Experiment: L-tyrosine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0276 and Psest_0277 are separated by 65 nucleotides Psest_0277 and Psest_0278 overlap by 4 nucleotides Psest_0278 and Psest_0279 are separated by 26 nucleotides
Psest_0276: Psest_0276 - putative oxygen-independent coproporphyrinogen III oxidase, at 291,796 to 292,929
_0276
Psest_0277: Psest_0277 - non-canonical purine NTP pyrophosphatase, rdgB/HAM1 family, at 292,995 to 293,588
_0277
Psest_0278: Psest_0278 - hypothetical protein, at 293,585 to 294,007
_0278
Psest_0279: Psest_0279 - methionine biosynthesis protein MetW, at 294,034 to 294,633
_0279
Position (kb) 292
293
294 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 292.067 kb on + strand, within Psest_0276 at 292.106 kb on + strand, within Psest_0276 at 292.107 kb on - strand, within Psest_0276 at 292.107 kb on - strand, within Psest_0276 at 292.176 kb on - strand, within Psest_0276 at 292.176 kb on - strand, within Psest_0276 at 292.229 kb on + strand, within Psest_0276 at 292.230 kb on - strand, within Psest_0276 at 292.598 kb on + strand, within Psest_0276 at 292.820 kb on + strand at 292.821 kb on - strand at 292.821 kb on - strand at 292.907 kb on + strand at 292.907 kb on + strand at 292.908 kb on - strand at 292.956 kb on + strand at 292.957 kb on - strand at 292.999 kb on - strand at 293.332 kb on + strand, within Psest_0277 at 293.332 kb on + strand, within Psest_0277 at 293.333 kb on - strand, within Psest_0277 at 293.649 kb on - strand, within Psest_0278 at 293.703 kb on + strand, within Psest_0278 at 293.703 kb on + strand, within Psest_0278 at 293.704 kb on - strand, within Psest_0278 at 293.704 kb on - strand, within Psest_0278 at 293.704 kb on - strand, within Psest_0278 at 293.706 kb on + strand, within Psest_0278 at 293.706 kb on + strand, within Psest_0278 at 293.706 kb on + strand, within Psest_0278 at 293.706 kb on + strand, within Psest_0278 at 293.706 kb on + strand, within Psest_0278 at 293.707 kb on - strand, within Psest_0278 at 293.707 kb on - strand, within Psest_0278 at 293.707 kb on - strand, within Psest_0278 at 293.707 kb on - strand, within Psest_0278 at 293.707 kb on - strand, within Psest_0278 at 293.913 kb on + strand, within Psest_0278 at 293.913 kb on + strand, within Psest_0278 at 293.913 kb on + strand, within Psest_0278 at 293.913 kb on + strand, within Psest_0278 at 293.914 kb on - strand, within Psest_0278 at 293.914 kb on - strand, within Psest_0278 at 293.914 kb on - strand, within Psest_0278 at 293.914 kb on - strand, within Psest_0278 at 293.914 kb on - strand, within Psest_0278 at 293.914 kb on - strand, within Psest_0278 at 293.914 kb on - strand, within Psest_0278 at 293.914 kb on - strand, within Psest_0278 at 293.925 kb on + strand, within Psest_0278 at 293.926 kb on - strand, within Psest_0278 at 293.926 kb on - strand, within Psest_0278 at 293.926 kb on - strand, within Psest_0278 at 