Experiment: MBOA 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2167 and Psyr_2168 are separated by 3 nucleotides Psyr_2168 and Psyr_2169 overlap by 20 nucleotides Psyr_2169 and Psyr_RNA40 are separated by 88 nucleotides Psyr_RNA40 and Psyr_2170 are separated by 158 nucleotides
Psyr_2167: Psyr_2167 - phenylalanyl-tRNA synthetase beta subunit, at 2,519,998 to 2,522,376
_2167
Psyr_2168: Psyr_2168 - Integration host factor, alpha subunit, at 2,522,380 to 2,522,682
_2168
Psyr_2169: Psyr_2169 - transcriptional regulator, MerR family, at 2,522,663 to 2,523,019
_2169
Psyr_RNA40: Psyr_RNA40 - tRNA-Pro, at 2,523,108 to 2,523,184
_RNA40
Psyr_2170: Psyr_2170 - L-glutamate-binding protein / L-aspartate-binding protein, at 2,523,343 to 2,524,257
_2170
Position (kb)
2522
2523
2524 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2522.374 kb on + strand at 2522.419 kb on + strand, within Psyr_2168 at 2522.439 kb on + strand, within Psyr_2168 at 2522.503 kb on - strand, within Psyr_2168 at 2522.662 kb on + strand at 2522.662 kb on + strand at 2522.662 kb on + strand at 2522.662 kb on + strand at 2522.663 kb on - strand at 2522.663 kb on - strand at 2522.680 kb on + strand at 2522.680 kb on + strand at 2522.714 kb on + strand, within Psyr_2169 at 2522.714 kb on + strand, within Psyr_2169 at 2522.715 kb on - strand, within Psyr_2169 at 2522.715 kb on - strand, within Psyr_2169 at 2522.771 kb on + strand, within Psyr_2169 at 2522.877 kb on + strand, within Psyr_2169 at 2522.877 kb on + strand, within Psyr_2169 at 2522.878 kb on - strand, within Psyr_2169 at 2522.879 kb on + strand at 2522.880 kb on - strand, within Psyr_2169 at 2522.880 kb on - strand, within Psyr_2169 at 2522.954 kb on + strand, within Psyr_2169 at 2522.954 kb on + strand, within Psyr_2169 at 2522.955 kb on - strand, within Psyr_2169 at 2522.955 kb on - strand, within Psyr_2169 at 2522.955 kb on - strand, within Psyr_2169 at 2522.997 kb on + strand at 2522.997 kb on + strand at 2522.998 kb on - strand at 2522.998 kb on - strand at 2522.998 kb on - strand at 2523.063 kb on - strand at 2523.096 kb on + strand at 2523.096 kb on + strand at 2523.097 kb on - strand at 2523.186 kb on - strand at 2523.187 kb on + strand at 2523.188 kb on - strand at 2523.188 kb on - strand at 2523.288 kb on - strand at 2523.454 kb on - strand, within Psyr_2170 at 2523.463 kb on - strand, within Psyr_2170 at 2523.467 kb on + strand, within Psyr_2170 at 2523.468 kb on - strand, within Psyr_2170 at 2523.504 kb on + strand, within Psyr_2170 at 2523.505 kb on - strand, within Psyr_2170 at 2523.505 kb on - strand, within Psyr_2170 at 2523.505 kb on - strand, within Psyr_2170 at 2523.563 kb on + strand, within Psyr_2170 at 2523.563 kb on + strand, within Psyr_2170 at 2523.564 kb on - strand, within Psyr_2170 at 2523.657 kb on + strand, within Psyr_2170 at 2523.658 kb on - strand, within Psyr_2170 at 2523.658 