Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10005 and BPHYT_RS10010 are separated by 113 nucleotides BPHYT_RS10010 and BPHYT_RS10015 overlap by 4 nucleotides BPHYT_RS10015 and BPHYT_RS10020 are separated by 235 nucleotides BPHYT_RS10020 and BPHYT_RS10025 are separated by 70 nucleotides
BPHYT_RS10005: BPHYT_RS10005 - hypothetical protein, at 2,279,934 to 2,280,305
_RS10005
BPHYT_RS10010: BPHYT_RS10010 - cupin, at 2,280,419 to 2,280,850
_RS10010
BPHYT_RS10015: BPHYT_RS10015 - NAD(P)H dehydrogenase (quinone), at 2,280,847 to 2,281,530
_RS10015
BPHYT_RS10020: BPHYT_RS10020 - MFS transporter, at 2,281,766 to 2,282,203
_RS10020
BPHYT_RS10025: BPHYT_RS10025 - MarR family transcriptional regulator, at 2,282,274 to 2,283,788
_RS10025
Position (kb)
2280
2281
2282 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2280.111 kb on + strand, within BPHYT_RS10005 at 2280.111 kb on + strand, within BPHYT_RS10005 at 2280.363 kb on - strand at 2280.369 kb on - strand at 2281.059 kb on + strand, within BPHYT_RS10015 at 2281.060 kb on - strand, within BPHYT_RS10015 at 2281.079 kb on + strand, within BPHYT_RS10015 at 2281.179 kb on - strand, within BPHYT_RS10015 at 2281.245 kb on - strand, within BPHYT_RS10015 at 2281.245 kb on - strand, within BPHYT_RS10015 at 2281.245 kb on - strand, within BPHYT_RS10015 at 2281.580 kb on + strand at 2281.581 kb on - strand at 2281.679 kb on + strand at 2281.695 kb on - strand at 2282.202 kb on - strand at 2282.416 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 2,280,111 + BPHYT_RS10005 0.48 +0.1 2,280,111 + BPHYT_RS10005 0.48 -0.7 2,280,363 - -0.3 2,280,369 - -0.4 2,281,059 + BPHYT_RS10015 0.31 -2.2 2,281,060 - BPHYT_RS10015 0.31 -3.4 2,281,079 + BPHYT_RS10015 0.34 -2.7 2,281,179 - BPHYT_RS10015 0.49 -0.1 2,281,245 - BPHYT_RS10015 0.58 -1.5 2,281,245 - BPHYT_RS10015 0.58 -1.2 2,281,245 - BPHYT_RS10015 0.58 -2.2 2,281,580 + +2.8 2,281,581 - +0.1 2,281,679 + +0.2 2,281,695 - -1.9 2,282,202 - +0.0 2,282,416 - -0.0
Or see this region's nucleotide sequence