Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13575 and BPHYT_RS13580 are separated by 14 nucleotides BPHYT_RS13580 and BPHYT_RS13585 overlap by 4 nucleotides BPHYT_RS13585 and BPHYT_RS13590 are separated by 29 nucleotides BPHYT_RS13590 and BPHYT_RS13595 are separated by 154 nucleotides
BPHYT_RS13575: BPHYT_RS13575 - FAD-dependent oxidoreductase, at 3,097,325 to 3,098,617
_RS13575
BPHYT_RS13580: BPHYT_RS13580 - arginine ABC transporter ATP-binding protein, at 3,098,632 to 3,099,378
_RS13580
BPHYT_RS13585: BPHYT_RS13585 - amino acid ABC transporter permease, at 3,099,375 to 3,100,031
_RS13585
BPHYT_RS13590: BPHYT_RS13590 - ABC transporter substrate-binding protein, at 3,100,061 to 3,100,825
_RS13590
BPHYT_RS13595: BPHYT_RS13595 - IclR family transcriptional regulator, at 3,100,980 to 3,101,804
_RS13595
Position (kb)
3099
3100
3101 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3098.531 kb on - strand at 3098.992 kb on - strand, within BPHYT_RS13580 at 3098.992 kb on - strand, within BPHYT_RS13580 at 3098.992 kb on - strand, within BPHYT_RS13580 at 3099.241 kb on - strand, within BPHYT_RS13580 at 3099.424 kb on + strand at 3099.424 kb on + strand at 3099.425 kb on - strand at 3099.818 kb on + strand, within BPHYT_RS13585 at 3099.818 kb on + strand, within BPHYT_RS13585 at 3099.820 kb on + strand, within BPHYT_RS13585 at 3099.820 kb on + strand, within BPHYT_RS13585 at 3099.821 kb on - strand, within BPHYT_RS13585 at 3100.251 kb on + strand, within BPHYT_RS13590 at 3100.252 kb on - strand, within BPHYT_RS13590 at 3100.252 kb on - strand, within BPHYT_RS13590 at 3100.332 kb on + strand, within BPHYT_RS13590 at 3100.332 kb on + strand, within BPHYT_RS13590 at 3100.479 kb on + strand, within BPHYT_RS13590 at 3100.486 kb on - strand, within BPHYT_RS13590 at 3100.735 kb on - strand, within BPHYT_RS13590 at 3100.884 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 3,098,531 - -0.6 3,098,992 - BPHYT_RS13580 0.48 +0.3 3,098,992 - BPHYT_RS13580 0.48 -0.4 3,098,992 - BPHYT_RS13580 0.48 -0.8 3,099,241 - BPHYT_RS13580 0.82 -2.8 3,099,424 + +2.7 3,099,424 + +1.1 3,099,425 - -3.9 3,099,818 + BPHYT_RS13585 0.67 -1.0 3,099,818 + BPHYT_RS13585 0.67 -1.0 3,099,820 + BPHYT_RS13585 0.68 -3.5 3,099,820 + BPHYT_RS13585 0.68 -0.9 3,099,821 - BPHYT_RS13585 0.68 -0.7 3,100,251 + BPHYT_RS13590 0.25 -1.0 3,100,252 - BPHYT_RS13590 0.25 +0.0 3,100,252 - BPHYT_RS13590 0.25 -1.0 3,100,332 + BPHYT_RS13590 0.35 +0.8 3,100,332 + BPHYT_RS13590 0.35 -0.7 3,100,479 + BPHYT_RS13590 0.55 +0.6 3,100,486 - BPHYT_RS13590 0.56 -0.1 3,100,735 - BPHYT_RS13590 0.88 +1.2 3,100,884 + +0.9
Or see this region's nucleotide sequence