Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14175 and BPHYT_RS14180 are separated by 145 nucleotides BPHYT_RS14180 and BPHYT_RS14185 are separated by 20 nucleotides BPHYT_RS14185 and BPHYT_RS14190 are separated by 151 nucleotides
BPHYT_RS14175: BPHYT_RS14175 - RNA-binding protein, at 3,237,864 to 3,238,391
_RS14175
BPHYT_RS14180: BPHYT_RS14180 - membrane protein, at 3,238,537 to 3,239,046
_RS14180
BPHYT_RS14185: BPHYT_RS14185 - transcription elongation factor GreA, at 3,239,067 to 3,239,543
_RS14185
BPHYT_RS14190: BPHYT_RS14190 - carbamoyl phosphate synthase large subunit, at 3,239,695 to 3,242,949
_RS14190
Position (kb)
3239
3240 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3238.103 kb on + strand, within BPHYT_RS14175 at 3238.104 kb on - strand, within BPHYT_RS14175 at 3238.390 kb on - strand at 3238.502 kb on + strand at 3238.503 kb on - strand at 3238.649 kb on + strand, within BPHYT_RS14180 at 3238.695 kb on - strand, within BPHYT_RS14180 at 3238.753 kb on - strand, within BPHYT_RS14180 at 3238.983 kb on - strand, within BPHYT_RS14180 at 3239.074 kb on + strand at 3239.074 kb on + strand at 3239.075 kb on - strand at 3239.095 kb on + strand at 3239.231 kb on - strand, within BPHYT_RS14185 at 3239.544 kb on - strand at 3239.554 kb on - strand at 3239.554 kb on - strand at 3239.590 kb on - strand at 3239.657 kb on - strand at 3239.669 kb on + strand at 3239.670 kb on - strand at 3239.670 kb on - strand at 3239.696 kb on + strand at 3239.722 kb on + strand at 3239.796 kb on - strand at 3239.798 kb on + strand at 3239.799 kb on - strand at 3239.799 kb on - strand at 3239.939 kb on - strand at 3240.260 kb on + strand, within BPHYT_RS14190 at 3240.261 kb on - strand, within BPHYT_RS14190 at 3240.261 kb on - strand, within BPHYT_RS14190 at 3240.365 kb on + strand, within BPHYT_RS14190 at 3240.366 kb on - strand, within BPHYT_RS14190 at 3240.366 kb on - strand, within BPHYT_RS14190 at 3240.366 kb on - strand, within BPHYT_RS14190 at 3240.407 kb on + strand, within BPHYT_RS14190
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI remove 3,238,103 + BPHYT_RS14175 0.45 -1.0 3,238,104 - BPHYT_RS14175 0.45 -0.7 3,238,390 - -0.4 3,238,502 + +0.7 3,238,503 - +0.9 3,238,649 + BPHYT_RS14180 0.22 -2.1 3,238,695 - BPHYT_RS14180 0.31 +1.3 3,238,753 - BPHYT_RS14180 0.42 +2.2 3,238,983 - BPHYT_RS14180 0.87 +2.4 3,239,074 + +0.4 3,239,074 + -0.6 3,239,075 - -0.8 3,239,095 + +0.9 3,239,231 - BPHYT_RS14185 0.34 -1.7 3,239,544 - -0.8 3,239,554 - +0.3 3,239,554 - -0.2 3,239,590 - -0.6 3,239,657 - -0.5 3,239,669 + +0.2 3,239,670 - +1.0 3,239,670 - +0.4 3,239,696 + -1.9 3,239,722 + -0.2 3,239,796 - -1.2 3,239,798 + -3.1 3,239,799 - -0.8 3,239,799 - -2.5 3,239,939 - +2.8 3,240,260 + BPHYT_RS14190 0.17 -2.1 3,240,261 - BPHYT_RS14190 0.17 -2.9 3,240,261 - BPHYT_RS14190 0.17 -2.6 3,240,365 + BPHYT_RS14190 0.21 -3.9 3,240,366 - BPHYT_RS14190 0.21 -2.2 3,240,366 - BPHYT_RS14190 0.21 -3.5 3,240,366 - BPHYT_RS14190 0.21 -1.5 3,240,407 + BPHYT_RS14190 0.22 -3.0
Or see this region's nucleotide sequence