Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS17305 and BPHYT_RS17310 are separated by 218 nucleotides BPHYT_RS17310 and BPHYT_RS17315 are separated by 189 nucleotides BPHYT_RS17315 and BPHYT_RS17320 are separated by 314 nucleotides BPHYT_RS17320 and BPHYT_RS17325 are separated by 68 nucleotides BPHYT_RS17325 and BPHYT_RS17330 are separated by 17 nucleotides
BPHYT_RS17305: BPHYT_RS17305 - amidohydrolase, at 3,922,516 to 3,923,712
_RS17305
BPHYT_RS17310: BPHYT_RS17310 - 5S ribosomal RNA, at 3,923,931 to 3,924,044
_RS17310
BPHYT_RS17315: BPHYT_RS17315 - 23S ribosomal RNA, at 3,924,234 to 3,927,136
_RS17315
BPHYT_RS17320: BPHYT_RS17320 - tRNA-Ala, at 3,927,451 to 3,927,526
_RS17320
BPHYT_RS17325: BPHYT_RS17325 - tRNA-Ile, at 3,927,595 to 3,927,671
_RS17325
BPHYT_RS17330: BPHYT_RS17330 - 16S ribosomal RNA, at 3,927,689 to 3,929,283
_RS17330
Position (kb)
3924
3925
3926
3927
3928 Strain fitness (log2 ratio)
-1
0
1 at 3923.304 kb on + strand, within BPHYT_RS17305 at 3923.305 kb on - strand, within BPHYT_RS17305 at 3923.363 kb on - strand, within BPHYT_RS17305 at 3923.630 kb on - strand at 3923.630 kb on - strand at 3923.710 kb on + strand at 3923.710 kb on + strand at 3923.710 kb on + strand at 3923.711 kb on - strand at 3923.711 kb on - strand at 3923.759 kb on - strand at 3923.804 kb on + strand at 3923.804 kb on + strand at 3923.805 kb on - strand at 3923.805 kb on - strand at 3923.805 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 3,923,304 + BPHYT_RS17305 0.66 -0.0 3,923,305 - BPHYT_RS17305 0.66 +0.8 3,923,363 - BPHYT_RS17305 0.71 -0.3 3,923,630 - +0.5 3,923,630 - -1.2 3,923,710 + -0.0 3,923,710 + -0.5 3,923,710 + -0.2 3,923,711 - +1.5 3,923,711 - +0.2 3,923,759 - -0.3 3,923,804 + -1.5 3,923,804 + -0.6 3,923,805 - +1.7 3,923,805 - -0.4 3,923,805 - -0.0
Or see this region's nucleotide sequence