kb on - strand, within Psyr_2170 at 2523.692 kb on + strand, within Psyr_2170 at 2523.693 kb on - strand, within Psyr_2170 at 2523.721 kb on + strand, within Psyr_2170 at 2523.721 kb on + strand, within Psyr_2170 at 2523.721 kb on + strand, within Psyr_2170 at 2523.790 kb on - strand, within Psyr_2170 at 2523.797 kb on - strand, within Psyr_2170 at 2523.831 kb on - strand, within Psyr_2170 at 2523.831 kb on - strand, within Psyr_2170 at 2523.995 kb on + strand, within Psyr_2170 at 2523.995 kb on + strand, within Psyr_2170 at 2523.996 kb on - strand, within Psyr_2170 at 2523.996 kb on - strand, within Psyr_2170 at 2524.000 kb on + strand, within Psyr_2170 at 2524.000 kb on + strand, within Psyr_2170 at 2524.000 kb on + strand, within Psyr_2170 at 2524.001 kb on - strand, within Psyr_2170
Per-strain Table
Position Strand Gene LocusTag Fraction MBOA 0.5 mM remove 2,522,374 + +0.2 2,522,419 + Psyr_2168 0.13 -1.6 2,522,439 + Psyr_2168 0.19 +0.8 2,522,503 - Psyr_2168 0.41 -0.0 2,522,662 + -2.1 2,522,662 + -0.3 2,522,662 + -0.3 2,522,662 + -1.5 2,522,663 - -1.2 2,522,663 - -1.2 2,522,680 + -0.5 2,522,680 + -0.6 2,522,714 + Psyr_2169 0.14 +0.3 2,522,714 + Psyr_2169 0.14 +0.1 2,522,715 - Psyr_2169 0.15 -1.4 2,522,715 - Psyr_2169 0.15 +0.1 2,522,771 + Psyr_2169 0.30 +1.0 2,522,877 + Psyr_2169 0.60 -0.4 2,522,877 + Psyr_2169 0.60 -1.5 2,522,878 - Psyr_2169 0.60 -0.0 2,522,879 + +0.6 2,522,880 - Psyr_2169 0.61 -0.3 2,522,880 - Psyr_2169 0.61 -0.5 2,522,954 + Psyr_2169 0.82 +0.4 2,522,954 + Psyr_2169 0.82 -0.9 2,522,955 - Psyr_2169 0.82 +0.5 2,522,955 - Psyr_2169 0.82 -0.1 2,522,955 - Psyr_2169 0.82 +0.9 2,522,997 + +0.1 2,522,997 + -0.1 2,522,998 - +0.9 2,522,998 - -1.3 2,522,998 - +0.2 2,523,063 - +0.9 2,523,096 + -0.8 2,523,096 + -0.4 2,523,097 - -0.4 2,523,186 - -3.1 2,523,187 + +1.7 2,523,188 - +0.8 2,523,188 - -1.5 2,523,288 - +0.0 2,523,454 - Psyr_2170 0.12 +0.3 2,523,463 - Psyr_2170 0.13 -1.1 2,523,467 + Psyr_2170 0.14 +0.8 2,523,468 - Psyr_2170 0.14 -0.7 2,523,504 + Psyr_2170 0.18 -0.3 2,523,505 - Psyr_2170 0.18 +1.4 2,523,505 - Psyr_2170 0.18 +0.0 2,523,505 - Psyr_2170 0.18 +0.1 2,523,563 + Psyr_2170 0.24 +0.2 2,523,563 + Psyr_2170 0.24 +0.5 2,523,564 - Psyr_2170 0.24 -1.2 2,523,657 + Psyr_2170 0.34 +1.4 2,523,658 - Psyr_2170 0.34 -1.2 2,523,658 - Psyr_2170 0.34 -0.0 2,523,692 + Psyr_2170 0.38 +2.2 2,523,693 - Psyr_2170 0.38 -0.0 2,523,721 + Psyr_2170 0.41 +0.2 2,523,721 + Psyr_2170 0.41 +0.4 2,523,721 + Psyr_2170 0.41 +1.1 2,523,790 - Psyr_2170 0.49 -0.1 2,523,797 - Psyr_2170 0.50 -0.3 2,523,831 - Psyr_2170 0.53 +0.4 2,523,831 - Psyr_2170 0.53 +0.7 2,523,995 + Psyr_2170 0.71 -0.8 2,523,995 + Psyr_2170 0.71 -0.2 2,523,996 - Psyr_2170 0.71 -0.3 2,523,996 - Psyr_2170 0.71 -0.5 2,524,000 + Psyr_2170 0.72 +0.3 2,524,000 + Psyr_2170 0.72 -0.6 2,524,000 + Psyr_2170 0.72 +0.6 2,524,001 - Psyr_2170 0.72 -0.6
Or see this region's nucleotide sequence