294.007 kb on + strand at 294.007 kb on + strand at 294.007 kb on + strand at 294.008 kb on - strand at 294.008 kb on - strand at 294.013 kb on + strand at 294.059 kb on + strand at 294.060 kb on - strand at 294.218 kb on + strand, within Psest_0279 at 294.218 kb on + strand, within Psest_0279 at 294.219 kb on - strand, within Psest_0279 at 294.219 kb on - strand, within Psest_0279 at 294.219 kb on - strand, within Psest_0279 at 294.219 kb on - strand, within Psest_0279 at 294.219 kb on - strand, within Psest_0279 at 294.219 kb on - strand, within Psest_0279 at 294.219 kb on - strand, within Psest_0279 at 294.227 kb on + strand, within Psest_0279 at 294.227 kb on + strand, within Psest_0279 at 294.228 kb on - strand, within Psest_0279 at 294.228 kb on - strand, within Psest_0279
Per-strain Table
Position Strand Gene LocusTag Fraction L-tyrosine remove 292,067 + Psest_0276 0.24 -0.2 292,106 + Psest_0276 0.27 -0.4 292,107 - Psest_0276 0.27 -0.6 292,107 - Psest_0276 0.27 -1.3 292,176 - Psest_0276 0.34 -0.8 292,176 - Psest_0276 0.34 -0.6 292,229 + Psest_0276 0.38 -0.8 292,230 - Psest_0276 0.38 -1.4 292,598 + Psest_0276 0.71 -0.6 292,820 + +0.0 292,821 - -0.8 292,821 - -1.9 292,907 + -1.0 292,907 + -0.8 292,908 - -1.4 292,956 + +3.5 292,957 - +0.1 292,999 - +0.7 293,332 + Psest_0277 0.57 -1.4 293,332 + Psest_0277 0.57 +2.9 293,333 - Psest_0277 0.57 +0.4 293,649 - Psest_0278 0.15 -1.1 293,703 + Psest_0278 0.28 -1.5 293,703 + Psest_0278 0.28 -1.5 293,704 - Psest_0278 0.28 +0.9 293,704 - Psest_0278 0.28 +2.9 293,704 - Psest_0278 0.28 +1.2 293,706 + Psest_0278 0.29 +2.0 293,706 + Psest_0278 0.29 +0.2 293,706 + Psest_0278 0.29 +2.4 293,706 + Psest_0278 0.29 +1.4 293,706 + Psest_0278 0.29 +1.4 293,707 - Psest_0278 0.29 -0.7 293,707 - Psest_0278 0.29 +0.5 293,707 - Psest_0278 0.29 -1.3 293,707 - Psest_0278 0.29 -1.6 293,707 - Psest_0278 0.29 +0.4 293,913 + Psest_0278 0.78 -0.2 293,913 + Psest_0278 0.78 +0.2 293,913 + Psest_0278 0.78 -0.2 293,913 + Psest_0278 0.78 -0.5 293,914 - Psest_0278 0.78 +1.2 293,914 - Psest_0278 0.78 +0.3 293,914 - Psest_0278 0.78 +0.1 293,914 - Psest_0278 0.78 -1.3 293,914 - Psest_0278 0.78 -0.8 293,914 - Psest_0278 0.78 -1.0 293,914 - Psest_0278 0.78 -0.1 293,914 - Psest_0278 0.78 -0.4 293,925 + Psest_0278 0.80 +0.4 293,926 - Psest_0278 0.81 +0.5 293,926 - Psest_0278 0.81 -0.4 293,926 - Psest_0278 0.81 -0.9 294,007 + +1.3 294,007 + -0.2 294,007 + +1.2 294,008 - -0.2 294,008 - +0.1 294,013 + -1.6 294,059 + -2.7 294,060 - +0.4 294,218 + Psest_0279 0.31 -1.4 294,218 + Psest_0279 0.31 -2.5 294,219 - Psest_0279 0.31 -4.5 294,219 - Psest_0279 0.31 -3.4 294,219 - Psest_0279 0.31 +1.4 294,219 - Psest_0279 0.31 -4.3 294,219 - Psest_0279 0.31 -4.3 294,219 - Psest_0279 0.31 -3.1 294,219 - Psest_0279 0.31 -3.4 294,227 + Psest_0279 0.32 -4.0 294,227 + Psest_0279 0.32 -1.9 294,228 - Psest_0279 0.32 -3.5 294,228 - Psest_0279 0.32 -2.7
Or see this region's nucleotide sequence