Experiment: R2A control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24965 and BPHYT_RS24970 are separated by 64 nucleotides BPHYT_RS24970 and BPHYT_RS24975 are separated by 193 nucleotides BPHYT_RS24975 and BPHYT_RS24980 overlap by 4 nucleotides BPHYT_RS24980 and BPHYT_RS24985 overlap by 4 nucleotides
BPHYT_RS24965: BPHYT_RS24965 - hypothetical protein, at 1,173,155 to 1,173,475
_RS24965
BPHYT_RS24970: BPHYT_RS24970 - hypothetical protein, at 1,173,540 to 1,173,641
_RS24970
BPHYT_RS24975: BPHYT_RS24975 - RNA polymerase sigma24 factor, at 1,173,835 to 1,174,395
_RS24975
BPHYT_RS24980: BPHYT_RS24980 - hypothetical protein, at 1,174,392 to 1,174,820
_RS24980
BPHYT_RS24985: BPHYT_RS24985 - membrane protein, at 1,174,817 to 1,175,335
_RS24985
Position (kb) 1173
1174
1175 Strain fitness (log2 ratio) -1
0
1 at 1172.950 kb on + strand at 1173.139 kb on - strand at 1173.363 kb on - strand, within BPHYT_RS24965 at 1173.634 kb on + strand at 1173.637 kb on - strand at 1173.649 kb on + strand at 1173.649 kb on + strand at 1174.255 kb on + strand, within BPHYT_RS24975 at 1174.256 kb on - strand, within BPHYT_RS24975 at 1174.259 kb on - strand, within BPHYT_RS24975 at 1175.379 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A control remove 1,172,950 + -0.4 1,173,139 - +0.9 1,173,363 - BPHYT_RS24965 0.65 -0.5 1,173,634 + -0.1 1,173,637 - -0.5 1,173,649 + +0.8 1,173,649 + -0.1 1,174,255 + BPHYT_RS24975 0.75 +0.7 1,174,256 - BPHYT_RS24975 0.75 +1.5 1,174,259 - BPHYT_RS24975 0.76 +0.6 1,175,379 + +0.6
Or see this region's nucleotide sequence