Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22005 and BPHYT_RS22010 are separated by 39 nucleotides BPHYT_RS22010 and BPHYT_RS22015 overlap by 8 nucleotides BPHYT_RS22015 and BPHYT_RS22020 are separated by 303 nucleotides
BPHYT_RS22005: BPHYT_RS22005 - ABC transporter permease, at 492,129 to 492,923
_RS22005
BPHYT_RS22010: BPHYT_RS22010 - ABC transporter permease, at 492,963 to 493,877
_RS22010
BPHYT_RS22015: BPHYT_RS22015 - spermidine/putrescine ABC transporter ATPase, at 493,870 to 494,961
_RS22015
BPHYT_RS22020: BPHYT_RS22020 - IclR family transcriptional regulator, at 495,265 to 496,143
_RS22020
Position (kb) 493
494
495 Strain fitness (log2 ratio) -1
0
1 at 493.006 kb on + strand at 493.470 kb on + strand, within BPHYT_RS22010 at 493.549 kb on + strand, within BPHYT_RS22010 at 493.550 kb on - strand, within BPHYT_RS22010 at 493.569 kb on + strand, within BPHYT_RS22010 at 493.630 kb on + strand, within BPHYT_RS22010 at 493.634 kb on - strand, within BPHYT_RS22010 at 494.307 kb on - strand, within BPHYT_RS22015 at 494.325 kb on + strand, within BPHYT_RS22015 at 494.590 kb on - strand, within BPHYT_RS22015 at 495.104 kb on + strand at 495.105 kb on - strand at 495.226 kb on - strand at 495.572 kb on + strand, within BPHYT_RS22020 at 495.841 kb on + strand, within BPHYT_RS22020
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 493,006 + +1.3 493,470 + BPHYT_RS22010 0.55 -0.6 493,549 + BPHYT_RS22010 0.64 -0.9 493,550 - BPHYT_RS22010 0.64 +0.9 493,569 + BPHYT_RS22010 0.66 -0.4 493,630 + BPHYT_RS22010 0.73 -0.3 493,634 - BPHYT_RS22010 0.73 -0.8 494,307 - BPHYT_RS22015 0.40 +0.5 494,325 + BPHYT_RS22015 0.42 -0.1 494,590 - BPHYT_RS22015 0.66 +1.7 495,104 + -0.5 495,105 - +0.7 495,226 - +1.0 495,572 + BPHYT_RS22020 0.35 -0.4 495,841 + BPHYT_RS22020 0.66 +0.9
Or see this region's nucleotide sequence