Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0496 and Psest_0497 are separated by 9 nucleotides Psest_0497 and Psest_0498 are separated by 9 nucleotides
Psest_0496: Psest_0496 - flagellar motor stator protein MotA, at 551,076 to 551,927
_0496
Psest_0497: Psest_0497 - Flagellar motor protein, at 551,937 to 552,917
_0497
Psest_0498: Psest_0498 - ribosome small subunit-dependent GTPase A, at 552,927 to 553,958
_0498
Position (kb) 551
552
553 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 551.069 kb on + strand at 551.069 kb on + strand at 551.070 kb on - strand at 551.070 kb on - strand at 551.075 kb on + strand at 551.075 kb on + strand at 551.075 kb on + strand at 551.075 kb on + strand at 551.076 kb on - strand at 551.293 kb on - strand, within Psest_0496 at 551.293 kb on - strand, within Psest_0496 at 551.293 kb on - strand, within Psest_0496 at 551.295 kb on + strand, within Psest_0496 at 551.295 kb on + strand, within Psest_0496 at 551.295 kb on + strand, within Psest_0496 at 551.320 kb on - strand, within Psest_0496 at 551.409 kb on + strand, within Psest_0496 at 551.409 kb on + strand, within Psest_0496 at 551.410 kb on - strand, within Psest_0496 at 551.410 kb on - strand, within Psest_0496 at 551.410 kb on - strand, within Psest_0496 at 551.457 kb on + strand, within Psest_0496 at 551.457 kb on + strand, within Psest_0496 at 551.458 kb on - strand, within Psest_0496 at 551.458 kb on - strand, within Psest_0496 at 551.458 kb on - strand, within Psest_0496 at 551.673 kb on + strand, within Psest_0496 at 551.725 kb on - strand, within Psest_0496 at 551.725 kb on - strand, within Psest_0496 at 551.725 kb on - strand, within Psest_0496 at 551.925 kb on + strand at 551.926 kb on - strand at 552.101 kb on + strand, within Psest_0497 at 552.101 kb on + strand, within Psest_0497 at 552.102 kb on - strand, within Psest_0497 at 552.112 kb on - strand, within Psest_0497 at 552.459 kb on + strand, within Psest_0497 at 552.459 kb on + strand, within Psest_0497 at 552.460 kb on - strand, within Psest_0497 at 552.460 kb on - strand, within Psest_0497 at 552.460 kb on - strand, within Psest_0497 at 552.460 kb on - strand, within Psest_0497 at 552.491 kb on + strand, within Psest_0497 at 552.492 kb on - strand, within Psest_0497 at 552.722 kb on + strand, within Psest_0497 at 552.826 kb on + strand at 552.838 kb on + strand at 552.931 kb on + strand at 552.932 kb on - strand at 552.932 kb on - strand at 552.977 kb on + strand at 552.977 kb on + strand at 552.977 kb on + strand at 552.977 kb on + strand at 552.977 kb on + strand at 552.978 kb on - strand at 552.978 kb on - strand at 552.978 kb on - strand at 553.209 kb on - strand, within Psest_0498 at 553.396 kb on + strand, within Psest_0498 at 553.411 kb on + strand, within Psest_0498
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 551,069 + -1.0 551,069 + +0.8 551,070 - +6.6 551,070 - +3.7 551,075 + +1.2 551,075 + +0.8 551,075 + +0.7 551,075 + +0.8 551,076 - +2.5 551,293 - Psest_0496 0.25 +3.7 551,293 - Psest_0496 0.25 +3.1 551,293 - Psest_0496 0.25 +3.5 551,295 + Psest_0496 0.26 +3.2 551,295 + Psest_0496 0.26 +2.6 551,295 + Psest_0496 0.26 +3.0 551,320 - Psest_0496 0.29 +1.3 551,409 + Psest_0496 0.39 +1.9 551,409 + Psest_0496 0.39 +3.9 551,410 - Psest_0496 0.39 +2.1 551,410 - Psest_0496 0.39 +3.4 551,410 - Psest_0496 0.39 +3.6 551,457 + Psest_0496 0.45 +3.6 551,457 + Psest_0496 0.45 +3.5 551,458 - Psest_0496 0.45 +3.4 551,458 - Psest_0496 0.45 +0.4 551,458 - Psest_0496 0.45 +2.4 551,673 + Psest_0496 0.70 +1.6 551,725 - Psest_0496 0.76 +3.0 551,725 - Psest_0496 0.76 +0.3 551,725 - Psest_0496 0.76 +2.5 551,925 + -0.5 551,926 - -0.8 552,101 + Psest_0497 0.17 +3.4 552,101 + Psest_0497 0.17 +4.4 552,102 - Psest_0497 0.17 +3.5 552,112 - Psest_0497 0.18 +2.0 552,459 + Psest_0497 0.53 +3.8 552,459 + Psest_0497 0.53 +2.8 552,460 - Psest_0497 0.53 +3.0 552,460 - Psest_0497 0.53 +1.9 552,460 - Psest_0497 0.53 +4.2 552,460 - Psest_0497 0.53 +2.8 552,491 + Psest_0497 0.56 +2.8 552,492 - Psest_0497 0.57 +3.0 552,722 + Psest_0497 0.80 +4.1 552,826 + -0.1 552,838 + +0.4 552,931 + +0.5 552,932 - -0.2 552,932 - +0.7 552,977 + -2.2 552,977 + -1.5 552,977 + -1.5 552,977 + -1.5 552,977 + -0.7 552,978 - -0.7 552,978 - -3.0 552,978 - -0.3 553,209 - Psest_0498 0.27 +0.3 553,396 + Psest_0498 0.45 -0.7 553,411 + Psest_0498 0.47 -2.2
Or see this region's nucleotide sequence