Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18810 and BPHYT_RS18815 overlap by 8 nucleotides BPHYT_RS18815 and BPHYT_RS18820 are separated by 92 nucleotides BPHYT_RS18820 and BPHYT_RS18825 overlap by 1 nucleotides
BPHYT_RS18810: BPHYT_RS18810 - flagellar motor switch protein FliM, at 4,229,273 to 4,230,271
_RS18810
BPHYT_RS18815: BPHYT_RS18815 - flagellar motor switch protein FliN, at 4,230,264 to 4,230,728
_RS18815
BPHYT_RS18820: BPHYT_RS18820 - flagellar biosynthesis protein FliO, at 4,230,821 to 4,231,336
_RS18820
BPHYT_RS18825: BPHYT_RS18825 - flagellar biosynthesis protein FliP, at 4,231,336 to 4,232,169
_RS18825
Position (kb)
4230
4231 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4229.267 kb on + strand at 4229.268 kb on - strand at 4229.268 kb on - strand at 4229.268 kb on - strand at 4229.268 kb on - strand at 4229.385 kb on - strand, within BPHYT_RS18810 at 4229.537 kb on + strand, within BPHYT_RS18810 at 4229.617 kb on - strand, within BPHYT_RS18810 at 4229.765 kb on + strand, within BPHYT_RS18810 at 4229.765 kb on + strand, within BPHYT_RS18810 at 4229.765 kb on + strand, within BPHYT_RS18810 at 4229.765 kb on + strand, within BPHYT_RS18810 at 4229.765 kb on + strand, within BPHYT_RS18810 at 4229.766 kb on - strand, within BPHYT_RS18810 at 4229.766 kb on - strand, within BPHYT_RS18810 at 4229.917 kb on + strand, within BPHYT_RS18810 at 4229.917 kb on + strand, within BPHYT_RS18810 at 4229.918 kb on - strand, within BPHYT_RS18810 at 4229.918 kb on - strand, within BPHYT_RS18810 at 4229.918 kb on - strand, within BPHYT_RS18810 at 4229.918 kb on - strand, within BPHYT_RS18810 at 4229.918 kb on - strand, within BPHYT_RS18810 at 4230.207 kb on - strand at 4230.263 kb on + strand at 4230.264 kb on - strand at 4230.264 kb on - strand at 4230.692 kb on + strand at 4230.693 kb on - strand at 4230.824 kb on + strand at 4230.825 kb on - strand at 4231.115 kb on - strand, within BPHYT_RS18820 at 4231.284 kb on + strand, within BPHYT_RS18820 at 4231.284 kb on + strand, within BPHYT_RS18820 at 4231.547 kb on - strand, within BPHYT_RS18825
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI remove 4,229,267 + -2.1 4,229,268 - -0.6 4,229,268 - -0.4 4,229,268 - -2.7 4,229,268 - -1.7 4,229,385 - BPHYT_RS18810 0.11 -2.5 4,229,537 + BPHYT_RS18810 0.26 -1.4 4,229,617 - BPHYT_RS18810 0.34 -3.1 4,229,765 + BPHYT_RS18810 0.49 -2.7 4,229,765 + BPHYT_RS18810 0.49 -1.5 4,229,765 + BPHYT_RS18810 0.49 -0.7 4,229,765 + BPHYT_RS18810 0.49 -2.2 4,229,765 + BPHYT_RS18810 0.49 -3.4 4,229,766 - BPHYT_RS18810 0.49 -0.7 4,229,766 - BPHYT_RS18810 0.49 -4.7 4,229,917 + BPHYT_RS18810 0.64 -1.1 4,229,917 + BPHYT_RS18810 0.64 -0.3 4,229,918 - BPHYT_RS18810 0.65 -3.4 4,229,918 - BPHYT_RS18810 0.65 -3.8 4,229,918 - BPHYT_RS18810 0.65 -2.8 4,229,918 - BPHYT_RS18810 0.65 -2.1 4,229,918 - BPHYT_RS18810 0.65 -2.4 4,230,207 - -2.0 4,230,263 + -1.6 4,230,264 - -1.4 4,230,264 - -3.3 4,230,692 + -1.7 4,230,693 - -2.8 4,230,824 + +0.2 4,230,825 - -3.2 4,231,115 - BPHYT_RS18820 0.57 -3.8 4,231,284 + BPHYT_RS18820 0.90 -0.1 4,231,284 + BPHYT_RS18820 0.90 +1.1 4,231,547 - BPHYT_RS18825 0.25 -1.3
Or see this region's nucleotide sequence