Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13740 and BPHYT_RS13745 overlap by 4 nucleotides BPHYT_RS13745 and BPHYT_RS13750 are separated by 54 nucleotides BPHYT_RS13750 and BPHYT_RS13755 are separated by 50 nucleotides BPHYT_RS13755 and BPHYT_RS13760 are separated by 134 nucleotides
BPHYT_RS13740: BPHYT_RS13740 - NAD(P)H quinone oxidoreductase, at 3,131,410 to 3,132,012
_RS13740
BPHYT_RS13745: BPHYT_RS13745 - membrane protein, at 3,132,009 to 3,132,545
_RS13745
BPHYT_RS13750: BPHYT_RS13750 - D-2-hydroxyacid dehydrogenase, at 3,132,600 to 3,134,018
_RS13750
BPHYT_RS13755: BPHYT_RS13755 - metallophosphoesterase, at 3,134,069 to 3,134,875
_RS13755
BPHYT_RS13760: BPHYT_RS13760 - hypothetical protein, at 3,135,010 to 3,135,732
_RS13760
Position (kb)
3132
3133
3134
3135 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3132.044 kb on + strand at 3132.081 kb on - strand, within BPHYT_RS13745 at 3132.267 kb on + strand, within BPHYT_RS13745 at 3132.267 kb on + strand, within BPHYT_RS13745 at 3132.269 kb on + strand, within BPHYT_RS13745 at 3132.269 kb on + strand, within BPHYT_RS13745 at 3132.270 kb on - strand, within BPHYT_RS13745 at 3132.270 kb on - strand, within BPHYT_RS13745 at 3132.685 kb on - strand at 3132.685 kb on - strand at 3133.180 kb on + strand, within BPHYT_RS13750 at 3133.180 kb on + strand, within BPHYT_RS13750 at 3133.181 kb on - strand, within BPHYT_RS13750 at 3133.767 kb on + strand, within BPHYT_RS13750 at 3133.768 kb on - strand, within BPHYT_RS13750 at 3133.818 kb on + strand, within BPHYT_RS13750 at 3133.818 kb on + strand, within BPHYT_RS13750 at 3133.846 kb on - strand, within BPHYT_RS13750 at 3133.926 kb on + strand at 3133.927 kb on - strand at 3133.927 kb on - strand at 3133.928 kb on + strand at 3133.928 kb on + strand at 3133.929 kb on - strand at 3133.929 kb on - strand at 3133.929 kb on - strand at 3134.011 kb on + strand at 3134.012 kb on - strand at 3134.231 kb on + strand, within BPHYT_RS13755 at 3134.231 kb on + strand, within BPHYT_RS13755 at 3134.256 kb on - strand, within BPHYT_RS13755 at 3134.405 kb on + strand, within BPHYT_RS13755 at 3134.753 kb on - strand, within BPHYT_RS13755 at 3134.753 kb on - strand, within BPHYT_RS13755 at 3134.857 kb on + strand at 3134.871 kb on + strand at 3134.871 kb on + strand at 3134.873 kb on + strand at 3134.874 kb on - strand at 3134.874 kb on - strand at 3134.874 kb on - strand at 3134.932 kb on + strand at 3134.932 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 3,132,044 + +1.0 3,132,081 - BPHYT_RS13745 0.13 -1.1 3,132,267 + BPHYT_RS13745 0.48 -1.6 3,132,267 + BPHYT_RS13745 0.48 -1.6 3,132,269 + BPHYT_RS13745 0.48 -0.8 3,132,269 + BPHYT_RS13745 0.48 +0.5 3,132,270 - BPHYT_RS13745 0.49 -0.4 3,132,270 - BPHYT_RS13745 0.49 -0.3 3,132,685 - +0.4 3,132,685 - +0.6 3,133,180 + BPHYT_RS13750 0.41 +1.1 3,133,180 + BPHYT_RS13750 0.41 +0.5 3,133,181 - BPHYT_RS13750 0.41 -1.5 3,133,767 + BPHYT_RS13750 0.82 -1.3 3,133,768 - BPHYT_RS13750 0.82 +0.1 3,133,818 + BPHYT_RS13750 0.86 -0.8 3,133,818 + BPHYT_RS13750 0.86 +0.1 3,133,846 - BPHYT_RS13750 0.88 -1.0 3,133,926 + +2.4 3,133,927 - +0.6 3,133,927 - +2.6 3,133,928 + -1.3 3,133,928 + +0.4 3,133,929 - -0.2 3,133,929 - -0.3 3,133,929 - +0.9 3,134,011 + -0.9 3,134,012 - -0.1 3,134,231 + BPHYT_RS13755 0.20 +0.4 3,134,231 + BPHYT_RS13755 0.20 +0.2 3,134,256 - BPHYT_RS13755 0.23 -2.2 3,134,405 + BPHYT_RS13755 0.42 +0.3 3,134,753 - BPHYT_RS13755 0.85 +1.4 3,134,753 - BPHYT_RS13755 0.85 -0.4 3,134,857 + +0.5 3,134,871 + -1.4 3,134,871 + -0.4 3,134,873 + -0.4 3,134,874 - +0.2 3,134,874 - +0.5 3,134,874 - -0.7 3,134,932 + +0.4 3,134,932 + +0.2
Or see this region's nucleotide sequence