Experiment: Glucose (C) and Ammonium chloride (N); with MOPS; with PEG
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS13930 and PFLU_RS13935 are separated by 121 nucleotides PFLU_RS13935 and PFLU_RS13940 are separated by 8 nucleotides PFLU_RS13940 and PFLU_RS13945 are separated by 209 nucleotides PFLU_RS13945 and PFLU_RS13950 overlap by 1 nucleotides PFLU_RS13950 and PFLU_RS13955 are separated by 53 nucleotides PFLU_RS13955 and PFLU_RS13960 overlap by 4 nucleotides PFLU_RS13960 and PFLU_RS13965 overlap by 4 nucleotides
PFLU_RS13930: PFLU_RS13930 - hypothetical protein, at 3,132,885 to 3,133,739
_RS13930
PFLU_RS13935: PFLU_RS13935 - Com family DNA-binding transcriptional regulator, at 3,133,861 to 3,134,046
_RS13935
PFLU_RS13940: PFLU_RS13940 - hypothetical protein, at 3,134,055 to 3,134,390
_RS13940
PFLU_RS13945: PFLU_RS13945 - hypothetical protein, at 3,134,600 to 3,134,971
_RS13945
PFLU_RS13950: PFLU_RS13950 - hypothetical protein, at 3,134,971 to 3,135,309
_RS13950
PFLU_RS13955: PFLU_RS13955 - hypothetical protein, at 3,135,363 to 3,135,578
_RS13955
PFLU_RS13960: PFLU_RS13960 - hypothetical protein, at 3,135,575 to 3,135,766
_RS13960
PFLU_RS13965: PFLU_RS13965 - hypothetical protein, at 3,135,763 to 3,136,017
_RS13965
Position (kb) 3134
3135 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 3133.600 kb on + strand, within PFLU_RS13930 at 3133.601 kb on - strand, within PFLU_RS13930 at 3133.601 kb on - strand, within PFLU_RS13930 at 3133.603 kb on + strand, within PFLU_RS13930 at 3133.603 kb on + strand, within PFLU_RS13930 at 3133.604 kb on - strand, within PFLU_RS13930 at 3133.604 kb on - strand, within PFLU_RS13930 at 3133.637 kb on + strand, within PFLU_RS13930 at 3133.637 kb on + strand, within PFLU_RS13930 at 3133.638 kb on - strand, within PFLU_RS13930 at 3133.737 kb on + strand at 3133.737 kb on + strand at 3133.791 kb on + strand at 3133.797 kb on - strand at 3133.797 kb on - strand at 3133.797 kb on - strand at 3134.044 kb on + strand at 3134.044 kb on + strand at 3134.062 kb on + strand at 3134.063 kb on - strand at 3134.063 kb on - strand at 3134.127 kb on - strand, within PFLU_RS13940 at 3134.169 kb on + strand, within PFLU_RS13940 at 3134.180 kb on + strand, within PFLU_RS13940 at 3134.200 kb on + strand, within PFLU_RS13940 at 3134.200 kb on + strand, within PFLU_RS13940 at 3134.200 kb on + strand, within PFLU_RS13940 at 3134.200 kb on + strand, within PFLU_RS13940 at 3134.200 kb on + strand, within PFLU_RS13940 at 3134.200 kb on + strand, within PFLU_RS13940 at 3134.200 kb on + strand, within PFLU_RS13940 at 3134.200 kb on + strand, within PFLU_RS13940 at 3134.200 kb on + strand, within PFLU_RS13940 at 3134.201 kb on - strand, within PFLU_RS13940 at 3134.201 kb on - strand, within PFLU_RS13940 at 3134.201 kb on - strand, within PFLU_RS13940 at 3134.201 kb on - strand, within PFLU_RS13940 at 3134.201 kb on - strand, within PFLU_RS13940 at 3134.201 kb on - strand, within PFLU_RS13940 at 3134.235 kb on - strand, within PFLU_RS13940 at 3134.245 kb on + strand, within PFLU_RS13940 at 3134.259 kb on - strand, within PFLU_RS13940 at 3134.259 kb on - strand, within PFLU_RS13940 at 3134.309 kb on - strand, within PFLU_RS13940 at 3134.309 kb on - strand, within PFLU_RS13940 at 3134.309 kb on - strand, within PFLU_RS13940 at 3134.310 kb on + strand, within PFLU_RS13940 at 3134.310 kb on + strand, within PFLU_RS13940 at 3134.310 kb on + strand, within PFLU_RS13940 at 3134.310 kb on + strand, within PFLU_RS13940 at 3134.310 kb on + strand, within PFLU_RS13940 at 3134.310 kb on + strand, within PFLU_RS13940 at 3134.310 kb on + strand, within PFLU_RS13940 at 3134.310 kb on + strand, within PFLU_RS13940 at 3134.310 kb on + strand, within PFLU_RS13940 at 3134.310 kb on + strand, within PFLU_RS13940 at 3134.310 kb on + strand, within PFLU_RS13940 at 3134.310 kb on + strand, within PFLU_RS13940 at 3134.310 kb on + strand, within PFLU_RS13940 at 3134.310 kb on + strand, within PFLU_RS13940 at 3134.310 kb on + strand, within PFLU_RS13940 at 3134.310 kb on + strand, within PFLU_RS13940 at 3134.310 kb on + strand, within PFLU_RS13940 at 3134.310 kb on + strand, within PFLU_RS13940 at 3134.311 kb on - strand, within PFLU_RS13940 at 3134.311 kb on - strand, within PFLU_RS13940 at 3134.311 kb on - strand, within PFLU_RS13940 at 3134.311 kb on - strand, within PFLU_RS13940 at 3134.311 kb on - strand, within PFLU_RS13940 at 3134.311 kb on - strand, within PFLU_RS13940 at 3134.311 kb on - strand, within PFLU_RS13940 at 3134.311 kb on - strand, within PFLU_RS13940 at 3134.311 kb on - strand, within PFLU_RS13940 at 3134.311 kb on - strand, within PFLU_RS13940 at 3134.311 kb on - strand, within PFLU_RS13940 at 3134.311 kb on - strand, within PFLU_RS13940 at 3134.312 kb on + strand, within PFLU_RS13940 at 3134.312 kb on + strand, within PFLU_RS13940 at 3134.312 kb on + strand, within PFLU_RS13940 at 3134.312 kb on + strand, within PFLU_RS13940 at 3134.312 kb on + strand, within PFLU_RS13940 at 3134.312 kb on + strand, within PFLU_RS13940 at 3134.312 kb on + strand, within PFLU_RS13940 at 3134.312 kb on + strand, within PFLU_RS13940 at 3134.312 kb on + strand, within PFLU_RS13940 at 3134.312 kb on + strand, within PFLU_RS13940 at 3134.312 kb on + strand, within PFLU_RS13940 at 3134.312 kb on + strand, within PFLU_RS13940 at 3134.312 kb on + strand, within PFLU_RS13940 at 3134.312 kb on + strand, within PFLU_RS13940 at 3134.312 kb on + strand, within PFLU_RS13940 at 3134.312 kb on + strand, within PFLU_RS13940 at 3134.313 kb on - strand, within PFLU_RS13940 at 3134.313 kb on - strand, within