Experiment: Glucose (C) and Ammonium chloride (N); with MOPS; with PEG
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS01885 and PFLU_RS01890 are separated by 10 nucleotides PFLU_RS01890 and PFLU_RS01895 are separated by 25 nucleotides PFLU_RS01895 and PFLU_RS01900 are separated by 2 nucleotides PFLU_RS01900 and PFLU_RS01905 are separated by 41 nucleotides
PFLU_RS01885: PFLU_RS01885 - Sec-independent protein translocase subunit TatB, at 426,447 to 426,851
_RS01885
PFLU_RS01890: PFLU_RS01890 - twin-arginine translocase TatA/TatE family subunit, at 426,862 to 427,140
_RS01890
PFLU_RS01895: PFLU_RS01895 - phosphoribosyl-ATP diphosphatase, at 427,166 to 427,498
_RS01895
PFLU_RS01900: PFLU_RS01900 - phosphoribosyl-AMP cyclohydrolase, at 427,501 to 427,893
_RS01900
PFLU_RS01905: PFLU_RS01905 - ubiquinone biosynthesis regulatory protein kinase UbiB, at 427,935 to 429,539
_RS01905
Position (kb) 427
428 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 426.650 kb on + strand, within PFLU_RS01885 at 426.650 kb on + strand, within PFLU_RS01885 at 426.650 kb on + strand, within PFLU_RS01885 at 426.650 kb on + strand, within PFLU_RS01885 at 426.650 kb on + strand, within PFLU_RS01885 at 426.650 kb on + strand, within PFLU_RS01885 at 426.650 kb on + strand, within PFLU_RS01885 at 426.650 kb on + strand, within PFLU_RS01885 at 426.650 kb on + strand, within PFLU_RS01885 at 426.650 kb on + strand, within PFLU_RS01885 at 426.650 kb on + strand, within PFLU_RS01885 at 426.650 kb on + strand, within PFLU_RS01885 at 426.650 kb on + strand, within PFLU_RS01885 at 426.650 kb on + strand, within PFLU_RS01885 at 426.650 kb on + strand, within PFLU_RS01885 at 426.651 kb on - strand, within PFLU_RS01885 at 426.651 kb on - strand, within PFLU_RS01885 at 426.651 kb on - strand, within PFLU_RS01885 at 426.651 kb on - strand, within PFLU_RS01885 at 426.651 kb on - strand, within PFLU_RS01885 at 426.651 kb on - strand, within PFLU_RS01885 at 426.651 kb on - strand, within PFLU_RS01885 at 426.651 kb on - strand, within PFLU_RS01885 at 426.651 kb on - strand, within PFLU_RS01885 at 426.651 kb on - strand, within PFLU_RS01885 at 426.651 kb on - strand, within PFLU_RS01885 at 426.651 kb on - strand, within PFLU_RS01885 at 426.651 kb on - strand, within PFLU_RS01885 at 426.651 kb on - strand, within PFLU_RS01885 at 426.651 kb on - strand, within PFLU_RS01885 at 426.808 kb on - strand, within PFLU_RS01885 at 426.852 kb on + strand at 426.852 kb on + strand at 426.852 kb on + strand at 426.852 kb on + strand at 426.852 kb on + strand at 426.852 kb on + strand at 426.852 kb on + strand at 426.853 kb on - strand at 426.853 kb on - strand at 426.853 kb on - strand at 426.853 kb on - strand at 426.853 kb on - strand at 426.853 kb on - strand at 426.915 kb on + strand, within PFLU_RS01890 at 426.915 kb on + strand, within PFLU_RS01890 at 426.916 kb on - strand, within PFLU_RS01890 at 426.916 kb on - strand, within PFLU_RS01890 at 426.916 kb on - strand, within PFLU_RS01890 at 426.916 kb on - strand, within PFLU_RS01890 at 