Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25065 and BPHYT_RS25070 overlap by 8 nucleotides BPHYT_RS25070 and BPHYT_RS25075 are separated by 7 nucleotides BPHYT_RS25075 and BPHYT_RS25080 are separated by 88 nucleotides
BPHYT_RS25065: BPHYT_RS25065 - glycine/betaine ABC transporter ATPase, at 1,194,200 to 1,195,378
_RS25065
BPHYT_RS25070: BPHYT_RS25070 - choline ABC transporter permease, at 1,195,371 to 1,196,273
_RS25070
BPHYT_RS25075: BPHYT_RS25075 - AraC family transcriptional regulator, at 1,196,281 to 1,197,279
_RS25075
BPHYT_RS25080: BPHYT_RS25080 - glycine/betaine ABC transporter substrate-binding protein, at 1,197,368 to 1,198,303
_RS25080
Position (kb) 1196
1197
1198 Strain fitness (log2 ratio) -2
-1
0
1 at 1195.310 kb on + strand at 1195.311 kb on - strand at 1195.608 kb on + strand, within BPHYT_RS25070 at 1196.228 kb on + strand at 1196.423 kb on - strand, within BPHYT_RS25075 at 1197.110 kb on - strand, within BPHYT_RS25075 at 1197.669 kb on + strand, within BPHYT_RS25080 at 1197.998 kb on + strand, within BPHYT_RS25080
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 1,195,310 + -0.7 1,195,311 - -1.0 1,195,608 + BPHYT_RS25070 0.26 +0.2 1,196,228 + -0.1 1,196,423 - BPHYT_RS25075 0.14 +0.3 1,197,110 - BPHYT_RS25075 0.83 -1.4 1,197,669 + BPHYT_RS25080 0.32 -2.2 1,197,998 + BPHYT_RS25080 0.67 -1.4
Or see this region's nucleotide sequence