PFLU_RS13940 at 3134.313 kb on - strand, within PFLU_RS13940 at 3134.313 kb on - strand, within PFLU_RS13940 at 3134.313 kb on - strand, within PFLU_RS13940 at 3134.313 kb on - strand, within PFLU_RS13940 at 3134.313 kb on - strand, within PFLU_RS13940 at 3134.313 kb on - strand, within PFLU_RS13940 at 3134.313 kb on - strand, within PFLU_RS13940 at 3134.313 kb on - strand, within PFLU_RS13940 at 3134.313 kb on - strand, within PFLU_RS13940 at 3134.313 kb on - strand, within PFLU_RS13940 at 3134.313 kb on - strand, within PFLU_RS13940 at 3134.313 kb on - strand, within PFLU_RS13940 at 3134.313 kb on - strand, within PFLU_RS13940 at 3134.332 kb on - strand, within PFLU_RS13940 at 3134.346 kb on + strand, within PFLU_RS13940 at 3134.346 kb on + strand, within PFLU_RS13940 at 3134.346 kb on + strand, within PFLU_RS13940 at 3134.346 kb on + strand, within PFLU_RS13940 at 3134.347 kb on - strand, within PFLU_RS13940 at 3134.347 kb on - strand, within PFLU_RS13940 at 3134.347 kb on - strand, within PFLU_RS13940 at 3134.347 kb on - strand, within PFLU_RS13940 at 3134.348 kb on + strand, within PFLU_RS13940 at 3134.349 kb on - strand, within PFLU_RS13940 at 3134.349 kb on - strand, within PFLU_RS13940 at 3134.361 kb on + strand at 3134.417 kb on + strand at 3134.417 kb on + strand at 3134.417 kb on + strand at 3134.417 kb on + strand at 3134.418 kb on - strand at 3134.418 kb on - strand at 3134.418 kb on - strand at 3134.418 kb on - strand at 3134.418 kb on - strand at 3134.418 kb on - strand at 3134.418 kb on - strand at 3134.418 kb on - strand at 3134.451 kb on + strand at 3134.464 kb on + strand at 3134.464 kb on + strand at 3134.464 kb on + strand at 3134.465 kb on - strand at 3134.465 kb on - strand at 3134.465 kb on - strand at 3134.465 kb on - strand at 3134.554 kb on + strand at 3134.554 kb on + strand at 3134.554 kb on + strand at 3134.554 kb on + strand at 3134.555 kb on - strand at 3134.555 kb on - strand at 3134.555 kb on - strand at 3134.555 kb on - strand at 3134.555 kb on - strand at 3134.555 kb on - strand at 3134.555 kb on - strand at 3134.560 kb on + strand at 3134.560 kb on + strand at 3134.560 kb on + strand at 3134.560 kb on + strand at 3134.561 kb on - strand at 3134.569 kb on + strand at 3134.570 kb on - strand at 3134.570 kb on - strand at 3134.588 kb on + strand at 3134.588 kb on + strand at 3134.608 kb on + strand at 3134.608 kb on + strand at 3134.902 kb on + strand, within PFLU_RS13945 at 3134.903 kb on - strand, within PFLU_RS13945 at 3134.903 kb on - strand, within PFLU_RS13945 at 3134.903 kb on - strand, within PFLU_RS13945 at 3135.139 kb on + strand, within PFLU_RS13950 at 3135.139 kb on + strand, within PFLU_RS13950 at 3135.140 kb on - strand, within PFLU_RS13950 at 3135.140 kb on - strand, within PFLU_RS13950 at 3135.140 