426.916 kb on - strand, within PFLU_RS01890 at 427.020 kb on + strand, within PFLU_RS01890 at 427.084 kb on + strand, within PFLU_RS01890 at 427.084 kb on + strand, within PFLU_RS01890 at 427.085 kb on - strand, within PFLU_RS01890 at 427.085 kb on - strand, within PFLU_RS01890 at 427.090 kb on + strand, within PFLU_RS01890 at 427.091 kb on - strand, within PFLU_RS01890 at 427.305 kb on + strand, within PFLU_RS01895 at 427.305 kb on + strand, within PFLU_RS01895 at 427.305 kb on + strand, within PFLU_RS01895 at 427.305 kb on + strand, within PFLU_RS01895 at 427.306 kb on - strand, within PFLU_RS01895 at 427.413 kb on + strand, within PFLU_RS01895 at 427.413 kb on + strand, within PFLU_RS01895 at 427.413 kb on + strand, within PFLU_RS01895 at 427.413 kb on + strand, within PFLU_RS01895 at 427.414 kb on - strand, within PFLU_RS01895 at 427.428 kb on + strand, within PFLU_RS01895 at 427.428 kb on + strand, within PFLU_RS01895 at 427.428 kb on + strand, within PFLU_RS01895 at 427.428 kb on + strand, within PFLU_RS01895 at 427.428 kb on + strand, within PFLU_RS01895 at 427.428 kb on + strand, within PFLU_RS01895 at 427.428 kb on + strand, within PFLU_RS01895 at 427.428 kb on + strand, within PFLU_RS01895 at 427.429 kb on - strand, within PFLU_RS01895 at 427.429 kb on - strand, within PFLU_RS01895 at 427.429 kb on - strand, within PFLU_RS01895 at 427.429 kb on - strand, within PFLU_RS01895 at 427.429 kb on - strand, within PFLU_RS01895 at 427.429 kb on - strand, within PFLU_RS01895 at 427.517 kb on + strand at 427.517 kb on + strand at 427.517 kb on + strand at 427.517 kb on + strand at 427.517 kb on + strand at 427.517 kb on + strand at 427.518 kb on - strand at 427.518 kb on - strand at 427.518 kb on - strand at 427.518 kb on - strand at 427.518 kb on - strand at 427.518 kb on - strand at 427.583 kb on + strand, within PFLU_RS01900 at 427.583 kb on + strand, within PFLU_RS01900 at 427.583 kb on + strand, within PFLU_RS01900 at 427.584 kb on - strand, within PFLU_RS01900 at 427.584 kb on - strand, within PFLU_RS01900 at 427.584 kb on - strand, within PFLU_RS01900 at 427.584 kb on - strand, within PFLU_RS01900 at 427.584 kb on - strand, within PFLU_RS01900 at 427.745 kb on + strand, within PFLU_RS01900 at 427.745 kb on + strand, within PFLU_RS01900 at 427.745 kb on + strand, within PFLU_RS01900 at 427.745 kb on + strand, within PFLU_RS01900 at 427.745 kb on + strand, within PFLU_RS01900 at 427.745 kb on + strand, within PFLU_RS01900 at 427.746 kb on - strand, within PFLU_RS01900 at 427.746 kb on - strand, within PFLU_RS01900 at 427.746 kb on - strand, within PFLU_RS01900 at 427.746 kb on - strand, within PFLU_RS01900 at 427.746 kb on - strand, within PFLU_RS01900 at 427.746 kb on - strand, within PFLU_RS01900 at 427.746 kb on - strand, within PFLU_RS01900 at 427.746 kb on - strand, within PFLU_RS01900 at 427.746 kb on - strand, within PFLU_RS01900 at 427.746 kb on - strand, within PFLU_RS01900 at 427.746 kb on - strand, within PFLU_RS01900 at 427.746 kb on - strand, within PFLU_RS01900 at 427.826 kb