Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21270 and BPHYT_RS21275 are separated by 105 nucleotides BPHYT_RS21275 and BPHYT_RS21280 are separated by 216 nucleotides BPHYT_RS21280 and BPHYT_RS21285 are separated by 323 nucleotides BPHYT_RS21285 and BPHYT_RS21290 are separated by 126 nucleotides
BPHYT_RS21270: BPHYT_RS21270 - dihydroxy-acid dehydratase, at 332,536 to 334,296
_RS21270
BPHYT_RS21275: BPHYT_RS21275 - short-chain dehydrogenase, at 334,402 to 335,007
_RS21275
BPHYT_RS21280: BPHYT_RS21280 - hypothetical protein, at 335,224 to 335,676
_RS21280
BPHYT_RS21285: BPHYT_RS21285 - hypothetical protein, at 336,000 to 336,233
_RS21285
BPHYT_RS21290: BPHYT_RS21290 - serine acetyltransferase, at 336,360 to 337,286
_RS21290
Position (kb) 335
336 Strain fitness (log2 ratio) -2
-1
0
1
2 at 334.273 kb on - strand at 334.514 kb on + strand, within BPHYT_RS21275 at 334.515 kb on - strand, within BPHYT_RS21275 at 334.515 kb on - strand, within BPHYT_RS21275 at 334.706 kb on + strand, within BPHYT_RS21275 at 334.707 kb on - strand, within BPHYT_RS21275 at 335.009 kb on + strand at 335.010 kb on - strand at 335.010 kb on - strand at 335.157 kb on - strand at 335.166 kb on - strand at 335.166 kb on - strand at 335.272 kb on - strand, within BPHYT_RS21280 at 335.331 kb on - strand, within BPHYT_RS21280 at 335.349 kb on - strand, within BPHYT_RS21280 at 335.536 kb on + strand, within BPHYT_RS21280 at 335.803 kb on + strand at 335.871 kb on - strand at 336.040 kb on + strand, within BPHYT_RS21285 at 336.084 kb on + strand, within BPHYT_RS21285 at 336.084 kb on + strand, within BPHYT_RS21285 at 336.085 kb on - strand, within BPHYT_RS21285 at 336.574 kb on - strand, within BPHYT_RS21290
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 334,273 - -1.4 334,514 + BPHYT_RS21275 0.18 +0.1 334,515 - BPHYT_RS21275 0.19 +0.6 334,515 - BPHYT_RS21275 0.19 -0.6 334,706 + BPHYT_RS21275 0.50 -0.6 334,707 - BPHYT_RS21275 0.50 -1.2 335,009 + +0.8 335,010 - +0.3 335,010 - +0.5 335,157 - +0.1 335,166 - +2.3 335,166 - -0.4 335,272 - BPHYT_RS21280 0.11 -0.6 335,331 - BPHYT_RS21280 0.24 +1.0 335,349 - BPHYT_RS21280 0.28 -0.2 335,536 + BPHYT_RS21280 0.69 +1.1 335,803 + +0.2 335,871 - -0.5 336,040 + BPHYT_RS21285 0.17 -1.9 336,084 + BPHYT_RS21285 0.36 -1.4 336,084 + BPHYT_RS21285 0.36 +0.0 336,085 - BPHYT_RS21285 0.36 -0.8 336,574 - BPHYT_RS21290 0.23 +0.4
Or see this region's nucleotide sequence