Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS15675

Experiment: R2A with Vogels_fungal_media 0.8X

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS15665 and BPHYT_RS15670 overlap by 4 nucleotidesBPHYT_RS15670 and BPHYT_RS15675 are separated by 117 nucleotidesBPHYT_RS15675 and BPHYT_RS15680 are separated by 172 nucleotides BPHYT_RS15665: BPHYT_RS15665 - SAM-dependent methyltransferase, at 3,552,469 to 3,553,728 _RS15665 BPHYT_RS15670: BPHYT_RS15670 - phosphoribosylglycinamide formyltransferase, at 3,553,725 to 3,554,378 _RS15670 BPHYT_RS15675: BPHYT_RS15675 - riboflavin kinase, at 3,554,496 to 3,555,491 _RS15675 BPHYT_RS15680: BPHYT_RS15680 - isoleucine--tRNA ligase, at 3,555,664 to 3,558,501 _RS15680 Position (kb) 3554 3555 3556Strain fitness (log2 ratio) -3 -2 -1 0 1at 3553.782 kb on - strandat 3553.782 kb on - strandat 3553.847 kb on - strand, within BPHYT_RS15670at 3553.851 kb on - strand, within BPHYT_RS15670at 3553.851 kb on - strand, within BPHYT_RS15670at 3554.077 kb on + strand, within BPHYT_RS15670at 3554.148 kb on - strand, within BPHYT_RS15670at 3554.390 kb on - strandat 3554.390 kb on - strandat 3554.445 kb on - strandat 3554.445 kb on - strandat 3554.445 kb on - strandat 3555.485 kb on + strandat 3555.485 kb on + strandat 3555.485 kb on + strandat 3555.638 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X
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3,553,782 - -1.9
3,553,782 - +0.0
3,553,847 - BPHYT_RS15670 0.19 -1.6
3,553,851 - BPHYT_RS15670 0.19 -2.2
3,553,851 - BPHYT_RS15670 0.19 -3.4
3,554,077 + BPHYT_RS15670 0.54 -2.0
3,554,148 - BPHYT_RS15670 0.65 -1.1
3,554,390 - +0.0
3,554,390 - -1.5
3,554,445 - -0.3
3,554,445 - +0.8
3,554,445 - +0.1
3,555,485 + +0.4
3,555,485 + +0.3
3,555,485 + -0.2
3,555,638 + -1.1

Or see this region's nucleotide sequence