Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15520 and BPHYT_RS15525 overlap by 4 nucleotides BPHYT_RS15525 and BPHYT_RS15530 are separated by 7 nucleotides BPHYT_RS15530 and BPHYT_RS15535 are separated by 123 nucleotides
BPHYT_RS15520: BPHYT_RS15520 - D-aminopeptidase, at 3,521,206 to 3,522,306
_RS15520
BPHYT_RS15525: BPHYT_RS15525 - glutathione ABC transporter permease GsiD, at 3,522,303 to 3,523,208
_RS15525
BPHYT_RS15530: BPHYT_RS15530 - glutathione ABC transporter permease, at 3,523,216 to 3,524,136
_RS15530
BPHYT_RS15535: BPHYT_RS15535 - glutathione ABC transporter substrate-binding protein GsiB, at 3,524,260 to 3,525,822
_RS15535
Position (kb)
3523
3524
3525 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3522.545 kb on - strand, within BPHYT_RS15525 at 3522.633 kb on - strand, within BPHYT_RS15525 at 3522.634 kb on + strand, within BPHYT_RS15525 at 3522.634 kb on + strand, within BPHYT_RS15525 at 3522.634 kb on + strand, within BPHYT_RS15525 at 3522.634 kb on + strand, within BPHYT_RS15525 at 3522.634 kb on + strand, within BPHYT_RS15525 at 3522.635 kb on - strand, within BPHYT_RS15525 at 3522.832 kb on + strand, within BPHYT_RS15525 at 3522.835 kb on + strand, within BPHYT_RS15525 at 3522.836 kb on - strand, within BPHYT_RS15525 at 3523.250 kb on + strand at 3523.250 kb on + strand at 3523.619 kb on + strand, within BPHYT_RS15530 at 3523.619 kb on + strand, within BPHYT_RS15530 at 3523.619 kb on + strand, within BPHYT_RS15530 at 3523.619 kb on + strand, within BPHYT_RS15530 at 3523.620 kb on - strand, within BPHYT_RS15530 at 3524.052 kb on + strand at 3524.052 kb on + strand at 3524.053 kb on - strand at 3524.053 kb on - strand at 3524.053 kb on - strand at 3524.187 kb on + strand at 3524.309 kb on + strand at 3524.310 kb on - strand at 3524.483 kb on + strand, within BPHYT_RS15535 at 3524.582 kb on + strand, within BPHYT_RS15535 at 3524.586 kb on - strand, within BPHYT_RS15535 at 3524.586 kb on - strand, within BPHYT_RS15535 at 3524.733 kb on - strand, within BPHYT_RS15535 at 3524.738 kb on + strand, within BPHYT_RS15535 at 3524.931 kb on - strand, within BPHYT_RS15535 at 3524.992 kb on - strand, within BPHYT_RS15535 at 3524.993 kb on + strand, within BPHYT_RS15535 at 3524.994 kb on - strand, within BPHYT_RS15535
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI remove 3,522,545 - BPHYT_RS15525 0.27 -0.3 3,522,633 - BPHYT_RS15525 0.36 +0.3 3,522,634 + BPHYT_RS15525 0.37 -0.5 3,522,634 + BPHYT_RS15525 0.37 +0.5 3,522,634 + BPHYT_RS15525 0.37 +0.9 3,522,634 + BPHYT_RS15525 0.37 +2.6 3,522,634 + BPHYT_RS15525 0.37 -0.2 3,522,635 - BPHYT_RS15525 0.37 +0.5 3,522,832 + BPHYT_RS15525 0.58 -0.4 3,522,835 + BPHYT_RS15525 0.59 +1.0 3,522,836 - BPHYT_RS15525 0.59 +0.1 3,523,250 + +0.1 3,523,250 + -0.1 3,523,619 + BPHYT_RS15530 0.44 +0.9 3,523,619 + BPHYT_RS15530 0.44 -1.0 3,523,619 + BPHYT_RS15530 0.44 +0.1 3,523,619 + BPHYT_RS15530 0.44 +4.1 3,523,620 - BPHYT_RS15530 0.44 +0.5 3,524,052 + +0.3 3,524,052 + +0.3 3,524,053 - -0.3 3,524,053 - -1.4 3,524,053 - -0.1 3,524,187 + +0.1 3,524,309 + +0.2 3,524,310 - +0.4 3,524,483 + BPHYT_RS15535 0.14 +0.4 3,524,582 + BPHYT_RS15535 0.21 -1.8 3,524,586 - BPHYT_RS15535 0.21 -2.9 3,524,586 - BPHYT_RS15535 0.21 +0.2 3,524,733 - BPHYT_RS15535 0.30 -0.3 3,524,738 + BPHYT_RS15535 0.31 -3.0 3,524,931 - BPHYT_RS15535 0.43 +0.3 3,524,992 - BPHYT_RS15535 0.47 -0.7 3,524,993 + BPHYT_RS15535 0.47 +0.7 3,524,994 - BPHYT_RS15535 0.47 -1.7
Or see this region's nucleotide sequence