Experiment: Post-colonization (Day 14) - Cage3;Mouse3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0248 and BT0249 are separated by 69 nucleotides BT0249 and BT0250 are separated by 25 nucleotides BT0250 and BT0251 overlap by 4 nucleotides BT0251 and BT0252 are separated by 244 nucleotides
BT0248: BT0248 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 273,972 to 274,481
BT0248
BT0249: BT0249 - 5-methyltetrahydrofolate--homocysteine methyltransferase or methionine synthase (NCBI ptt file), at 274,551 to 275,453
BT0249
BT0250: BT0250 - dihydroorotase (DHOase) (NCBI ptt file), at 275,479 to 276,816
BT0250
BT0251: BT0251 - dolichol-phosphate mannosyltransferase (NCBI ptt file), at 276,813 to 277,556
BT0251
BT0252: BT0252 - transcription-repair coupling factor (NCBI ptt file), at 277,801 to 281,184
BT0252
Position (kb) 275
276
277 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 274.542 kb on - strand at 274.616 kb on - strand at 274.831 kb on + strand, within BT0249 at 274.899 kb on + strand, within BT0249 at 274.899 kb on + strand, within BT0249 at 274.903 kb on + strand, within BT0249 at 275.011 kb on - strand, within BT0249 at 275.011 kb on - strand, within BT0249 at 275.172 kb on - strand, within BT0249 at 275.402 kb on - strand at 275.434 kb on - strand at 275.438 kb on + strand at 275.438 kb on + strand at 275.439 kb on - strand at 275.439 kb on - strand at 275.439 kb on - strand at 275.453 kb on + strand at 275.454 kb on - strand at 275.459 kb on + strand at 275.460 kb on - strand at 277.603 kb on - strand at 277.618 kb on - strand at 277.801 kb on - strand at 277.801 kb on - strand at 277.801 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Post-colonization (Day 14) - Cage3;Mouse3 remove 274,542 - +0.5 274,616 - -1.9 274,831 + BT0249 0.31 -1.6 274,899 + BT0249 0.39 -2.9 274,899 + BT0249 0.39 -1.6 274,903 + BT0249 0.39 -2.1 275,011 - BT0249 0.51 -1.1 275,011 - BT0249 0.51 -2.1 275,172 - BT0249 0.69 -1.6 275,402 - -1.6 275,434 - -3.2 275,438 + -3.6 275,438 + -4.5 275,439 - -1.8 275,439 - -0.8 275,439 - -0.4 275,453 + -1.4 275,454 - -2.2 275,459 + -1.9 275,460 - -0.9 277,603 - -0.4 277,618 - -3.2 277,801 - -2.6 277,801 - +0.9 277,801 - -0.9
Or see this region's nucleotide sequence