Experiment: L-tyrosine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1106 and Psest_1107 are separated by 30 nucleotides Psest_1107 and Psest_1108 are separated by 58 nucleotides Psest_1108 and Psest_1109 are separated by 19 nucleotides Psest_1109 and Psest_1110 are separated by 0 nucleotides Psest_1110 and Psest_1111 are separated by 3 nucleotides
Psest_1106: Psest_1106 - ribose-phosphate pyrophosphokinase, at 1,201,892 to 1,202,881
_1106
Psest_1107: Psest_1107 - tRNA_Lys_TTT, at 1,202,912 to 1,202,987
_1107
Psest_1108: Psest_1108 - tRNA_Gln_TTG, at 1,203,046 to 1,203,120
_1108
Psest_1109: Psest_1109 - 4-diphosphocytidyl-2C-methyl-D-erythritol kinase, at 1,203,140 to 1,204,000
_1109
Psest_1110: Psest_1110 - outer membrane lipoprotein LolB, at 1,204,001 to 1,204,621
_1110
Psest_1111: Psest_1111 - Flp pilus assembly protein TadD, contains TPR repeats, at 1,204,625 to 1,206,352
_1111
Position (kb)
1203
1204
1205 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1203.003 kb on - strand at 1203.003 kb on - strand at 1203.003 kb on - strand at 1203.003 kb on - strand at 1203.003 kb on - strand at 1203.028 kb on - strand at 1203.028 kb on - strand at 1204.689 kb on - strand at 1204.689 kb on - strand at 1204.770 kb on - strand at 1204.770 kb on - strand at 1204.808 kb on + strand, within Psest_1111 at 1204.896 kb on + strand, within Psest_1111 at 1204.896 kb on + strand, within Psest_1111 at 1204.896 kb on + strand, within Psest_1111
Per-strain Table
Position Strand Gene LocusTag Fraction L-tyrosine remove 1,203,003 - -2.0 1,203,003 - -1.0 1,203,003 - -0.0 1,203,003 - +0.8 1,203,003 - -2.0 1,203,028 - -1.9 1,203,028 - +1.3 1,204,689 - -0.4 1,204,689 - +0.4 1,204,770 - -1.5 1,204,770 - -1.7 1,204,808 + Psest_1111 0.11 +0.3 1,204,896 + Psest_1111 0.16 +3.1 1,204,896 + Psest_1111 0.16 +1.1 1,204,896 + Psest_1111 0.16 +3.5
Or see this region's nucleotide sequence