on + strand, within PFLU_RS01900 at 427.826 kb on + strand, within PFLU_RS01900 at 427.826 kb on + strand, within PFLU_RS01900 at 427.826 kb on + strand, within PFLU_RS01900 at 427.826 kb on + strand, within PFLU_RS01900 at 427.826 kb on + strand, within PFLU_RS01900 at 427.826 kb on + strand, within PFLU_RS01900 at 427.826 kb on + strand, within PFLU_RS01900 at 427.827 kb on - strand, within PFLU_RS01900 at 427.827 kb on - strand, within PFLU_RS01900 at 427.827 kb on - strand, within PFLU_RS01900 at 427.827 kb on - strand, within PFLU_RS01900 at 427.827 kb on - strand, within PFLU_RS01900 at 427.827 kb on - strand, within PFLU_RS01900 at 427.827 kb on - strand, within PFLU_RS01900 at 427.827 kb on - strand, within PFLU_RS01900 at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.921 kb on + strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand at 427.922 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glucose (C) and Ammonium chloride (N); with MOPS; with PEG remove 426,650 + PFLU_RS01885 0.50 +0.5 426,650 + PFLU_RS01885 0.50 -2.2 426,650 + PFLU_RS01885 0.50 -0.6 426,650 + PFLU_RS01885 0.50 -0.8 426,650 + PFLU_RS01885 0.50 -0.2 426,650 + PFLU_RS01885 0.50 +0.5 426,650 + PFLU_RS01885 0.50 -0.9 426,650 + PFLU_RS01885 0.50 +1.3 426,650 + PFLU_RS01885 0.50 -0.5 426,650 + PFLU_RS01885 0.50 -1.2 426,650 + PFLU_RS01885 0.50 -0.2 426,650 + PFLU_RS01885 0.50 -1.2 426,650 + PFLU_RS01885 0.50 +0.3 426,650 + PFLU_RS01885 0.50 -2.4 426,650 + PFLU_RS01885 0.50 -1.2 426,651 - PFLU_RS01885 0.50 -0.8 426,651 - PFLU_RS01885 0.50 -2.6 426,651 - PFLU_RS01885 0.50 -0.9 426,651 - PFLU_RS01885 0.50 -0.2 426,651 - PFLU_RS01885 0.50 -0.5 426,651 - PFLU_RS01885 0.50 +1.2 426,651 - PFLU_RS01885 0.50 +0.4 426,651 - PFLU_RS01885 0.50 +0.8 426,651 - PFLU_RS01885 0.50 -0.2 426,651 - PFLU_RS01885 0.50 -0.3 426,651 - PFLU_RS01885 0.50 +1.3 426,651 - PFLU_RS01885 0.50 +0.2 426,651 - PFLU_RS01885 0.50 -0.8 426,651 - PFLU_RS01885 0.50 -1.8 426,651 - PFLU_RS01885 0.50 +0.7 426,808 - PFLU_RS01885 0.89 -1.4 426,852 + -0.3 426,852 + -1.4 426,852 + -0.5 426,852 + -0.6 426,852 + -0.8 426,852 + -2.2 426,852 + +0.0 426,853 - -0.4 426,853 - +0.4 426,853 - +0.1 426,853 - -1.3 426,853 - -0.3 426,853 - -1.0 426,915 + PFLU_RS01890 0.19 -1.4 426,915 + PFLU_RS01890 0.19 -0.4 426,916 - PFLU_RS01890 0.19 -1.0 426,916 - PFLU_RS01890 0.19 +2.3 426,916 - PFLU_RS01890 0.19 -1.6 426,916 - PFLU_RS01890 0.19 -0.3 426,916 - PFLU_RS01890 0.19 -0.4 427,020 + PFLU_RS01890 0.57 -1.6 427,084 + PFLU_RS01890 0.80 +0.4 427,084 + PFLU_RS01890 0.80 -0.9 427,085 - PFLU_RS01890 0.80 +0.8 427,085 - PFLU_RS01890 0.80 -0.4 427,090 + PFLU_RS01890 0.82 -0.6 427,091 - PFLU_RS01890 0.82 -0.2 427,305 + PFLU_RS01895 0.42 -1.0 427,305 + PFLU_RS01895 0.42 -1.0 427,305 + PFLU_RS01895 0.42 -2.6 427,305 + PFLU_RS01895 0.42 -0.5 427,306 - PFLU_RS01895 0.42 -3.0 427,413 + PFLU_RS01895 0.74 -2.8 427,413 + PFLU_RS01895 0.74 -3.6 427,413 + PFLU_RS01895 0.74 -2.6 427,413 + PFLU_RS01895 0.74 -2.7 427,414 - PFLU_RS01895 0.74 -2.4 427,428 + PFLU_RS01895 0.79 -3.2 427,428 + PFLU_RS01895 0.79 +0.3 427,428 + PFLU_RS01895 0.79 -2.4 427,428 + PFLU_RS01895 0.79 -2.2 427,428 + PFLU_RS01895 0.79 -3.2 427,428 + PFLU_RS01895 0.79 -1.8 427,428 + PFLU_RS01895 0.79 -0.7 427,428 + PFLU_RS01895 0.79 -2.2 427,429 - PFLU_RS01895 0.79 -2.7 427,429 - PFLU_RS01895 0.79 -1.9 427,429 - PFLU_RS01895 0.79 -1.5 427,429 - PFLU_RS01895 0.79 -1.0 427,429 - PFLU_RS01895 0.79 -0.0 427,429 - PFLU_RS01895 0.79 -2.7 427,517 + -2.6 427,517 + -0.6 427,517 + -1.3 427,517 + -1.4 427,517 + -0.0 427,517 + -1.6 427,518 - +1.9 427,518 - -0.5 427,518 - +1.0 427,518 - +1.3 427,518 - -0.5 427,518 - +0.4 427,583 + PFLU_RS01900 0.21 -1.2 427,583 + PFLU_RS01900 0.21 -2.4 427,583 + PFLU_RS01900 0.21 -2.0 427,584 - PFLU_RS01900 0.21 +2.3 427,584 - PFLU_RS01900 0.21 +0.3 427,584 - PFLU_RS01900 0.21 -0.7 427,584 - PFLU_RS01900 0.21 -2.4 427,584 - PFLU_RS01900 0.21 -0.5 427,745 + PFLU_RS01900 0.62 -1.2 427,745 + PFLU_RS01900 0.62 -2.2 427,745 + PFLU_RS01900 0.62 -3.3 427,745 + PFLU_RS01900 0.62 -3.3 427,745 + PFLU_RS01900 0.62 -2.5 427,745 + PFLU_RS01900 0.62 -0.5 427,746 - PFLU_RS01900 0.62 -2.6 427,746 - PFLU_RS01900 0.62 -0.7 427,746 - PFLU_RS01900 0.62 -0.9 427,746 - PFLU_RS01900 0.62 -1.5 427,746 - PFLU_RS01900 0.62 -0.7 427,746 - PFLU_RS01900 0.62 -2.5 427,746 - PFLU_RS01900 0.62 -0.3 427,746 - PFLU_RS01900 0.62 -0.9 427,746 - PFLU_RS01900 0.62 -0.5 427,746 - PFLU_RS01900 0.62 -1.8 427,746 - PFLU_RS01900 0.62 -1.5 427,746 - PFLU_RS01900 0.62 -0.7 427,826 + PFLU_RS01900 0.83 -2.0 427,826 + PFLU_RS01900 0.83 -2.6 427,826 + PFLU_RS01900 0.83 -2.5 427,826 + PFLU_RS01900 0.83 -1.5 427,826 + PFLU_RS01900 0.83 +0.1 427,826 + PFLU_RS01900 0.83 -1.4 427,826 + PFLU_RS01900 0.83 -1.9 427,826 + PFLU_RS01900 0.83 -1.4 427,827 - PFLU_RS01900 0.83 -0.9 427,827 - PFLU_RS01900 0.83 -0.5 427,827 - PFLU_RS01900 0.83 -1.8 427,827 - PFLU_RS01900 0.83 -0.9 427,827 - PFLU_RS01900 0.83 -2.8 427,827 - PFLU_RS01900 0.83 -2.7 427,827 - PFLU_RS01900 0.83 -2.0 427,827 - PFLU_RS01900 0.83 -1.0 427,921 + -2.2 427,921 + +0.9 427,921 + +0.8 427,921 + +0.5 427,921 + +0.0 427,921 + +0.4 427,921 + -1.4 427,921 + -0.6 427,921 + +0.7 427,921 + -0.1 427,921 + +0.5 427,921 + +0.7 427,921 + +0.7 427,921 + -0.4 427,921 + -0.4 427,921 + +0.5 427,921 + -0.5 427,921 + -0.1 427,921 + +0.3 427,921 + +0.4 427,921 + -0.1 427,921 + +0.3 427,921 + -1.0 427,921 + +1.5 427,921 + +0.1 427,921 + -1.4 427,921 + +1.1 427,921 + -0.1 427,921 + -0.7 427,921 + +0.7 427,921 + +0.1 427,921 + -1.0 427,921 + -0.3 427,922 - +0.7 427,922 - -0.1 427,922 - +0.6 427,922 - -1.4 427,922 - +0.2 427,922 - -0.6 427,922 - -0.6 427,922 - +0.4 427,922 - -0.2 427,922 - +0.7 427,922 - -0.2 427,922 - -0.5 427,922 - -0.7 427,922 - +0.0 427,922 - -0.1 427,922 - -1.3 427,922 - +0.4 427,922 - -0.0 427,922 - -0.9 427,922 - -0.0 427,922 - +2.3 427,922 - -1.9 427,922 - +0.7 427,922 - +0.3 427,922 - +0.0 427,922 - -1.2 427,922 - +1.0 427,922 - +1.5 427,922 - -1.4 427,922 - -1.0 427,922 - +0.2 427,922 - -0.2 427,922 - +0.5 427,922 - -1.0 427,922 - +0.4
Or see this region's nucleotide sequence