 +0.9 837,354 - PFLU_RS03645 0.87 +0.3 837,354 - PFLU_RS03645 0.87 +1.0 837,354 - PFLU_RS03645 0.87 -0.7 837,354 - PFLU_RS03645 0.87 -0.9 837,354 - PFLU_RS03645 0.87 +0.0 837,354 - PFLU_RS03645 0.87 -0.9 837,354 - PFLU_RS03645 0.87 -0.2 837,354 - PFLU_RS03645 0.87 -2.1 837,354 - PFLU_RS03645 0.87 -0.1 837,354 - PFLU_RS03645 0.87 -1.7 837,354 - PFLU_RS03645 0.87 -0.6 837,354 - PFLU_RS03645 0.87 -0.1 837,358 - PFLU_RS03645 0.87 -1.9 837,358 - PFLU_RS03645 0.87 +0.1 837,358 - PFLU_RS03645 0.87 -0.1 837,358 - PFLU_RS03645 0.87 -0.8 837,416 + +1.2 837,416 + -1.4 837,416 + +2.2 837,417 - -0.1 837,444 - -2.1 837,444 - -1.2 837,444 - -1.1 837,568 - +0.8 837,568 - +1.2 837,568 - -1.0 837,568 - -0.4 837,568 - +0.2 837,568 - -1.7 837,568 - +0.6 837,568 - +1.6 837,568 - +1.9 837,568 - -2.1 837,568 - +0.6 837,568 - -1.4 837,568 - +1.2 837,568 - +2.2 837,568 - -0.4 837,568 - -0.6 837,568 - -0.6 837,568 - +0.2 837,568 - -0.8 837,568 - +0.6 837,568 - +2.2 837,568 - -0.9 837,568 - +0.6 837,568 - -0.8 837,568 - +2.2 837,568 - -1.1 837,568 - -0.8 837,573 + +0.3 837,573 + -1.3 837,573 + +1.2 837,573 + -1.1 837,573 + -0.8 837,574 - +2.5 837,574 - -0.7 837,574 - +0.7 837,574 - +0.6 837,574 - +0.1 837,575 + -0.7 837,575 + -1.0 837,575 + +0.2 837,575 + -0.5 837,575 + +0.2 837,575 + +1.0 837,575 + -2.5 837,575 + -0.4 837,575 + -1.8 837,575 + +2.2 837,575 + -2.4 837,575 + +0.7 837,575 + +0.3 837,575 + -2.3 837,575 + -0.8 837,575 + -0.4 837,575 + -0.8 837,576 - +0.5 837,576 - -0.9 837,576 - -0.2 837,576 - -2.2 837,576 - +1.9 837,576 - +1.4 837,576 - -1.0 837,576 - +0.2 837,576 - -0.7 837,576 - -0.1 837,576 - +0.6 837,576 - +0.5 837,576 - -0.3 837,576 - +0.4 837,576 - -0.5 837,576 - +0.9 837,576 - +0.9 837,576 - -2.6 837,576 - +0.4 837,576 - -0.1 837,576 - +1.2 837,576 - +0.4 837,576 - -0.2 837,634 + -0.9 837,634 + -0.3 837,634 + -1.0 837,634 + +0.5 837,634 + +0.9 837,634 + +0.2 837,635 - +0.6 837,635 - +1.1 837,635 - -0.4 837,635 - -0.4 837,635 - -1.5 837,635 - +0.6 837,658 + +0.3 837,658 + -0.0 837,658 + +0.1 837,658 + +0.1 837,658 + -0.9 837,658 + +1.6 837,658 + -0.1 837,658 + -0.0 837,659 - +0.1 837,659 - -0.9 837,660 + -0.1 837,660 + +0.1 837,661 - +0.8 837,661 - -0.0 837,661 - -0.5 837,661 - -0.2 837,661 - +0.1 837,674 + +1.8 837,674 + -0.4 837,675 - +0.3 837,682 + +0.1 837,682 + -0.3 837,682 + -0.2 837,683 - +0.9
Or see this region's nucleotide sequence