kb on - strand, within PFLU_RS13950 at 3135.450 kb on + strand, within PFLU_RS13955 at 3135.450 kb on + strand, within PFLU_RS13955 at 3135.450 kb on + strand, within PFLU_RS13955 at 3135.450 kb on + strand, within PFLU_RS13955 at 3135.450 kb on + strand, within PFLU_RS13955 at 3135.450 kb on + strand, within PFLU_RS13955 at 3135.450 kb on + strand, within PFLU_RS13955 at 3135.450 kb on + strand, within PFLU_RS13955 at 3135.450 kb on + strand, within PFLU_RS13955 at 3135.450 kb on + strand, within PFLU_RS13955 at 3135.450 kb on + strand, within PFLU_RS13955 at 3135.450 kb on + strand, within PFLU_RS13955 at 3135.450 kb on + strand, within PFLU_RS13955 at 3135.451 kb on - strand, within PFLU_RS13955 at 3135.451 kb on - strand, within PFLU_RS13955 at 3135.451 kb on - strand, within PFLU_RS13955 at 3135.451 kb on - strand, within PFLU_RS13955 at 3135.451 kb on - strand, within PFLU_RS13955 at 3135.451 kb on - strand, within PFLU_RS13955 at 3135.451 kb on - strand, within PFLU_RS13955 at 3135.451 kb on - strand, within PFLU_RS13955 at 3135.507 kb on + strand, within PFLU_RS13955 at 3135.507 kb on + strand, within PFLU_RS13955 at 3135.507 kb on + strand, within PFLU_RS13955 at 3135.507 kb on + strand, within PFLU_RS13955 at 3135.508 kb on - strand, within PFLU_RS13955 at 3135.508 kb on - strand, within PFLU_RS13955 at 3135.636 kb on + strand, within PFLU_RS13960 at 3135.636 kb on + strand, within PFLU_RS13960 at 3135.930 kb on + strand, within PFLU_RS13965 at 3135.931 kb on - strand, within PFLU_RS13965 at 3135.931 kb on - strand, within PFLU_RS13965 at 3135.960 kb on + strand, within PFLU_RS13965 at 3135.960 kb on + strand, within PFLU_RS13965 at 3135.960 kb on + strand, within PFLU_RS13965 at 3135.961 kb on - strand, within PFLU_RS13965 at 3135.961 kb on - strand, within PFLU_RS13965 at 3135.961 kb on - strand, within PFLU_RS13965
Per-strain Table
Position Strand Gene LocusTag Fraction Glucose (C) and Ammonium chloride (N); with MOPS; with PEG remove 3,133,600 + PFLU_RS13930 0.84 +0.1 3,133,601 - PFLU_RS13930 0.84 -1.4 3,133,601 - PFLU_RS13930 0.84 +2.1 3,133,603 + PFLU_RS13930 0.84 -1.3 3,133,603 + PFLU_RS13930 0.84 +0.2 3,133,604 - PFLU_RS13930 0.84 +0.6 3,133,604 - PFLU_RS13930 0.84 -1.4 3,133,637 + PFLU_RS13930 0.88 +0.8 3,133,637 + PFLU_RS13930 0.88 -0.7 3,133,638 - PFLU_RS13930 0.88 +0.3 3,133,737 + -0.7 3,133,737 + +1.4 3,133,791 + -0.5 3,133,797 - +1.6 3,133,797 - +1.4 3,133,797 - +2.2 3,134,044 + -0.2 3,134,044 + +0.5 3,134,062 + -1.4 3,134,063 - +0.1 3,134,063 - +0.5 3,134,127 - PFLU_RS13940 0.21 +0.5 3,134,169 + PFLU_RS13940 0.34 -0.3 3,134,180 + PFLU_RS13940 0.37 -0.5 3,134,200 + PFLU_RS13940 0.43 -1.4 3,134,200 + PFLU_RS13940 0.43 -1.5 3,134,200 + PFLU_RS13940 0.43 -0.6 3,134,200 + PFLU_RS13940 0.43 +0.3 3,134,200 + PFLU_RS13940 0.43 +0.4 3,134,200 + PFLU_RS13940 0.43 +0.5 3,134,200 + PFLU_RS13940 0.43 +0.4 3,134,200 + PFLU_RS13940 0.43 -0.1 3,134,200 + PFLU_RS13940 0.43 -0.3 3,134,201 - PFLU_RS13940 0.43 +0.6 3,134,201 - PFLU_RS13940 0.43 -1.7 3,134,201 - PFLU_RS13940 0.43 +1.5 3,134,201 - PFLU_RS13940 0.43 +0.1 3,134,201 - PFLU_RS13940 0.43 -0.3 3,134,201 - PFLU_RS13940 0.43 -0.7 3,134,235 - PFLU_RS13940 0.54 +0.6 3,134,245 + PFLU_RS13940 0.57 -0.8 3,134,259 - PFLU_RS13940 0.61 -1.3 3,134,259 - PFLU_RS13940 0.61 -0.5 3,134,309 - PFLU_RS13940 0.76 +0.1 3,134,309 - PFLU_RS13940 0.76 +3.6 3,134,309 - PFLU_RS13940 0.76 -0.3 3,134,310 + PFLU_RS13940 0.76 +0.1 3,134,310 + PFLU_RS13940 0.76 -0.1 3,134,310 + PFLU_RS13940 0.76 +1.1 3,134,310 + PFLU_RS13940 0.76 -2.5 3,134,310 + PFLU_RS13940 0.76 +0.3 3,134,310 + PFLU_RS13940 0.76 -2.3 3,134,310 + PFLU_RS13940 0.76 -0.2 3,134,310 + PFLU_RS13940 0.76 -0.8 3,134,310 + PFLU_RS13940 0.76 +1.5 3,134,310 + PFLU_RS13940 0.76 +0.4 3,134,310 + PFLU_RS13940 0.76 -0.5 3,134,310 + PFLU_RS13940 0.76 +0.4 3,134,310 + PFLU_RS13940 0.76 -0.8 3,134,310 + PFLU_RS13940 0.76 -2.7 3,134,310 + PFLU_RS13940 0.76 -0.5 3,134,310 + PFLU_RS13940 0.76 +0.6 3,134,310 + PFLU_RS13940 0.76 -1.7 3,134,310 + PFLU_RS13940 0.76 +0.5 3,134,311 - PFLU_RS13940 0.76 +1.6 3,134,311 - PFLU_RS13940 0.76 +0.1 3,134,311 - PFLU_RS13940 0.76 +1.6 3,134,311 - PFLU_RS13940 0.76 +0.6 3,134,311 - PFLU_RS13940 0.76 -0.1 3,134,311 - PFLU_RS13940 0.76 -0.4 3,134,311 - PFLU_RS13940 0.76 -0.3 3,134,311 - PFLU_RS13940 0.76 +1.1 3,134,311 - PFLU_RS13940 0.76 -0.2 3,134,311 - PFLU_RS13940 0.76 +0.5 3,134,311 - PFLU_RS13940 0.76 -0.8 3,134,311 - PFLU_RS13940 0.76 +0.5 3,134,312 + PFLU_RS13940 0.76 -0.7 3,134,312 + PFLU_RS13940 0.76 -0.0 3,134,312 + PFLU_RS13940 0.76 +0.9 3,134,312 + PFLU_RS13940 0.76 +0.6 3,134,312 + PFLU_RS13940 0.76 -1.6 3,134,312 + PFLU_RS13940 0.76 -0.6 3,134,312 + PFLU_RS13940 0.76 +1.1 3,134,312 + PFLU_RS13940 0.76 +1.1 3,134,312 + PFLU_RS13940 0.76 +1.2 3,134,312 + PFLU_RS13940 0.76 -0.1 3,134,312 + PFLU_RS13940 0.76 +0.5 3,134,312 + PFLU_RS13940 0.76 -0.9 3,134,312 + PFLU_RS13940 0.76 -0.4 3,134,312 + PFLU_RS13940 0.76 -0.9 3,134,312 + PFLU_RS13940 0.76 -1.7 3,134,312 + PFLU_RS13940 0.76 -1.1 3,134,313 - PFLU_RS13940 0.77 -0.6 3,134,313 - PFLU_RS13940 0.77 +1.6 3,134,313 - PFLU_RS13940 0.77 +0.5 3,134,313 - PFLU_RS13940 0.77 -0.1 3,134,313 - PFLU_RS13940 0.77 +2.4 3,134,313 - PFLU_RS13940 0.77 -1.9 3,134,313 - PFLU_RS13940 0.77 -0.4 3,134,313 - PFLU_RS13940 0.77 +0.6 3,134,313 - PFLU_RS13940 0.77 +0.9 3,134,313 - PFLU_RS13940 0.77 +0.5 3,134,313 - PFLU_RS13940 0.77 +1.1 3,134,313 - PFLU_RS13940 0.77 +0.1 3,134,313 - PFLU_RS13940 0.77 +0.6 3,134,313 - PFLU_RS13940 0.77 -1.1 3,134,313 - PFLU_RS13940 0.77 +0.9 3,134,332 - PFLU_RS13940 0.82 +1.2 3,134,346 + PFLU_RS13940 