Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS27240 and BPHYT_RS27245 are separated by 330 nucleotides BPHYT_RS27245 and BPHYT_RS27250 are separated by 154 nucleotides BPHYT_RS27250 and BPHYT_RS27255 are separated by 102 nucleotides BPHYT_RS27255 and BPHYT_RS27260 are separated by 274 nucleotides
BPHYT_RS27240: BPHYT_RS27240 - N-ethylmaleimide reductase, at 1,687,049 to 1,688,152
_RS27240
BPHYT_RS27245: BPHYT_RS27245 - hypothetical protein, at 1,688,483 to 1,688,725
_RS27245
BPHYT_RS27250: BPHYT_RS27250 - NAD-dependent epimerase/dehydratase, at 1,688,880 to 1,689,518
_RS27250
BPHYT_RS27255: BPHYT_RS27255 - hypothetical protein, at 1,689,621 to 1,689,800
_RS27255
BPHYT_RS27260: BPHYT_RS27260 - membrane protein, at 1,690,075 to 1,690,605
_RS27260
Position (kb)
1688
1689
1690 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1687.953 kb on + strand, within BPHYT_RS27240 at 1688.115 kb on + strand at 1688.115 kb on + strand at 1688.315 kb on - strand at 1688.315 kb on - strand at 1688.356 kb on + strand at 1688.357 kb on - strand at 1688.424 kb on + strand at 1688.428 kb on + strand at 1689.145 kb on + strand, within BPHYT_RS27250 at 1689.320 kb on + strand, within BPHYT_RS27250 at 1689.838 kb on - strand at 1690.156 kb on + strand, within BPHYT_RS27260
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI remove 1,687,953 + BPHYT_RS27240 0.82 -0.8 1,688,115 + -0.3 1,688,115 + -1.8 1,688,315 - -1.8 1,688,315 - -0.1 1,688,356 + +0.1 1,688,357 - +0.1 1,688,424 + -1.1 1,688,428 + +1.2 1,689,145 + BPHYT_RS27250 0.41 -0.7 1,689,320 + BPHYT_RS27250 0.69 -1.6 1,689,838 - -0.0 1,690,156 + BPHYT_RS27260 0.15 +3.4
Or see this region's nucleotide sequence