Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35430 and BPHYT_RS35435 are separated by 186 nucleotides BPHYT_RS35435 and BPHYT_RS35440 are separated by 73 nucleotides BPHYT_RS35440 and BPHYT_RS35445 are separated by 29 nucleotides
BPHYT_RS35430: BPHYT_RS35430 - DSBA oxidoreductase, at 3,549,882 to 3,551,390
_RS35430
BPHYT_RS35435: BPHYT_RS35435 - glutathione S-transferase, at 3,551,577 to 3,552,254
_RS35435
BPHYT_RS35440: BPHYT_RS35440 - carboxymethylenebutenolidase, at 3,552,328 to 3,553,551
_RS35440
BPHYT_RS35445: BPHYT_RS35445 - 2-aminobenzoate-CoA ligase [EC: 6.2.1.32] (from data) , at 3,553,581 to 3,555,221
_RS35445
Position (kb)
3552
3553
3554 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3551.489 kb on - strand at 3551.525 kb on + strand at 3551.525 kb on + strand at 3551.552 kb on + strand at 3551.630 kb on - strand at 3551.797 kb on + strand, within BPHYT_RS35435 at 3551.956 kb on + strand, within BPHYT_RS35435 at 3551.968 kb on + strand, within BPHYT_RS35435 at 3551.969 kb on - strand, within BPHYT_RS35435 at 3552.211 kb on - strand at 3552.211 kb on - strand at 3552.353 kb on - strand at 3552.451 kb on + strand, within BPHYT_RS35440 at 3552.471 kb on - strand, within BPHYT_RS35440 at 3552.471 kb on - strand, within BPHYT_RS35440 at 3552.474 kb on + strand, within BPHYT_RS35440 at 3552.474 kb on + strand, within BPHYT_RS35440 at 3552.474 kb on + strand at 3552.475 kb on - strand, within BPHYT_RS35440 at 3552.475 kb on - strand, within BPHYT_RS35440 at 3552.475 kb on - strand, within BPHYT_RS35440 at 3552.486 kb on - strand, within BPHYT_RS35440 at 3552.536 kb on + strand, within BPHYT_RS35440 at 3552.585 kb on + strand, within BPHYT_RS35440 at 3552.586 kb on - strand, within BPHYT_RS35440 at 3552.592 kb on - strand, within BPHYT_RS35440 at 3552.661 kb on - strand, within BPHYT_RS35440 at 3552.755 kb on + strand, within BPHYT_RS35440 at 3552.756 kb on - strand, within BPHYT_RS35440 at 3552.848 kb on + strand, within BPHYT_RS35440 at 3552.848 kb on + strand, within BPHYT_RS35440 at 3552.894 kb on - strand, within BPHYT_RS35440 at 3552.921 kb on - strand, within BPHYT_RS35440 at 3553.385 kb on + strand, within BPHYT_RS35440 at 3553.582 kb on + strand at 3553.671 kb on - strand at 3554.472 kb on - strand, within BPHYT_RS35445 at 3554.505 kb on + strand, within BPHYT_RS35445 at 3554.505 kb on + strand, within BPHYT_RS35445 at 3554.506 kb on - strand, within BPHYT_RS35445 at 3554.506 kb on - strand, within BPHYT_RS35445 at 3554.506 kb on - strand, within BPHYT_RS35445
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 3,551,489 - +0.5 3,551,525 + -0.7 3,551,525 + -0.0 3,551,552 + -0.7 3,551,630 - +1.1 3,551,797 + BPHYT_RS35435 0.32 -0.3 3,551,956 + BPHYT_RS35435 0.56 +1.0 3,551,968 + BPHYT_RS35435 0.58 +1.9 3,551,969 - BPHYT_RS35435 0.58 -2.5 3,552,211 - +0.3 3,552,211 - +1.9 3,552,353 - -1.0 3,552,451 + BPHYT_RS35440 0.10 +2.0 3,552,471 - BPHYT_RS35440 0.12 -0.3 3,552,471 - BPHYT_RS35440 0.12 -0.3 3,552,474 + BPHYT_RS35440 0.12 -0.4 3,552,474 + BPHYT_RS35440 0.12 +0.4 3,552,474 + -0.5 3,552,475 - BPHYT_RS35440 0.12 +0.7 3,552,475 - BPHYT_RS35440 0.12 +1.0 3,552,475 - BPHYT_RS35440 0.12 -0.4 3,552,486 - BPHYT_RS35440 0.13 +0.5 3,552,536 + BPHYT_RS35440 0.17 -1.0 3,552,585 + BPHYT_RS35440 0.21 +0.9 3,552,586 - BPHYT_RS35440 0.21 -0.6 3,552,592 - BPHYT_RS35440 0.22 -2.3 3,552,661 - BPHYT_RS35440 0.27 +0.1 3,552,755 + BPHYT_RS35440 0.35 -1.3 3,552,756 - BPHYT_RS35440 0.35 +0.1 3,552,848 + BPHYT_RS35440 0.42 +0.2 3,552,848 + BPHYT_RS35440 0.42 -0.1 3,552,894 - BPHYT_RS35440 0.46 +0.2 3,552,921 - BPHYT_RS35440 0.48 +1.2 3,553,385 + BPHYT_RS35440 0.86 +0.5 3,553,582 + +0.2 3,553,671 - -2.5 3,554,472 - BPHYT_RS35445 0.54 -0.2 3,554,505 + BPHYT_RS35445 0.56 -0.5 3,554,505 + BPHYT_RS35445 0.56 +0.4 3,554,506 - BPHYT_RS35445 0.56 -0.3 3,554,506 - BPHYT_RS35445 0.56 +0.6 3,554,506 - BPHYT_RS35445 0.56 +0.2
Or see this region's nucleotide sequence