0.87 +0.4 3,134,346 + PFLU_RS13940 0.87 +0.5 3,134,346 + PFLU_RS13940 0.87 -1.4 3,134,346 + PFLU_RS13940 0.87 +0.4 3,134,347 - PFLU_RS13940 0.87 +0.2 3,134,347 - PFLU_RS13940 0.87 +0.6 3,134,347 - PFLU_RS13940 0.87 -0.1 3,134,347 - PFLU_RS13940 0.87 -0.1 3,134,348 + PFLU_RS13940 0.87 +1.6 3,134,349 - PFLU_RS13940 0.88 +1.1 3,134,349 - PFLU_RS13940 0.88 -0.8 3,134,361 + -0.3 3,134,417 + +2.2 3,134,417 + +0.3 3,134,417 + +0.4 3,134,417 + -0.8 3,134,418 - -2.3 3,134,418 - +0.7 3,134,418 - +1.1 3,134,418 - -1.6 3,134,418 - +0.1 3,134,418 - -0.5 3,134,418 - +0.8 3,134,418 - +0.7 3,134,451 + -1.7 3,134,464 + -0.2 3,134,464 + -1.3 3,134,464 + -0.8 3,134,465 - +0.7 3,134,465 - +0.1 3,134,465 - -0.2 3,134,465 - -0.1 3,134,554 + -1.3 3,134,554 + +0.1 3,134,554 + -0.9 3,134,554 + +0.5 3,134,555 - -0.0 3,134,555 - +1.1 3,134,555 - +0.1 3,134,555 - +0.3 3,134,555 - +0.2 3,134,555 - -0.5 3,134,555 - +0.5 3,134,560 + -1.3 3,134,560 + -0.1 3,134,560 + +0.1 3,134,560 + +0.3 3,134,561 - -0.1 3,134,569 + +0.3 3,134,570 - +0.1 3,134,570 - +1.1 3,134,588 + +0.4 3,134,588 + +0.4 3,134,608 + +2.1 3,134,608 + -1.7 3,134,902 + PFLU_RS13945 0.81 -0.7 3,134,903 - PFLU_RS13945 0.81 -1.3 3,134,903 - PFLU_RS13945 0.81 -0.2 3,134,903 - PFLU_RS13945 0.81 -2.0 3,135,139 + PFLU_RS13950 0.50 -0.6 3,135,139 + PFLU_RS13950 0.50 -0.2 3,135,140 - PFLU_RS13950 0.50 -0.3 3,135,140 - PFLU_RS13950 0.50 +0.1 3,135,140 - PFLU_RS13950 0.50 +0.3 3,135,450 + PFLU_RS13955 0.40 +1.6 3,135,450 + PFLU_RS13955 0.40 -0.3 3,135,450 + PFLU_RS13955 0.40 +1.1 3,135,450 + PFLU_RS13955 0.40 +0.1 3,135,450 + PFLU_RS13955 0.40 +2.6 3,135,450 + PFLU_RS13955 0.40 -0.4 3,135,450 + PFLU_RS13955 0.40 +0.6 3,135,450 + PFLU_RS13955 0.40 +1.1 3,135,450 + PFLU_RS13955 0.40 -0.1 3,135,450 + PFLU_RS13955 0.40 +0.4 3,135,450 + PFLU_RS13955 0.40 -0.7 3,135,450 + PFLU_RS13955 0.40 +0.8 3,135,450 + PFLU_RS13955 0.40 +0.1 3,135,451 - PFLU_RS13955 0.41 +1.1 3,135,451 - PFLU_RS13955 0.41 -0.2 3,135,451 - PFLU_RS13955 0.41 -2.9 3,135,451 - PFLU_RS13955 0.41 -0.9 3,135,451 - PFLU_RS13955 0.41 -1.5 3,135,451 - PFLU_RS13955 0.41 -0.4 3,135,451 - PFLU_RS13955 0.41 +0.6 3,135,451 - PFLU_RS13955 0.41 +0.3 3,135,507 + PFLU_RS13955 0.67 -2.0 3,135,507 + PFLU_RS13955 0.67 +0.5 3,135,507 + PFLU_RS13955 0.67 -0.2 3,135,507 + PFLU_RS13955 0.67 -0.3 3,135,508 - PFLU_RS13955 0.67 -1.9 3,135,508 - PFLU_RS13955 0.67 +1.3 3,135,636 + PFLU_RS13960 0.32 +0.7 3,135,636 + PFLU_RS13960 0.32 -0.9 3,135,930 + PFLU_RS13965 0.65 -0.5 3,135,931 - PFLU_RS13965 0.66 -0.5 3,135,931 - PFLU_RS13965 0.66 -0.9 3,135,960 + PFLU_RS13965 0.77 -0.5 3,135,960 + PFLU_RS13965 0.77 -0.5 3,135,960 + PFLU_RS13965 0.77 +1.7 3,135,961 - PFLU_RS13965 0.78 -0.1 3,135,961 - PFLU_RS13965 0.78 +0.6 3,135,961 - PFLU_RS13965 0.78 -2.2
Or see this region's nucleotide sequence