Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS36315 and BPHYT_RS36320 are separated by 2 nucleotides BPHYT_RS36320 and BPHYT_RS36325 are separated by 77 nucleotides BPHYT_RS36325 and BPHYT_RS36330 are separated by 201 nucleotides
BPHYT_RS36315: BPHYT_RS36315 - CagE TrbE VirB component of type IV transporter system, at 74,136 to 76,685
_RS36315
BPHYT_RS36320: BPHYT_RS36320 - type IV secretion system family protein, at 76,688 to 77,392
_RS36320
BPHYT_RS36325: BPHYT_RS36325 - TrbL/VirB6 plasmid conjugal transfer protein, at 77,470 to 78,468
_RS36325
BPHYT_RS36330: BPHYT_RS36330 - type IV secretory protein VirB8, at 78,670 to 79,569
_RS36330
Position (kb) 77
78
79 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 76.535 kb on - strand at 77.127 kb on - strand, within BPHYT_RS36320 at 77.127 kb on - strand, within BPHYT_RS36320 at 77.145 kb on - strand, within BPHYT_RS36320 at 77.713 kb on + strand, within BPHYT_RS36325 at 77.775 kb on + strand, within BPHYT_RS36325 at 77.805 kb on + strand, within BPHYT_RS36325 at 77.806 kb on - strand, within BPHYT_RS36325 at 78.022 kb on - strand, within BPHYT_RS36325 at 78.083 kb on - strand, within BPHYT_RS36325 at 78.085 kb on + strand, within BPHYT_RS36325 at 78.086 kb on - strand, within BPHYT_RS36325 at 78.137 kb on + strand, within BPHYT_RS36325 at 78.148 kb on + strand, within BPHYT_RS36325 at 78.148 kb on + strand, within BPHYT_RS36325 at 78.283 kb on + strand, within BPHYT_RS36325 at 78.283 kb on + strand, within BPHYT_RS36325 at 78.416 kb on - strand at 78.416 kb on - strand at 78.484 kb on - strand at 78.509 kb on - strand at 78.761 kb on + strand, within BPHYT_RS36330 at 78.762 kb on - strand, within BPHYT_RS36330 at 78.886 kb on + strand, within BPHYT_RS36330 at 78.887 kb on - strand, within BPHYT_RS36330 at 78.889 kb on + strand, within BPHYT_RS36330 at 78.889 kb on + strand, within BPHYT_RS36330 at 78.889 kb on + strand, within BPHYT_RS36330 at 78.890 kb on - strand, within BPHYT_RS36330 at 78.964 kb on + strand, within BPHYT_RS36330 at 79.012 kb on + strand, within BPHYT_RS36330 at 79.023 kb on - strand, within BPHYT_RS36330 at 79.066 kb on + strand, within BPHYT_RS36330 at 79.066 kb on + strand, within BPHYT_RS36330 at 79.066 kb on + strand, within BPHYT_RS36330 at 79.066 kb on + strand, within BPHYT_RS36330 at 79.067 kb on - strand, within BPHYT_RS36330 at 79.145 kb on - strand, within BPHYT_RS36330 at 79.207 kb on + strand, within BPHYT_RS36330 at 79.208 kb on - strand, within BPHYT_RS36330 at 79.442 kb on + strand, within BPHYT_RS36330 at 79.459 kb on + strand, within BPHYT_RS36330 at 79.459 kb on + strand, within BPHYT_RS36330
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 76,535 - +0.2 77,127 - BPHYT_RS36320 0.62 -0.8 77,127 - BPHYT_RS36320 0.62 -0.5 77,145 - BPHYT_RS36320 0.65 +0.0 77,713 + BPHYT_RS36325 0.24 -1.1 77,775 + BPHYT_RS36325 0.31 +0.6 77,805 + BPHYT_RS36325 0.34 -0.6 77,806 - BPHYT_RS36325 0.34 -0.1 78,022 - BPHYT_RS36325 0.55 -0.5 78,083 - BPHYT_RS36325 0.61 -1.7 78,085 + BPHYT_RS36325 0.62 -0.1 78,086 - BPHYT_RS36325 0.62 -0.0 78,137 + BPHYT_RS36325 0.67 +1.5 78,148 + BPHYT_RS36325 0.68 -1.3 78,148 + BPHYT_RS36325 0.68 -1.2 78,283 + BPHYT_RS36325 0.81 -2.5 78,283 + BPHYT_RS36325 0.81 +0.1 78,416 - +0.0 78,416 - +0.0 78,484 - -0.5 78,509 - -2.8 78,761 + BPHYT_RS36330 0.10 -1.0 78,762 - BPHYT_RS36330 0.10 +1.4 78,886 + BPHYT_RS36330 0.24 -0.0 78,887 - BPHYT_RS36330 0.24 +0.1 78,889 + BPHYT_RS36330 0.24 -0.3 78,889 + BPHYT_RS36330 0.24 -0.4 78,889 + BPHYT_RS36330 0.24 -0.7 78,890 - BPHYT_RS36330 0.24 -0.1 78,964 + BPHYT_RS36330 0.33 +1.6 79,012 + BPHYT_RS36330 0.38 -0.4 79,023 - BPHYT_RS36330 0.39 -0.3 79,066 + BPHYT_RS36330 0.44 +2.1 79,066 + BPHYT_RS36330 0.44 -0.3 79,066 + BPHYT_RS36330 0.44 +1.0 79,066 + BPHYT_RS36330 0.44 +0.3 79,067 - BPHYT_RS36330 0.44 +0.6 79,145 - BPHYT_RS36330 0.53 +0.4 79,207 + BPHYT_RS36330 0.60 +1.0 79,208 - BPHYT_RS36330 0.60 +0.1 79,442 + BPHYT_RS36330 0.86 -0.5 79,459 + BPHYT_RS36330 0.88 -0.2 79,459 + BPHYT_RS36330 0.88 -1.8
Or see this region's nucleotide sequence