Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20215 and BPHYT_RS20220 are separated by 128 nucleotides BPHYT_RS20220 and BPHYT_RS20225 are separated by 87 nucleotides BPHYT_RS20225 and BPHYT_RS20230 overlap by 4 nucleotides BPHYT_RS20230 and BPHYT_RS20235 overlap by 4 nucleotides
BPHYT_RS20215: BPHYT_RS20215 - RNA polymerase sigma 70, at 80,806 to 81,477
_RS20215
BPHYT_RS20220: BPHYT_RS20220 - antibiotic synthesis protein MbtH, at 81,606 to 81,845
_RS20220
BPHYT_RS20225: BPHYT_RS20225 - taurine catabolism dioxygenase tauD/tfdA, at 81,933 to 82,967
_RS20225
BPHYT_RS20230: BPHYT_RS20230 - ABC transporter, at 82,964 to 83,830
_RS20230
BPHYT_RS20235: BPHYT_RS20235 - iron ABC transporter, at 83,827 to 85,935
_RS20235
Position (kb) 81
82
83 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 81.094 kb on + strand, within BPHYT_RS20215 at 81.094 kb on + strand, within BPHYT_RS20215 at 81.095 kb on - strand, within BPHYT_RS20215 at 81.507 kb on + strand at 81.664 kb on - strand, within BPHYT_RS20220 at 81.693 kb on + strand, within BPHYT_RS20220 at 81.933 kb on - strand at 82.029 kb on + strand at 82.097 kb on - strand, within BPHYT_RS20225 at 82.176 kb on + strand, within BPHYT_RS20225 at 82.221 kb on + strand, within BPHYT_RS20225 at 82.222 kb on - strand, within BPHYT_RS20225 at 82.222 kb on - strand, within BPHYT_RS20225 at 82.279 kb on - strand, within BPHYT_RS20225 at 82.302 kb on - strand, within BPHYT_RS20225 at 82.645 kb on + strand, within BPHYT_RS20225 at 82.729 kb on - strand, within BPHYT_RS20225 at 82.729 kb on - strand, within BPHYT_RS20225 at 82.914 kb on + strand at 82.915 kb on - strand at 83.809 kb on - strand at 83.884 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 81,094 + BPHYT_RS20215 0.43 -0.0 81,094 + BPHYT_RS20215 0.43 -0.2 81,095 - BPHYT_RS20215 0.43 -0.4 81,507 + +0.1 81,664 - BPHYT_RS20220 0.24 -0.6 81,693 + BPHYT_RS20220 0.36 -0.8 81,933 - +0.8 82,029 + -2.5 82,097 - BPHYT_RS20225 0.16 -1.2 82,176 + BPHYT_RS20225 0.23 -1.2 82,221 + BPHYT_RS20225 0.28 -4.0 82,222 - BPHYT_RS20225 0.28 -3.8 82,222 - BPHYT_RS20225 0.28 -1.6 82,279 - BPHYT_RS20225 0.33 -1.7 82,302 - BPHYT_RS20225 0.36 +0.3 82,645 + BPHYT_RS20225 0.69 -2.5 82,729 - BPHYT_RS20225 0.77 -1.9 82,729 - BPHYT_RS20225 0.77 -0.4 82,914 + -0.5 82,915 - -0.2 83,809 - -2.6 83,884 + -0.0
Or see this region's nucleotide sequence