Experiment: R2A control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31050 and BPHYT_RS31055 are separated by 30 nucleotides BPHYT_RS31055 and BPHYT_RS31060 are separated by 47 nucleotides BPHYT_RS31060 and BPHYT_RS31065 are separated by 7 nucleotides
BPHYT_RS31050: BPHYT_RS31050 - epimerase, at 2,521,458 to 2,522,597
_RS31050
BPHYT_RS31055: BPHYT_RS31055 - glycosyl transferase, at 2,522,628 to 2,523,494
_RS31055
BPHYT_RS31060: BPHYT_RS31060 - capsular polysaccharide biosynthesis protein, at 2,523,542 to 2,524,822
_RS31060
BPHYT_RS31065: BPHYT_RS31065 - polysaccharide biosynthesis protein, at 2,524,830 to 2,526,194
_RS31065
Position (kb)
2523
2524
2525 Strain fitness (log2 ratio)
-2
-1
0
1 at 2522.595 kb on + strand at 2523.259 kb on - strand, within BPHYT_RS31055 at 2523.573 kb on - strand at 2524.097 kb on - strand, within BPHYT_RS31060 at 2524.230 kb on - strand, within BPHYT_RS31060 at 2525.391 kb on + strand, within BPHYT_RS31065 at 2525.394 kb on - strand, within BPHYT_RS31065 at 2525.395 kb on - strand, within BPHYT_RS31065 at 2525.684 kb on - strand, within BPHYT_RS31065
Per-strain Table
Position Strand Gene LocusTag Fraction R2A control remove 2,522,595 + -0.5 2,523,259 - BPHYT_RS31055 0.73 -0.0 2,523,573 - -1.2 2,524,097 - BPHYT_RS31060 0.43 -2.1 2,524,230 - BPHYT_RS31060 0.54 -0.5 2,525,391 + BPHYT_RS31065 0.41 +0.9 2,525,394 - BPHYT_RS31065 0.41 -0.8 2,525,395 - BPHYT_RS31065 0.41 -1.5 2,525,684 - BPHYT_RS31065 0.63 -0.2
Or see this region's nucleotide sequence